Review
Food Science & Technology
Zai-Bin Xie, Kai-Zheng Zhang, Zhen-Hui Kang, Jian-Gang Yang
Summary: Saccharomycopsis fibuligera, also known as Endomyces fibuliger or Saccharomyces fibuligera, is a yeast with high industrial application value due to its ability to produce ascospores and various enzymes. It has a high starch conversion capacity and plays a significant role in improving liquor quality through fermentation.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Yongtao Zhang, Dongdong Wang, Hao Fu, Dan Zhao, Jiachan Zhang, Meng Li, Changtao Wang
Summary: This study analyzes the extracellular proteins of Saccharomycopsis fibuligera and explores their significance in cosmetics. The findings suggest that these proteins can enhance antioxidant enzyme activity within cells, protect skin fibroblasts from oxidative stress, and delay skin aging.
JOURNAL OF FUNCTIONAL FOODS
(2022)
Article
Microbiology
Mohamed El-Agamy Farh, Najib Abdellaoui, Jeong-Ah Seo
Summary: This study found that under acidic pH conditions, Saccharomycopsis fibuligera undergoes a transition from yeast cells to hyphae, with a gradual decrease in carbohydrate-hydrolyzing enzyme activities. Additionally, significant changes in gene expression related to protein synthesis and signal transduction pathways were observed under acidic conditions.
FRONTIERS IN MICROBIOLOGY
(2021)
Article
Microbiology
Yvonne Methner, Frederico Magalhaes, Luis Raihofer, Martin Zarnkow, Fritz Jacob, Mathias Hutzler
Summary: The use of non-Saccharomyces yeast in beer brewing can produce unique flavors, with single cell isolates showing improved fermentation properties for industrial application.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Engineering, Chemical
Jose M. Vicaria, Otilia Herrera-Marquez, Mireya Serrano-Haro, Anais Vidal, Encarnacion Jurado, Jose L. Jimenez-Perez
Summary: The study found that specific surfactant formulations can enhance the stability of protease while reducing the deactivation of amylase. Nonionic surfactants play a role in stabilizing enzymes by interacting harmlessly with enzymes, avoiding competition with anionic surfactants at the same binding sites.
CHEMICAL ENGINEERING SCIENCE
(2022)
Article
Microbiology
Hye Yun Moon, Hyeon Jin Kim, Ki Seung Kim, Su Jin Yoo, Dong Wook Lee, Hee Je Shin, Jeong-Ah Seo, Hyun Ah Kang
Summary: The study identified 12 putative AATase genes in the genome of Saccharomycopsis fibuligera, showing differences in sequence with Saccharomyces cerevisiae Atf1p. Experimental results confirmed that the substrate preferences of SfAtf proteins were distinct from that of S. cerevisiae Atf1p.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Tae Hyeon Park, Chang-Yun Choi, Hyeon Jin Kim, Jeong-Rok Song, Damee Park, Hyun Ah Kang, Tae-Jip Kim
Summary: Two genes encoding probable alpha-L-arabinofuranosidase isozymes were discovered from the amylolytic yeast Saccharomycopsis fibuligera KJJ81, showing high amino acid sequence similarity. These isozymes displayed significant hydrolytic activity towards arabinoxylooligosaccharides and arabino-oligosaccharides, producing L-arabinose under specific conditions.
JOURNAL OF MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Microbiology
Ju-Wei Wang, Pei-Jie Han, Da-Yong Han, Sen Zhou, Kuan Li, Peng-Yu He, Pan Zhen, Hui-Xin Yu, Zhen-Rong Liang, Xue-Wei Wang, Feng-Yan Bai
Summary: The genetic diversity of the amylolytic yeast Saccharomycopsis fibuligera in Chinese liquor is largely unknown, but a study sequencing the genomes of 97 strains revealed four main lineages with varying levels of homozygosity and heterozygosity. The homozygous strains had small genomes and low sequence diversity, while the heterozygous strains exhibited larger genomes and higher sequence diversity. Some strains were found to be hybrids, with genomes containing sequences from an unidentified source.
JOURNAL OF MICROBIOLOGY
(2021)
Article
Biotechnology & Applied Microbiology
Paola Cremonesi, Cristiana Garofalo, Claudia Picozzi, Bianca Castiglioni, Nicola Mangieri, Vesna Milanovic, Andrea Osimani, Lucia Aquilanti
Summary: High sensitive qPCR and ddPCR assays were developed for quantifying total eumycetes and specifically determining contamination levels of spoilage yeasts in bread. The assays demonstrated strong performance in early detection and monitoring of bread quality through gene selection, specificity testing, sensitivity definition, and validation in artificially contaminated samples.
Article
Microbiology
Wanlin Liu, Xiaoye Luo, Shuyi Qiu, Wu Huang, Yanan Su, Linling Li
Summary: The study used untargeted metabolomics of liquid chromatography-mass spectrometry (LC-MS) and headspace solid-phase microextraction-gas chromatography (HS-SPME-GC-MS) to investigate the changes in volatile components and metabolites of Dendrobium officinale (D. officinale) juice fermented with starter cultures containing Saccharomycopsis fibuligera and Lactobacillus paracasei. The results showed that the co-culture of L. paracasei + S. fibuligera promoted the production of ethyl L (-)-lactate and enriched the flavor composition of D. officinale juice. Furthermore, several metabolic pathways were significantly up-regulated in the co-culture group, facilitating the synthesis of bioactive substances and enhancing the flavor harmonization of the fermented product.
Article
Multidisciplinary Sciences
Eun-Hee Park, Myoung-Dong Kim
Summary: Isolate Saccharomycopsis fibuligera MBY1320, from Korean traditional rice wine starter Nuruk, shows thermotolerance and starch-degrading activities. Its draft genome sequence has been deposited in public databases, showing similarity to other strains of the same species.
Article
Food Science & Technology
Miria B. D. Berteli, Olavo B. Q. Oliveira Filho, Janyeli D. S. Freitas, Wanessa C. Bortolucci, Gustavo R. Silva, Zilda C. Gazim, Francislaine A. R. Livero, Evellyn C. W. Lovato, Juliana S. Silveira, Giani A. Linde, Lilian Barros, Filipa S. Reis, Isabel C. F. R. Ferreira, Luzia D. Paccola-Meirelles, Nelson B. Colauto
Summary: The study determined and evaluated the chemical composition, cytotoxicity, and antioxidant activity of L. crinitus pileus and stipe separately, revealing differences in nutritional composition and active substances between them.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2021)
Article
Food Science & Technology
Ruiping Xu, Jinquan Han, Tie Tian, Nengbin Zhu, Yiling Chen, Eakapol Wangkahart, Zhiqi Zhang, Jun Liu, Denghang Yu, Jin Wei, Hongsen Xu
Summary: The present research investigated the effects of solid-state fermentation (SSF) with Lactobacillus plantarum and Saccharomycopsis fibuligera on the nutritional values and antioxidant activities of faba bean meal (FBM). The results showed that SSF with the two microorganisms effectively decreased tannin and phytic acid contents in FBM, and improved its nutritional quality and antioxidant capacity. L. plantarum was more effective in degrading tannins and increasing protein digestibility, while S. fibuligera was more effective in improving antioxidant activities and the amounts of phenol and flavonoid.
INTERNATIONAL JOURNAL OF FOOD SCIENCE AND TECHNOLOGY
(2023)
Article
Chemistry, Multidisciplinary
Pan Wang, Bingqin Li, Baiyue Li, Jing Yang, Xingran Xu, Shang-Tian Yang, Xiang Zou
Summary: This study investigated the carbon-economic biosynthesis of poly-2-hydrobutanedioic acid (P2HBD) from ethanol as the sole carbon source. The results showed that ethanol could be efficiently converted into P2HBD via a biosynthetic mechanism. A modular assembly strategy was designed to balance ethanol oxidation, glyoxylic acid shunt, and the gluconeogenesis pathway, and promoter engineering was used for precise regulation. An adaptive evolution strategy was performed to enhance ethanol tolerance, resulting in a mutant strain with comparable yields. These findings provide new insights into carbon-economic transformation from ethanol substrates into biopolymers and chemicals.
Article
Microbiology
Yinfeng Li, Guilan Jiang, Hua Long, Yifa Liao, Liuliu Wu, Wenyue Huang, Xiaozhu Liu
Summary: This study investigated the effects of trehalose supplementation on the survival, gene expression, cellular morphology, and oxidative stress tolerance of Wickerhamomyces anomalus under ethanol stress. The results showed that trehalose improved the survival rate and anomalous morphology of the yeast, as well as enhanced redox homeostasis and the activities of antioxidant enzymes. Trehalose also affected the expression of genes involved in various pathways.
Article
Nanoscience & Nanotechnology
Yudong Cao, Bin Chen, Haibin Zhong, Liyuan Pei, Guanglei Liu, Zhenglong Xu, Jianfeng Shen, Mingxin Ye
Summary: This study reports the design of a flexible sensor using Ti2C3Tx/polyurethane composites prepared by gas-liquid interface self-assembly. The sensor has a wide detection range, low-stress detection limit, high sensitivity, and fast response time, making it suitable for various applications such as wearable motion monitoring, underwater human motion detection, and monitoring of fish motion in water.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Review
Biotechnology & Applied Microbiology
Zhe Chi, Cun-Cui Kong, Zhuang-Zhuang Wang, Zhu Wang, Guang-Lei Liu, Zhong Hu, Zhen-Ming Chi
Summary: Aureobasidium spp. can adapt to different environments and regulate substrate utilization and cell growth through multiple signaling pathways. However, the molecular mechanisms of high temperature growth and glucose derepression in some novel strains of A. melanogenum are still unclear.
BIOTECHNOLOGY ADVANCES
(2022)
Article
Engineering, Chemical
Wei Yao, Xuanyang Li, Xiaodong Zhu, Liyuan Pei, Guanglei Liu, Yang Cheng, Mason Oliver Lam Chee, Pei Dong, Jianfeng Shen, Mingxin Ye
Summary: This study proposes a new method using nano carbon black composite aerogel for stable and efficient solar desalination. The composite material with inclined channels and hydrophilic/hydrophobic interface improves evaporation efficiency, stabilizes water output, and shows salt-resistant property.
Article
Biochemistry & Molecular Biology
Cong-Yan Qi, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: The research found that Aureobasidium melanogenum OUC and its engineered mutants can produce high molecular weight PMA, with the PMA yield of transgenic strain Crz46 increased by about twice compared to the native strain.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Materials Science, Multidisciplinary
Shun Guo, Wang Ding, Huihui Zhang, Weiqun Lu, Guanglei Liu, Haixia Liu, Xiaonong Cheng, Xinqing Zhao
Summary: In this study, a Ti-Nb alloy with large near-linear recoverable strain was designed to investigate the physical mechanisms responsible for high near-linear elastic deformability. The experimental results showed that the high near-linear elastic deformability of this alloy is attributed to a combined effect of large inherent elastic deformation and a reversible SIMT.
MATERIALS CHARACTERIZATION
(2022)
Review
Biochemistry & Molecular Biology
Peng Wang, Shu-Lei Jia, Guang-Lei Liu, Zhe Chi, Zhen-Ming Chi
Summary: Aureobasidium spp. are dimorphic fungi with both yeast and filamentous cells. They can synthesize various enzymes and natural products, and their genomes have been sequenced and annotated. They are considered as emerging cells for fungal biotechnology and biology.
PROCESS BIOCHEMISTRY
(2022)
Article
Biotechnology & Applied Microbiology
Zhu Wang, Mei Zhangna, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: This study successfully produced a high yield of liamocins through gene mutation and overexpression. The overexpressed genes promoted liamocins biosynthesis and sugar transport. Massoia lactone prepared from liamocins showed promising antifungal activity.
WORLD JOURNAL OF MICROBIOLOGY & BIOTECHNOLOGY
(2022)
Article
Engineering, Marine
Li Yuan, Hong-Qian Zhang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: In this study, it was found that stable nanemulsions loaded with Massoia lactone could be prepared using specific ingredients. The obtained nanemulsions showed high anti-fungal and free radical scavenging activities.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2022)
Article
Biotechnology & Applied Microbiology
Xin Wei, Miao Zhang, Guang-Yuan Wang, Guang-Lei Liu, Zhen-Ming Chi, Zhe Chi
Summary: In this study, engineered strains of A. pullulans var. aubasidani were found to be suitable for green fumaric acid production. The ornithine-urea cycle was identified as the key pathway for high level fumarate biosynthesis. Genetic modifications and overexpression led to the development of a strain that produced high yields of fumarate. This research is significant for global sustainable development and carbon neutrality.
SYNTHETIC AND SYSTEMS BIOTECHNOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Xin Wei, Miao Zhang, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: C-18:2 fatty acids play an important role in the cold and warm growth of psychrotrophic yeast, while C-18:3 fatty acids have no significant effect on cell growth. Additionally, the absence of C-18:2 fatty acids negatively affects cell wall development.
MARINE BIOTECHNOLOGY
(2023)
Article
Materials Science, Multidisciplinary
Bin Chen, Guanglei Liu, Minying Wu, Yudong Cao, Haibin Zhong, Jianfeng Shen, Mingxin Ye
Summary: Currently, there is a growing interest in the development of organohydrogel-based wearable flexible electronics (WFEs) due to their superior environmental stability compared to hydrogel-based WFEs. In this study, an efficient strategy is proposed to achieve the quick preparation of organohydrogels by utilizing the high reactivity of eutectic gallium-indium alloy and constructing a glycerol/water binary solvent system. The resulting organohydrogels, named liquid metal-based ion-conducting organohydrogels (LMIOs), demonstrate excellent environmental adaptability, high transparency, and remarkable mechanical properties and self-healing abilities, making them suitable for sensing human motions and applications in skin-attachable devices, robotics, and prosthetics.
ADVANCED MATERIALS TECHNOLOGIES
(2023)
Article
Chemistry, Physical
Guang-lei Liu, Wen-chao Xue, Yu-hao Cao, Xiao-xuan Sun, Zhi-qiang Li, Fu-hai Xu, Hai-xia Liu, Jian-zhong Zhou
Summary: This study investigates the effects of laser shock processing (LSP) on the thermal fatigue life and crack growth behavior of the coating and bonding area of the brake disc. The results show that LSP can enhance the thermal fatigue performance of the brake disc repaired by laser cladding.
JOURNAL OF ALLOYS AND COMPOUNDS
(2023)
Article
Materials Science, Multidisciplinary
Guanglei Liu, Wenchao Xue, Yuhao Cao, Zhiqiang Li, Xiaoxuan Sun, Fuhai Xu, Haixia Liu, Jianzhong Zhou
Summary: The effects of Laser Shock Peening (LSP) on the microhardness, residual stress, and microstructure of the Ni60 cladding layer and the combined area of the 20CrNiMo alloy were investigated. The results show that LSP can improve the microhardness, introduce residual compressive stress, and refine the microstructure. The residual compressive stress value is -538 MPa on the surface and -30 MPa at a depth of 2 mm.
JOURNAL OF LASER APPLICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Hao Chen, Cun-Cui Kong, Xin Wei, Zhe Chi, Guang-Lei Liu, Zhen-Ming Chi
Summary: In this study, an engineered strain of Aureobasidium melanogenum DFAK-ArgB6 overexpressing the ArgB gene was able to produce 3.0 g/L of L-piperazic acid from glucose during fed-batch fermentation. The yield was 55.6 mg L-piperazic acid/g glucose and the productivity was 24.8 mg/L/h, which is the highest reported so far. The partially purified L-piperazic acid had a purity of 86.4%.
PROCESS BIOCHEMISTRY
(2023)
Article
Marine & Freshwater Biology
Guanshuo Yu, Xiaoxiang Wang, Chao Zhang, Zhe Chi, Zhenming Chi, Guanglei Liu
Summary: In this study, a mangrove yeast strain Moesziomyces aphidis XM01 was used for efficient extracellular production of mannosylerythritol lipids (MELs). The produced MELs showed excellent physicochemical properties and various biological functions. The MELs were further self-assembled into nanomicelles with good stability and antibacterial activity. This research provides insights into efficient MEL production and broad application prospects of MEL nanomicelles.
MARINE LIFE SCIENCE & TECHNOLOGY
(2022)
Review
Biotechnology & Applied Microbiology
Zhi-Min Zhao, Zhi-Hua Liu, Tongtong Zhang, Rongqian Meng, Zhiqun Gong, Yibing Li, Jing Hu, Arthur J. Ragauskas, Bing-Zhi Li, Ying-Jin Yuan
Summary: Bioconversion of bioresources/wastes using Rhodococcus is a promising approach for producing lipid-derived bioproducts. However, bioconversion involves living cells and is a complex system that requires further understanding. This review highlights the potential of Rhodococcus for producing lipids from various wastes and presents genetic modifications using emerging tools for promoting bioconversion. Additionally, strategies for enhancing the bioaccessibility of lignin and its derived aromatics and synthesizing biodiesel and aviation fuels from lignin and lipids are discussed. The synergistic modification of microbial strains, substrates, and fermentation processes is proposed for sustainable biological lignin valorization.
BIOTECHNOLOGY ADVANCES
(2024)
Review
Biotechnology & Applied Microbiology
Maddy L. Newby, Joel D. Allen, Max Crispin
Summary: A key aspect of successful viral vaccine design is the elicitation of neutralizing antibodies targeting viral attachment and fusion glycoproteins. Glycosylation can influence the antigenicity and immunogenicity of vaccines, with native-like glycosylation potentially enhancing immunogenicity. Understanding viral glycosylation can guide the development of future vaccines in various enveloped viruses.
BIOTECHNOLOGY ADVANCES
(2024)
Review
Biotechnology & Applied Microbiology
Uganeeswary Suparmaniam, Man Kee Lam, Jun Wei Lim, Inn Shi Tan, Bridgid Lai Fui Chin, Siew Hoong Shuit, Steven Lim, Yean Ling Pang, Peck Loo Kiew
Summary: Microalgae have the ability to synthesize high-value phytochemicals, and subjecting them to abiotic stress can enhance the accumulation of valuable metabolites within their cells. This study explores the effects of manipulating abiotic stress on microalgae yields and discusses approaches to increase HVPCs production using abiotic stress.
BIOTECHNOLOGY ADVANCES
(2024)
Review
Biotechnology & Applied Microbiology
Pei Zhou, Cong Gao, Wei Song, Wanqing Wei, Jing Wu, Liming Liu, Xiulai Chen
Summary: The engineering status of protein is crucial for the efficient bioproduction in microbial cell factories. Strategies such as protein engineering, protein modification, and protein assembly can effectively modify the protein status and enhance production efficiency.
BIOTECHNOLOGY ADVANCES
(2024)
