Article
Chemistry, Medicinal
Abdulaziz A. Aldubayyan, Erika Castrignano, Simon Elliott, Vincenzo Abbate
Summary: This study presents a validated LC-MS/MS method for detecting and quantifying synthetic cathinones and their metabolites in human urine. Analysis of suspicious urine samples from Saudi Arabia revealed the presence of synthetic cathinones, specifically mephedrone and N-ethylpentylone, as well as their dihydro-metabolites, along with other classical stimulant drugs. This is the first report of new psychoactive substance use in Saudi Arabia involving designer stimulant drugs. The findings highlight the need for broader urine analysis to detect and report otherwise undetected or new analytes.
Article
Multidisciplinary Sciences
F. Ivy Carroll, Anita H. Lewin, S. Wayne Mascarella, Herbert H. Seltzman, P. Anantha Reddy
Summary: The review discusses the exponential increase in clandestine manufacture of new psychoactive substances designed to circumvent regulations, which has led to a rise in fatalities. It focuses on three compound classes and highlights the medicinal chemistry approaches used by clandestine laboratories to develop new substances, as well as the chemical methods for treating illicit drug abuse.
ANNALS OF THE NEW YORK ACADEMY OF SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Nuria Nadal-Gratacos, Ana Sofia Alberto-Silva, Miriam Rodriguez-Soler, Edurne Urquizu, Maria Espinosa-Velasco, Kathrin Jaentsch, Marion Holy, Xavier Batllori, Xavier Berzosa, David Pubill, Jordi Camarasa, Harald H. Sitte, Elena Escubedo, Raul Lopez-Arnau
Summary: The study investigated the structure-activity relationships of six novel synthetic cathinones, revealing potent dopamine uptake inhibition and varying effects on serotonin transporters. These synthetic cathinones exhibited different psychostimulant effects and rewarding properties in mice, providing valuable information for understanding their mechanisms of action in addiction.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Legal
Shu-Yu Fan, Chi-Zong Zang, Po-Han Shih, Ya-Chun Ko, Ya-Hui Hsu, Mei-Chih Lin, Su-Hsiang Tseng, Der-Yuan Wang
Summary: The abuse of new psychoactive substances poses risks to public health and safety, with phenethylamines being the main reported substances. A study introduced an LC-MS/MS method for detecting 74 conventional and artificial phenethylamines in urine samples, which was validated through the analysis of authentic urine samples, showing 20 samples tested positive for seven phenethylamines.
FORENSIC SCIENCE INTERNATIONAL
(2021)
Article
Chemistry, Analytical
Ivan Langa, Maria Elizabeth Tiritan, Diana Silva, Claudia Ribeiro
Summary: The study developed and validated an indirect method for the enantiomeric quantification of psychoactive substances in surface waters using gas chromatography coupled to mass spectrometry. The method allowed for the evaluation and monitoring of target substances in Portuguese surface waters and effluents, revealing the presence of illicit synthetic cathinones and other substances in the Douro River estuary for the first time. The potential of the method for monitoring the consumption of target substances was demonstrated.
Article
Biochemistry & Molecular Biology
Elisabet Teixido, Clara Riera-Colomer, Demetrio Raldua, David Pubill, Elena Escubedo, Marta Barenys, Raul Lopez-Arnau
Summary: This study evaluates the cardiovascular toxicity of synthetic cathinones and traditional psychostimulants in zebrafish embryos. The results show that all drugs can induce bradycardia, arrhythmia, and atrial-ventricular block, with MDPV being the most potent in inducing these effects.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Pharmacology & Pharmacy
N. La Maida, G. Mannocchi, S. Pichini, G. Basile, A. Di Giorgi, F. P. Busardo, E. Marchei
Summary: A targeted screening and quantification method for synthetic cathinones (SCs) in hair samples was developed and validated. The method showed good selectivity, specificity, and easy and low-cost sample preparation. The most prevalent SCs in hair samples from SCs consumers were 3,4-Methylenedioxy-alpha-Pyrrolidinohexanophenone, alpha-Pyrrolidinohexiophenone, 3-Methylmethcathinone, and 4-Methylethcathinone.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2022)
Article
Biochemical Research Methods
Yan Shi, Mengxi Liu, Xiangjun Li, Niusheng Xu, Shuai Yuan, Zhiguo Yu, Ping Xiang, Hejian Wu
Summary: Synthetic cannabinoids are a group of new psychoactive substances that can cause various symptoms and even death when abused. An efficient liquid chromatography-high resolution mass spectrometry method is crucial for their identification and analysis.
JOURNAL OF CHROMATOGRAPHY A
(2022)
Article
Substance Abuse
Pei-Ying Lee, Chun-Chi Hsu, Chia-Hsiang Chan
Summary: The case of a patient who developed acute myocarditis and subsequent psychotic symptoms after ingesting mixed synthetic cathinones highlights the need for research into the association between synthetic cathinones and psychosis and myocarditis. The lack of clinical data and potential dire outcomes necessitate further exploration.
JOURNAL OF ADDICTION MEDICINE
(2023)
Review
Neurosciences
Davide Radaelli, Alessandro Manfredi, Martina Zanon, Paolo Fattorini, Matteo Scopetti, Margherita Neri, Paolo Frisoni, Stefano D'Errico
Summary: New psychoactive substances (NPS) are a group of psychotropic substances designed to mimic traditional substances' effects, not regulated by UN Conventions. These substances pose challenges for public health and forensic toxicologists, causing a wide range of side effects involving various systems and mechanisms of action. Studies primarily focus on neurotoxic effects, with limited research on cardiotoxicity. Synthetic cannabinoids and cathinones play a role in cardiac events through different mechanisms, primarily mediated by CB1 receptor activation, leading to concerns about adrenergic over-stimulation and oxidative stress. This systematic review aims to summarize research on how synthetic cannabinoids and cathinones damage myocardial tissue.
CURRENT NEUROPHARMACOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Gloria Daziani, Alfredo Fabrizio Lo Faro, Vincenzo Montana, Gaia Goteri, Mauro Pesaresi, Giulia Bambagiotti, Eva Montanari, Raffaele Giorgetti, Angelo Montana
Summary: According to the EU Early Warning System (EWS), synthetic cathinones (SCs) are the second largest class of new psychoactive substances (NPS), with 162 types being monitored. They have a structure similar to cathinone, found in Catha Edulis, and exhibit stimulant effects similar to amphetamines.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Medical Laboratory Technology
Ju-Yu Chen, Guan-Yuan Chen, Hooi-Nee Ong, Mei-Ling Lai, Yi-Ju Ho, Ching-Hua Kuo, Te- Weng
Summary: This study found that blood collection tubes have a significant impact on the recoveries of synthetic cathinones, especially serum separating tubes and gel-containing tubes. However, in real-world scenarios, nonideal collection methods are inevitably employed, so it is important to rigorously interpret forensic toxicological results, especially for susceptible species.
CLINICA CHIMICA ACTA
(2023)
Article
Biochemistry & Molecular Biology
Chiara Lavarello, Sebastiano Barco, Martina Bartolucci, Isabella Panfoli, Emanuele Magi, Gino Tripodi, Andrea Petretto, Giuliana Cangemi
Summary: LC-HRMS is the preferred method for untargeted metabolomic analysis, with established protocols to ensure accurate metabolite identification. The study successfully built an AMRT database using a commercial metabolite library, achieving level 1 identification of metabolites in pediatric plasma and urine samples, providing a foundation for future metabolomic studies in pediatric settings.
Article
Environmental Sciences
Heon-Jun Lee, Jeong-Eun Oh
Summary: This study aimed to develop a target and suspect screening method using LC-HRMS to identify new psychoactive substances (NPS) in wastewater samples. A database of 95 traditional and NPS was built, and an analytical method was developed. Analysis of wastewater samples from 29 treatment plants identified 14 substances, including 3 NPS and 11 traditional psychoactive substances. This study highlights the need for continuous monitoring of NPS in South Korea.
SCIENCE OF THE TOTAL ENVIRONMENT
(2023)
Article
Pharmacology & Pharmacy
Hyeri Park, Mingliang Lin, Jian Zhou, Lisa M. Eubanks, Bin Zhou, Kim D. Janda
Summary: Opioid use disorders and overdose have become a major public health concern. U-47700, a new psychoactive substances opioid, is identified as a new threat in the drug supply due to its potency and abuse potential. The development of a U-47700 vaccine has shown promising results in rodents, generating antibodies that effectively mitigate drug-induced effects and prevent the drug from entering the brain.
FRONTIERS IN PHARMACOLOGY
(2023)
Review
Biochemical Research Methods
Yi He, Marta Concheiro-Guisan
BIOMEDICAL CHROMATOGRAPHY
(2019)
Article
Chemistry, Analytical
Victoria Mei, Marta Concheiro, Justine Pardi, Gail Cooper
JOURNAL OF ANALYTICAL TOXICOLOGY
(2019)
Article
Biochemical Research Methods
Angela Lopez-Rabunal, Elena Lendoiro, Marta Concheiro, Manuel Lopez-Rivadulla, Angelines Cruz, Ana de-Castro-Rios
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2019)
Article
Medicine, Legal
Rachel Chesser, Justine Pardi, Marta Concheiro, Gail Cooper
FORENSIC SCIENCE INTERNATIONAL
(2019)
Article
Chemistry, Analytical
A. Lopez-Rabunal, E. Lendoiro, M. Concheiro, M. Lopez-Rivadulla, A. Cruz, A. de-Castro-Rios
JOURNAL OF ANALYTICAL TOXICOLOGY
(2020)
Article
Chemistry, Analytical
Angela Lopez-Rabunal, Elena Lendoiro, Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Marta Concheiro-Guisan, Patricia Penas-Silva, Manuel Macias-Cortina, Manuel Lopez-Rivadulla, Angelines Cruz, Ana de-Castro-Rios
JOURNAL OF ANALYTICAL TOXICOLOGY
(2020)
Article
Chemistry, Analytical
Timothy Lau, Marta Concheiro, Gail Cooper
JOURNAL OF ANALYTICAL TOXICOLOGY
(2020)
Article
Chemistry, Analytical
Natalia A. Platosz, Tina M. Binz, Markus R. Baumgartner, Elena Lendoiro, Ana de Castro, Marta Concheiro
Summary: The use and misuse of synthetic and prescription opioids in the USA has become an epidemic, leading to a high number of deaths and cases of opioid use disorder. Hair is now being increasingly used in forensic toxicology to investigate drug use patterns due to its long window of detection. A method was developed to detect and quantify 27 classic, prescription, and synthetic opioids in hair using LC-MS-MS.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2021)
Article
Medicine, Legal
Steven Towler, Marta Concheiro, Sue Pearring, Luke N. Rodda
Summary: Performing point-of-care urine drug screen testing at autopsy by a forensic pathologist may offer early indication of presence of analytes; Evaluation of Alere iCup DX 14 for screening postmortem urine drug abuse; Sensitivity and specificity of iCup for various drug classes in comparison with confirmatory tests.
JOURNAL OF FORENSIC SCIENCES
(2021)
Article
Neurosciences
Britta Hahn, Alexander N. Harvey, Marta Concheiro-Guisan, Marilyn A. Huestis, Thomas J. Ross, Elliot A. Stein
Summary: This study found that nAChR antagonist can weaken task-induced deactivation of the DMN, indicating that the constant activation tone of nAChR typically helps regulate DMN activity in healthy individuals. This implies that low nAChR tone may play a causal role in DMN dysregulation seen in conditions such as mild cognitive impairment or Alzheimer's disease.
PSYCHOPHARMACOLOGY
(2021)
Article
Biochemical Research Methods
Marta Concheiro, Frank Martin Gutierrez, Alejandro Ocampo, Elena Lendoiro, Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Patricia Penas-Silva, Manuel Macias-Cortina, Angelines Cruz-Landeira, Manuel Lopez-Rivadulla, Ana de-Castro-Rios
Summary: Analyzing prenatal marijuana exposure in paired hair, meconium, umbilical cord, and placental samples revealed complementarity between meconium and hair, with higher agreement with meconium for umbilical cord and placental results. THCCOOH-glucuronide in umbilical cord and placenta can be a biomarker of cannabis exposure, offering a potential alternative to meconium.
DRUG TESTING AND ANALYSIS
(2021)
Article
Toxicology
Angela Lopez-Rabunal, Elena Lendoiro, Marta Concheiro-Guisan, Eva Gonzalez-Colmenero, Patricia Penas-Silva, Ana Concheiro-Guisan, Manuel Macia-Cortinas, Manuel Lopez-Rivadulla, Ana De-Castro-Rios, Angelines Cruz
Summary: This study compared meconium, maternal hair, and maternal medical records to detect exposure to antidepressants and benzodiazepines during pregnancy. Maternal hair was more sensitive in detecting antidepressant use, while meconium analysis was better at providing detection of neonatal benzodiazepines exposure at delivery. No significant influence of prenatal exposure to these drugs on neonatal outcomes was observed.
FORENSIC TOXICOLOGY
(2021)
Article
Chemistry, Analytical
Laura DeFreitas, Ana Miguel Fonseca Pego, Robert Kronstrand, Elena Lendoiro, Ana De Castro-Rios, Marta Concheiro
Summary: Identification and analysis of designer benzodiazepines have become a challenge in forensic toxicology. A fast and sensitive method using LC-MS/MS was developed and validated to determine 17 designer benzodiazepines in hair samples.
JOURNAL OF ANALYTICAL TOXICOLOGY
(2022)
Article
Substance Abuse
Eva Gonzalez-Colmenero, Ana Concheiro-Guisan, Marta Lorenzo-Martinez, Marta Concheiro, Elena Lendoiro, Ana de-Castro-Rios, Debora Canizo-Vazquez, Cristina Martinez-Reglero, Jose Ramon Fernandez-Lorenzo
JOURNAL OF ADDICTIVE DISEASES
(2020)
Article
Medicine, Legal
Nicole Centazzo, Bonnie-Marie Frederick, Alethea Jacox, Shu-Yuan Cheng, Marta Concheiro-Guisan
FORENSIC SCIENCES RESEARCH
(2019)
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)