期刊
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
卷 57, 期 -, 页码 167-175出版社
WILEY
DOI: 10.1042/BA20100212
关键词
biomass; bioreactor; carbon dioxide; fermentation; ventilation flask; unsteady model
In the present study, a new online monitoring method for the determination of the CO2 sensitivity of micro-organisms, based on the values of the respiration factors [OTR (oxygen transfer rate) and CTR (carbon dioxide transfer rate)], obtained by using the RAMOS (respiratory activity monitoring system) device considering a variety of aeration rates in the measuring flask, is investigated. Based on the data of the OTR, obtained by RAMOS under a variety of specific aeration rates, the proposed new method was developed as an online monitoring method for CO2 sensitivity of micro-organisms in shaken bioreactors. A maximum accumulated CO2 concentration of 12% was derived in applied methods, provided that the cultivation system is carried out under optimal conditions. Additionally, to predict these conditions, an unsteady-state gas transfer model in shaken bioreactors would be very advantageous. The data of OTR obtained using the RAMOS device were analysed and recalculated by a programme considering the calibration factor (C-f). The major advantage of the new method is the possibility to determine the metabolic activity, regardless of manual sampling.
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