期刊
BIOTECHNOLOGY AND BIOENGINEERING
卷 99, 期 4, 页码 1028-1033出版社
JOHN WILEY & SONS INC
DOI: 10.1002/bit.21649
关键词
microreactors; screening; enzyme catalysis; lyases; crude lysates
We have demonstrated that multiple crude enzyme lysates containing a hydroxynitrile lyase can be used for the enantioselective synthesis of cyanohydrins from aldehydes in microchannels. Using a microreactor setup, two important parameters were efficiently screened consuming only minute amounts of reagents. More importantly, results from the continuous flow reaction were fully consistent with results obtained from larger batchwise processes in which a stable emulsion was formed.
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