期刊
BIOTECHNIQUES
卷 48, 期 1, 页码 61-64出版社
FUTURE SCI LTD
DOI: 10.2144/000113304
关键词
protein solubilization; inclusion bodies; protein purification; sarkosyl; detergents
资金
- Oklahoma State University Agricultural Experimental Station [2527]
- NSF [0546506]
We describe a rapid, simple, and efficient method for recovering glutathione S-transferase (GST)- and His6-tagged maltose binding protein (MBP) fusion proteins from inclusion bodies. Incubation of inclusion bodies with 10% sarkosyl effectively solubilized >95% of proteins, while high-yield recovery of sarkosyl-solubilized fusion proteins was obtained with a specific ratio of Triton X-100 and CHAPS. We demonstrate for the first time that this combination of three detergents significantly improves binding efficiency of GST and GST fusion proteins to gluthathione (GSH) Sepharose.
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