期刊
BIOTECHNIQUES
卷 46, 期 2, 页码 130-132出版社
FUTURE SCI LTD
DOI: 10.2144/000113066
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资金
- European Union commission
- Japanese Society for the Promotion of Science
We present a high-throughput method for investigating the transcriptional starting sites of genes of interest, which we named Deep-RACE (Deep-rapid amplification of cDNA ends). Taking advantage of the latest sequencing technology, it allows the parallel analysis of multiple genes an is free of time-consuming cloning steps. In comparison to the sequencing of RACE PCR products, our approach is more precise and more cost-effective even for batches as small as 17.
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