Article
Cardiac & Cardiovascular Systems
Dien Ye, Xiaofei Yang, Liwei Ren, Hong S. Lu, Yuan Sun, Hui Lin, Lunbo Tan, Na Wang, Genevieve Nguyen, Michael Bader, Adam E. Mullick, A. H. Jan Danser, Alan Daugherty, Yizhou Jiang, Yidan Sun, Furong Li, Xifeng Lu
Summary: Inhibiting hepatic (P)RR can effectively reduce plasma cholesterol and triglyceride concentrations, but does not necessarily decrease atherosclerosis development. Transcriptomic analysis in the aortas revealed increased expression of genes related to immune responses and inflammation with (P)RR inhibition, while downregulating (P)RR in macrophages enhanced inflammatory responses and accelerated atherosclerosis.
FRONTIERS IN CARDIOVASCULAR MEDICINE
(2021)
Article
Pharmacology & Pharmacy
Urszula Tyrankiewicz, Mariola Olkowicz, Piotr Berkowicz, Magdalena Jablonska, Ryszard T. Smolenski, Jerzy A. Zoladz, Stefan Chlopicki
Summary: The study found that combined spontaneous physical activity and ACE-I treatment had additive beneficial effects on ACE/ACE-2 balance in a mouse model of heart failure, but did not result in improvement in cardiac function.
FRONTIERS IN PHARMACOLOGY
(2021)
Article
Medicine, Research & Experimental
Bradley L. Ackermann, Ryan D. Morrison, Salisha Hill, Matthew D. Westfall, Brent D. Butts, Michael D. Soper, Jeff A. Fill, Andrew E. Schade, Daniel C. Liebler, Aaron M. Gruver
Summary: MS analysis allows accurate protein quantification from stained paraffin-embedded tissue sections, providing valuable data for the assessment of clinical tissue specimens.
LABORATORY INVESTIGATION
(2023)
Article
Biochemistry & Molecular Biology
Bibi S. van Thiel, Janette van der Linden, Yanto Ridwan, Ingrid M. Garrelds, Marcel Vermeij, Marian C. Clahsen-van Groningen, Fatimunnisa Qadri, Natalia Alenina, Michael Bader, Anton J. M. Roks, A. H. Jan Danser, Jeroen Essers, Ingrid van der Pluijm
Summary: Changes in the renin-angiotensin system may contribute to aging and age-related diseases, while the use of a renin-activatable probe can help detect increased renin activity in progeroid mice, aiding in the study of kidney disease progression and treatment.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Endocrinology & Metabolism
Amanda da Silva Chaves, Nathalia Santos Magalhaes, Daniella Bianchi Reis Insuela, Patricia Machado Rodrigues E Silva, Marco Aurelio Martins, Vinicius Frias Carvalho
Summary: This study investigates the role of the renin-angiotensin system (RAS) in the overproduction of corticosterone in diabetic mice. The results suggest that inhibition of angiotensin synthesis and AT(1) receptor activity can reduce corticosterone levels in diabetic mice. Furthermore, the protective effect of Olmesartan on corticosterone overproduction in diabetic mice depends on both AT(1) receptor blockade and AT(2) receptor activation.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Cardiac & Cardiovascular Systems
Ruurt A. Jukema, Ruben W. de Winter, Pepijn A. van Diemen, Roel S. Driessen, A. H. Jan Danser, Ingrid M. Garrelds, Pieter G. Raijmakers, Peter M. van de Ven, Paul Knaapen, Ibrahim Danad, Guus A. de Waard
Summary: This study investigated the relationship between renin angiotensin aldosterone system (RAAS) activation and endothelin-1 levels with coronary artery calcium (CAC) score, total plaque volume (TPV), high-risk plaque, hyperemic myocardial blood flow (MBF), and coronary microvascular dysfunction (CMD). The study found that renin levels were positively associated with CAC score and TPV, while endothelin-1 levels were not significantly related to these parameters.
Article
Cell Biology
Tian-Yi Zhu, Shang-Tong Li, Dan-Dan Liu, Xiajun Zhang, Lianqi Zhou, Rong Zhou, Bing Yang
Summary: This study quantifies protein changes during aging in different individuals using single-worm mass spectrometry, and reveals the heterogeneity of aging by reconstructing the aging trajectory and proteomic landscape of C. elegans.
Article
Urology & Nephrology
Benedikt Marahrens, Arndt Schulze, Jan Wysocki, Meei-Hua Lin, Minghao Ye, Yashpal S. Kanwar, Michael Bader, Juan Carlos Q. Velez, Jeffrey H. Miner, Daniel Batlle
Summary: The study found that aminopeptidase A deficiency results in increased blood pressure and altered glomerular structure in mice, but without significant proteinuria. Additionally, the activity, protein, and mRNA levels of certain enzymes in the renin-angiotensin system in the kidney may be regulated compensatory in knockout mice.
KIDNEY INTERNATIONAL
(2021)
Article
Hematology
Jeff Z. Chen, Hisashi Sawada, Dien Ye, Yuriko Katsumata, Masayoshi Kukida, Satoko Ohno-Urabe, Jessica J. Moorleghen, Michael K. Franklin, Deborah A. Howatt, Mary B. Sheppard, Adam E. Mullick, Hong S. Lu, Alan Daugherty
Summary: Deletion of AT1aR and inhibition of Ang II production had similar effects in attenuating pathologies in the proximal thoracic aorta of male Fbn1(C1041G/+) mice. Inhibition of the renin-angiotensin system attenuated dysregulation of genes within the aorta related to pathology of Fbn1(C1041G/+) mice.
ARTERIOSCLEROSIS THROMBOSIS AND VASCULAR BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Danielle Carvalho Oliveira Coutinho, Julliane Joviano-Santos, Artur Santos -Miranda, Paulo Antonio Martins-Junior, Analina Da Silva, Robson Augusto Souza Santos, Anderson Jose Ferreira
Summary: The activation of the novel axis of the renin-angiotensin system (ACE2/Ang-(1-7)/Mas receptor) has been found to have protective effects against cardiovascular diseases. This study investigated the implications of Mas receptor knockout on cytokine profiles, energy metabolism, and electrical properties of isolated cardiomyocytes in mice.
BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS
(2022)
Article
Biochemical Research Methods
Filipe Alex da Silva, Lucas Rodrigues-Ribeiro, Marcella Nunes Melo-Braga, Danielle Gomes Passos-Silva, Walkyria Oliveira Sampaio, Vladimir Gorshkov, Frank Kjeldsen, Thiago Verano-Braga, Robson Augusto Souza Santos
Summary: This study investigates the signaling pathways of alamandine in both tumor and non-tumor cells, revealing its antiproliferative effect by activating the transcription factor FoxO1, negatively regulating the PI3K/AKT/mTOR pathway, and inducing metabolic shift in tumor cells.
Article
Biochemical Research Methods
Kenji Egashira, Yoko Ino, Yusuke Nakai, Takashi Ohira, Tomoko Akiyama, Kayano Moriyama, Yu Yamamoto, Mitsuo Kimura, Akihide Ryo, Tomoyuki Saito, Yutaka Inaba, Hisashi Hirano, Ken Kumagai, Yayoi Kimura
Summary: A high-sensitivity analysis system for mouse bone proteins was established using data-independent acquisition mass spectrometry. 40 proteins showing differential abundance in a microgravity environment were detected, as well as 22 proteins maintaining similar abundance under mastication stimuli. The mechanical stimulus-responsive protein SPARCL1 was suggested to be involved in bone remodeling. These findings contribute to understanding the mechanism of bone metabolism induced by mechanical stimuli.
JOURNAL OF PROTEOMICS
(2023)
Article
Biochemical Research Methods
Paolo Cifani, Alex Kentsis
Summary: To better understand cellular signaling and achieve quantitative measurements, researchers have developed the Quantitative Cell Proteomics Atlas (QCPA). Using targeted mass spectrometry assays, QCPA allows for analysis of most known signaling pathways and their post-translational modifications, providing valuable insights into cellular processes.
JOURNAL OF PROTEOME RESEARCH
(2022)
Article
Plant Sciences
Tulake Wuniqiemu, Jingjing Qin, Fangzhou Teng, Mohammadtursun Nabijan, Jie Cui, La Yi, Weifeng Tang, Xueyi Zhu, Muhammadjan Abduwaki, Mammat Nurahmat, Ying Wei, Jing cheng Dong
Summary: The study confirmed the anti-inflammatory and anti-fibrotic role of LKZP decoction in airway, with ECM-receptor interaction being potentially involved. RMB 10 and COL6A6 were identified as potential targets of LKZP decoction in OVA-induced asthmatic mice.
JOURNAL OF ETHNOPHARMACOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Laszlo Prokai, Fatima Rahlouni, Khadiza Zaman, Vien Nguyen, Katalin Prokai-Tatrai
Summary: The study identified 165 uterine proteins significantly regulated by estrogen exposure using untargeted quantitative proteomics, with a focus on molecular targets related to cellular functions. Key molecular nodes, such as nuclear estrogen receptors, were confirmed to play important roles in hormone-associated events. The combination of untargeted and targeted proteomic strategies demonstrated the potential for identifying candidate molecular markers for evaluating endocrine-disrupting chemicals.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Miroslaw Zagaja, Jaroslaw Bryda, Aleksandra Szewczyk, Joanna Szala-Rycaj, Jarogniew J. Luszczki, Maria Walczak, Kamil Kus, Marta Andres-Mach
Summary: The study showed that XANT enhances the antiepileptic activity of levetiracetam and valproate without affecting motor coordination or long-term memory. It had no impact on levetiracetam concentrations in the brain, but increased valproate concentrations, indicating different pharmacokinetic interactions between the two drugs.
FUNDAMENTAL & CLINICAL PHARMACOLOGY
(2022)
Article
Chemistry, Analytical
Karolina Matyjaszczyk-Gwarda, Agnieszka Kij, Mariola Olkowicz, Benedikt Fels, Kristina Kusche-Vihrog, Maria Walczak, Stefan Chlopicki
Summary: A novel LC-SRM/MS-based method was developed for accurate profiling of glycosaminoglycans (GAGs), providing important information on the status of the endothelial glycocalyx in cell culture and in vivo conditions.
Article
Biology
Patrycja Jablonska, Paulina Mierzejewska, Marta Tomczyk, Patrycja Koszalka, Marika Franczak, Ada Kawecka, Barbara Kutryb-Zajac, Alicja Braczko, Ryszard T. Smolenski, Ewa M. Slominska
Summary: This study demonstrates significant differences in extracellular NAD(+) and NMN metabolism in vascular endothelial cells from different species and locations, which may impact strategies for modulating the NAD(+) system and complicate result comparisons. Abnormalities in extracellular NAD(+) and NMN metabolism may affect related signaling cascades and pathologies.
Review
Biochemistry & Molecular Biology
Agata Jedrzejewska, Alicja Braczko, Ada Kawecka, Marcin Hellmann, Piotr Siondalski, Ewa Slominska, Barbara Kutryb-Zajac, Magdi H. Yacoub, Ryszard T. Smolenski
Summary: LVAD therapy is an effective rescue in acute and chronic cardiac failure, providing a platform for myocardial recovery. The combination of LVAD support and pharmacotherapy can enhance cardiomyocyte recovery and improve patient outcomes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Alicja Braczko, Barbara Kutryb-Zajac, Agata Jedrzejewska, Oliwia Krol, Paulina Mierzejewska, Magdalena Zabielska-Kaczorowska, Ewa M. Slominska, Ryszard T. Smolenski
Summary: This study reveals that cardiac mitochondrial dysfunction develops during prolonged mild hyperlipidemia at the time point corresponding to the formation of early vascular alterations.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Magdalena A. Zabielska-Kaczorowska, Ryszard T. Smolenski
Summary: In this experiment, novel solutions for organ preservation were compared and Transmedium Transplant Fluid (TTF) demonstrated better protection of cardiac function and nucleotide metabolite release during cold storage and reperfusion.
NUCLEOSIDES NUCLEOTIDES & NUCLEIC ACIDS
(2022)
Article
Cell Biology
Marta Tomczyk, Alicja Braczko, Paulina Mierzejewska, Magdalena Podlacha, Oliwia Krol, Patrycja Jablonska, Agata Jedrzejewska, Karolina Pierzynowska, Grzegorz Wegrzyn, Ewa M. Slominska, Ryszard T. Smolenski
Summary: In this study, the effects of rosiglitazone on grip strength and heart function in an experimental HD model were tested. It was found that rosiglitazone treatment improved grip strength and cardiac mechanical function in R6/1 mice by enhancing cellular energetics.
Article
Biochemistry & Molecular Biology
Elzbieta Sucajtys-Szulc, Alicja Debska-Slizien, Boleslaw Rutkowski, Ryszard Milczarek, Marek Szolkiewicz, Julian Swierczynski, Ryszard Tomasz Smolenski
Summary: Chronic kidney disease (CKD) is associated with elevated plasma fibrinogen concentration, with HNF1a gene upregulation leading to increased fibrinogen gene expression. The mRNA levels of liver fibrinogen and HNF1a are closely related to the progression of kidney disease. Decreasing HNF1a gene expression can reduce plasma fibrinogen concentration.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Federica Borea, Marika A. Franczak, Maria Garcia, Matteo Perrino, Nadia Cordua, Ryszard T. Smolenski, Godefridus J. Peters, Rafal Dziadziuszko, Armando Santoro, Paolo A. Zucali, Elisa Giovannetti
Summary: Malignant Pleural Mesothelioma (MPM) is a rare neoplasm that is usually diagnosed at an advanced stage and requires systemic treatment due to its ineligibility for radical surgery. Chemotherapy has been the standard treatment for 20 years until immune checkpoint inhibitors were introduced. However, the prognosis remains poor, and targeted therapies for MPM have mostly failed in clinical trials. This review aims to explore the potential reasons for treatment failures and determine the need for further research in this area.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Marika A. Franczak, Oliwia Krol, Gabriela Harasim, Agata Jedrzejewska, Nadia Zaffaroni, Carlotta Granchi, Filippo Minutolo, Amir Avan, Elisa Giovannetti, Ryszard T. Smolenski, Godefridus J. Peters
Summary: Malignant mesothelioma (MM) is a highly aggressive and resistant tumor. In this study, the cytotoxicity of new inhibitors of glucose transporter type 1 (GLUT-1) and lactate dehydrogenase-A (LDH-A) in relation to ATP/NAD+ metabolism, glycolysis and mitochondrial respiration was investigated. The inhibitors showed cytotoxicity in MM cells, associated with a decrease in ATP and NAD+, and were most effective in cells with the highest metabolic modulation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Multidisciplinary Sciences
Justyna Mojsa-Kaja, Klaudia Szklarczyk-Smolana, Ewa Niedzielska-Andres, Anna Kurpinska, Joanna Suraj-Prazmowska, Maria Walczak
Summary: The COVID-19 pandemic has resulted in prolonged social isolation and its negative impact on sleep and mental health. This study examines the role of insomnia as a mediator between social isolation and mental health outcomes, showing that insomnia mediates the relationship between social isolation and anxiety and depression.
Review
Biochemistry & Molecular Biology
Patrycja Maszka, Magdalena Kwasniak-Butowska, Dominik Cysewski, Jaroslaw Slawek, Ryszard T. Smolenski, Marta Tomczyk
Summary: The prevalence of neurodegenerative diseases (NDs) is increasing due to demographic changes. This review explores the alterations in brain metabolism in Alzheimer's disease (AD), Parkinson's disease (PD), and Huntington's disease (HD). Based on the findings, potential metabolic indicators or predictors of the diseases are proposed. Challenges and future directions of metabolomics studies in NDs are discussed. The integration of metabolomics with other omic techniques may be necessary to achieve clinically applicable results.
Article
Chemistry, Medicinal
Magdalena A. Zabielska-Kaczorowska, Alicja Braczko, Iwona Pelikant-Malecka, Ewa M. Slominska, Ryszard T. Smolenski
Summary: Myocardial ischemia leads to a decrease in adenosine production, which affects its protective functions. This study tested the relationship between cardiac adenine nucleotide pool (TAN) and adenosine production. Results showed that after 1 min ischemia at 85 min, adenosine production decreased to less than 15% of that at 40 min, accompanied by a decrease in ATP and TAN. However, when adenosine was infused after 10 min ischemia, adenosine production was partially restored. These findings suggest that only a fraction of the cardiac adenine nucleotide pool is available for adenosine production.
Article
Cell Biology
Ewelina Czuba-Pakula, Iwona Pelikant-Malecka, Grazyna Lietzau, Slawomir Wojcik, Ryszard T. Smolenski, Przemyslaw Kowianski
Summary: Hypercholesterolemia affects the glycolytic efficiency and energy metabolism of cerebral blood vessel endothelial cells by modulating the activity of nucleotide metabolism-regulating enzymes. This may contribute to the development of neurodegenerative processes.
CELLULAR AND MOLECULAR NEUROBIOLOGY
(2023)
Article
Chemistry, Medicinal
Paulina Mierzejewska, Noemi Di Marzo, Magdalena A. Zabielska-Kaczorowska, Iga Walczak, Ewa M. Slominska, Marialuisa Lavitrano, Roberto Giovannoni, Barbara Kutryb-Zajac, Ryszard T. Smolenski
Summary: The vascular endothelium is a crucial target for immune and thrombotic responses in xenotransplantation. This study evaluated the effects of simultaneous expression of three human genes on mouse endothelial function. The results showed that the simultaneous expression of these genes had beneficial effects on the biochemical and functional aspects of the endothelium, reducing inflammatory responses and improving endothelial permeability.
Correction
Biochemical Research Methods
Yong Wang, Suhua Yu, Rui Li, Jing Wan, Yuheng Wang, Zongli Huo, Chunyong Wu, Li Mi, Songqin Liu
JOURNAL OF CHROMATOGRAPHY A
(2024)
Review
Biochemical Research Methods
Israel D. Souza, Maria Eugenia C. Queiroz
Summary: Organic-silica hybrid monolithic materials have gained significant attention in separation science and sample preparation due to their unique properties. This review summarizes the characteristics, current status, and recent developments of these materials, as well as discusses synthesis methods, characterization techniques, and strategies for improving extraction efficiency in various applications.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Bhagwat Upreti, Bhanu Prasad Vempatapu, Jagdish Kumar, Pankaj K. Kanaujia
Summary: Lubricating oils are essential for the effective performance of internal combustion engines. This study presents a new HPLC method for determining additives in finished lubricating oils and polar substances in used engine oils.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zongqing Huang, Haoju Hua, Xiuzhen Du, Zipeng Zhen, Wei Zhao, Jun Feng, Ji-an Li
Summary: This study successfully isolated VHHs against the SUMO protein for the first time using biopanning of an immune camelid nanobody library. The VHHs were coupled to agarose resins to form affinity chromatography resins, allowing for one-step purification of various SUMO fusions.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Xueting Qiu, Xuben Hou, Yue Yang, Hao Fang, Fei Cui, Xinying Yang
Summary: In this study, a new method based on electrophoretically mediated microanalysis was developed for the investigation of PTPRO enzymatic activity. The method was successfully used to determine the kinetics of PTPRO and the inhibitory concentration of PTPRO inhibitors. The method exhibited improved accuracy and reduced consumption compared to traditional methods.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Feifei Luo, Mengmeng Hao, Lei Zhang, Yangguo Xie, Wei Hou, Hongya Wang, Zhongli Zhang
Summary: In this study, a comprehensive screening and structure elucidation of 23 nonvolatile organic compounds (NVOCs) in two antibody drugs was conducted. Seven previously unreported compounds were identified. A quantitative method with high sensitivity, accuracy, and precision was developed for evaluating the safety of NVOCs in drug products.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zuzana Vosahlova, Kveta Kalfkova, Martin Gilar, Jakub Szymarek, Maria Mazurkiewicz-Beldzinska, Sylwia Studzi
Summary: A hydrophilic interaction liquid chromatography method was developed for the separation and identification of oligonucleotide impurities and metabolites, and successfully applied to the analysis of serum samples from Spinraza-treated patients.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Aswanidevi Kongasseri, Thirumalai Madhesan, Sangeetha Krishna Kumar, Sushmitha Pedugu Sivaraman, Suchashrita Mitra, Pitchaiah Kancharlapalli Chinaraga, C. V. S. Brahmmananda Rao, Sivaraman Nagarajan, Prabhakaran Deivasigamani, Akhila Maheswari Mohan
Summary: This study focuses on the sequential separation of trivalent lanthanides using modified C-18 silica-packed supports and reversed-phase HPLC technique. By tuning the column modifiers and separation parameters, the researchers achieved systematic separation of Ln(3+). The results showed that the choice of column modifiers and fine-tuning of the modification procedure were crucial for achieving excellent separation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Raquel Perez-Robles, Szabolcs Fekete, Robert Kormany, Natalia Navas, Davy Guillarme
Summary: There is a mismatch between the sample diluent and the mobile phase in HILIC analysis, leading to analyte breakthrough and peak distortion. Adding a weak solvent for pre-injection can effectively prevent these issues and improve sensitivity.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oleg Pokrovskiy, Irina Rostovschikova, Konstantin Ustinovich, Ilya Voronov, Dmitry Kosyakov
Summary: A chromatography-like propagation of water through the material bed was observed during supercritical fluid extraction of aroma plants with high moisture content, which could be significant for modeling the kinetics of supercritical fluid extraction of non-volatile compounds.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Yasar Kemal Recepoglu, Ozguer Arar, Asli Yuksel
Summary: Biosorption using phosphorylated hazelnut shell waste (FHS) is a promising method for lithium removal and recovery. This study investigated the performance of a continuous-flow packed-bed column system using FHS for ultra-low level lithium removal. The results showed that the lihium biosorption capacity of the FHS column was unaffected by the bed height, but decreased with increasing flow rate.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Jian Sun, Zheheng Zheng, Zixiao Jia, Jiabin Wang, Xucong Lin
Summary: In this study, an ionic covalent organic framework (iCOF) surface grafting monolithic sorbent was prepared for in-tube solid-phase microextraction (SPME) of aristolochic acids (AAs) in various samples. The density of quaternary ammonium ions in the iCOF was modulated for enhanced adsorption of AAs. The iCOF surface grafting monolithic sorbent was confirmed by different means. Several operating conditions were optimized to develop the online in-tube SPME-HPLC method, which showed good linearity, low limits of detection, and high recoveries for AAs in serum samples. This study presents a feasible approach for the application of COF-based monolithic sorbents in sample preparation.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Zeng Wen, Ruihan Peng, Die Gao, Jing Lin, Jia Zeng, Zhou Li, Famin Ke, Zhining Xia, Dandan Wang
Summary: A chitosan-alginate sponge with multiple cross-linking networks was developed for adsorption and enrichment of anionic dyes from food samples. The sponge showed better three-dimensional network structure, mechanical behavior, and stability compared to pure chitosan and alginate sponges. The adsorption performance and selectivity of the sponge were systematically studied, and it maintained high adsorption capacity and selectivity for anionic dyes. The sponge also showed satisfactory reusability and was successfully combined with high-performance liquid chromatography for the enrichment and detection of anionic dyes in candy and cocktail samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Hairong Zhang, Xiaojuan Jiang, Dandan Zhang, Yuexin Yang, Qiang Xie, Caisheng Wu
Summary: Analysis of in vivo exposure to traditional Chinese medicine using mass spectrometry is helpful for identifying effective ingredients and developing new drugs. This study focused on Forsythia suspensa (Lian Qiao) and used metabolomics technology to explore its in vivo exposure. By comparing the differences before and after administration, a complete set of nontargeted analysis strategies for TCM exposure was established. The study identified 393 Lian Qiao-related chemical components in mice after administration, including prototypes and metabolites. This study provides a reference for research on the active ingredients of Lian Qiao in vivo.
JOURNAL OF CHROMATOGRAPHY A
(2024)
Article
Biochemical Research Methods
Oscar Castejon-Musulen, Ricardo Lopez, Ignacio Ontan, Vicente Ferreira
Summary: A quantitative analytical method was developed and validated in this study to determine the concentrations of 81 aroma-relevant wine volatiles. The method utilizes stir bar sorptive extraction and multidimensional gas chromatography with flame ionization detector or mass spectrometer. The method demonstrated good linearity, reproducibility, and recovery, and was successfully applied to analyze 20 wine samples.
JOURNAL OF CHROMATOGRAPHY A
(2024)