Cytological detection of Wolbachia in squashed and paraffin embedded insect tissues

Wolbachia localization in situ is essential for accurate analysis of the infection and its consequences. Whole cell hybridization is proposed as an easy and rapid method for detecting Wolbachin cells in paraffin embedded tissues or testis squashes of Chorthippus parallelus (Orthoptera). Wolbachia is found in whole gonads and other adjacent tissues. A higher bacterial density, however, is observed in ovarioles and testis. Small independent bacteria with strictly cytoplasmic distribution are displayed. Bacterial density differences among individuals are also revealed.