期刊
BIOTECHNOLOGY & BIOTECHNOLOGICAL EQUIPMENT
卷 26, 期 6, 页码 3345-3350出版社
TAYLOR & FRANCIS LTD
DOI: 10.5504/BBEQ.2012.0107
关键词
Cladosporium cladosporioides; laccase; ABTS; purification
Cladosporium cladosporioides was identified as a laccase producer when grown on malt extract media supplemented with 0.02% ABTS (2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid). Extracellular laccase was purified to homogeneity from culture supernatant of Cladosporium cladosporioides by acetone precipitation, gel filtration chromatography and anion exchange chromatography, and was also characterized Purified laccase (specific activity 1785.71 U.mg(-1) is a monomeric protein with molecular weight of 50 kDa on SDS-PAGE. Purified laccase showed maximum activity at pH 5 and optimal temperature was observed 40 degrees C. The enzyme showed a high relative activity over a wide range of pH from 3 to 6 and was stable in a temperature range of 40 degrees to 70 degrees C. The K-m value of the purified enzyme was found to be 15 mu mol.L-1, while the Vmax value was observed to be 4612 mu mol.L-1.min(-1) with ABTS as a substrate. The catalytic activity of laccase was completely inhibited by dithiothreitol, sodium azide and L-cysteine at a final concentration of 5 mmol.L-1, and slightly inhibited by EDTA and SDS at a final concentration of 10 mmol.L-1. The laccase was activated by Cu2+ and Mg2+, and certain metal ions such as Ni+2, Ba+2 and Ag+2 inhibit the catalytic activity of enzyme at a final concentration of 1.0 mmol.L-1. To the best of our knowledge this is the first report on the purification and characterization of laccase from Cladosporium cladosporioides. Biotechnol. & Biotechnol. Eq. 2012, 26(6), 3345-
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