Article
Cell Biology
Nazaret Gamez, Javiera Bravo-Alegria, Yumeng Huang, Nelson Perez-Urrutia, Deepa Dongarwar, Claudio Soto, Rodrigo Morales
Summary: Extensive experimental and human-derived evidence suggests that misfolded A beta particles spread similarly to infectious prions. Administration of A beta seeds accelerates brain amyloidosis. Injection of A beta seeds in the tongue accelerates the appearance of plaques, while extra-nasal exposure increases amyloid pathology in the olfactory bulb. The results suggest that A beta seeds can be transported to the brain by retrograde axonal transport, and research in this direction may be relevant in understanding disease mechanisms, diagnosis, and iatrogenic transmission risk evaluation.
Review
Chemistry, Analytical
Arezoo Mirzaie, Hassan Nasrollahpour, Balal Khalilzadeh, Ali Akbar Jamali, Raymond J. Spiteri, Hadi Youse, Ibrahim Isildak, Reza Rahbarghazi
Summary: Alzheimer's disease is a prevalent neurodegenerative disorder characterized by progressive deterioration, loss of neurons, and cognitive decline. Amyloid beta and Tau proteins are key biomarkers of the disease, and their abnormal levels in biofluids, particularly cerebrospinal fluid, are directly linked to the incidence and progression of Alzheimer's. Developing sensitive and reliable methods for screening and evaluating these biomarkers in cerebrospinal fluid can aid in early diagnosis and treatment. This article discusses the impact of Alzheimer's on cerebrospinal fluid and provides an overview of the latest biosensors for detecting amyloid beta and Tau in cerebrospinal fluid samples.
TRAC-TRENDS IN ANALYTICAL CHEMISTRY
(2023)
Review
Biochemistry & Molecular Biology
Diane L. Ritchie, Marcelo A. Barria
Summary: Accumulation and propagation of misfolded proteins in the brain are shared pathological features of many neurodegenerative diseases. While there is no epidemiological evidence suggesting infectiousness in neurodegenerative disorders, experimental models show potential prion-like transmission of other pathogenic proteins. Concerns exist regarding the transmission of misfolded proteins beyond prion protein.
Article
Biochemistry & Molecular Biology
Dilek Nur Altay, Hulya Yagar, Hakki Mevlut Ozcan
Summary: A novel label-free impedimetric immunosensor was developed for quantitative analysis of Aβ42 protein in Alzheimer's disease diagnosis. The immunosensor was fabricated using inexpensive and disposable ITO-PET electrodes and the specific antibody was immobilized after electrode modification. The interaction between anti-Aβ42 and Aβ42 was analyzed using EIS and CV techniques. The immunosensor showed a linear detection range of 1-100 pg/mL and a limit of detection of 0.37 pg/mL. The biosensor's reproducibility, repeatability, storage stability, selectivity, and regeneration were investigated. The kinetic behavior of antibody-antigen complex formation was determined using SFI analysis.
BIOELECTROCHEMISTRY
(2023)
Article
Neurosciences
Suzanne Lam, Fanny Petit, Anne-Sophie Herard, Susana Boluda, Sabiha Eddarkaoui, Martine Guillermier, Luc Buee, Charles Duyckaerts, Stephane Haik, Jean-Luc Picq, Marc Dhenain
Summary: The transmission of tau pathology by human brain extracts inoculations in primates has been experimentally demonstrated for the first time. Widespread A beta and tau pathologies can be associated with cognitive decline. The study highlights the importance of systematic monitoring for individuals at risk of iatrogenic transmission of A beta and tau.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Neurosciences
Alejandro Ruiz-Riquelme, Alison Mao, Marim M. Barghash, Heather H. C. Lau, Erica Stuart, Gabor G. Kovacs, K. Peter R. Nilsson, Paul E. Fraser, Gerold Schmitt-Ulms, Joel C. Watts
Summary: Aggregates containing longer Aβ C-terminal variants are more potent inducers of cerebral Aβ deposition. Aβ43 seeds may play a crucial role in the initial stages of Aβ pathology in AD.
ACTA NEUROPATHOLOGICA COMMUNICATIONS
(2021)
Article
Biochemistry & Molecular Biology
Guilian Xu, Susan Fromholt, David R. Borchelt
Summary: The amyloid pathology features of Alzheimer's disease can be classified into fibrillary and diffuse. Studies suggest that different populations of misfolded A beta conformers compete to populate the brain.
Review
Chemistry, Medicinal
Weimin Qiu, Hui Liu, Yijun Liu, Xin Lu, Lei Wang, Yanyu Hu, Feng Feng, Qi Li, Haopeng Sun
Summary: Alzheimer's disease (AD) is a difficult to treat progressive neurodegenerative disease characterized by the accumulation of amyloid beta (A beta) plaques in the brain. A beta interacts with various receptors on the plasma membrane and mediates signaling pathways that contribute to the development of AD. Despite ongoing research, there are currently no effective medications for AD. This review discusses the importance of A beta in the pathogenesis of AD, recent progress in targeting A beta-related receptors and compounds, and the challenges and opportunities in developing effective therapies for AD.
MEDICINAL RESEARCH REVIEWS
(2023)
Article
Biochemistry & Molecular Biology
Kaori Tsukakoshi, Rikako Kubo, Kazunori Ikebukuro
Summary: In this study, a fusion protein called PrP-ALP was developed as a sensing element to detect toxic A beta oligomers. PrP-ALP showed strong affinity towards high molecular weight oligomers and has potential for detecting A beta oligomers. This study is important for understanding the pathogenesis of Alzheimer's disease.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Takuto Shimizu, Emiko Nogami, Yuka Ito, Kazuo Morikawa, Masaki Nagane, Tadashi Yamashita, Tsuyoshi Ogawa, Fuyuki Kametani, Hisashi Yagi, Naomi Hachiya
Summary: Prion disease is a neurodegenerative disorder with no effective therapeutic agents developed so far.
NEUROCHEMICAL RESEARCH
(2021)
Review
Electrochemistry
Yun-Xiao Feng, Chang-Dong Chen
Summary: This review mainly highlights the development of nanomaterial-based electrochemical biosensors for the early diagnosis of Alzheimer's disease (AD) and discusses the comprehensive utilization of nanomaterials as electrode substrates and signal reporters.
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2022)
Review
Biochemistry & Molecular Biology
Anne-Cathrine S. Vogt, Gary T. Jennings, Mona O. Mohsen, Monique Vogel, Martin F. Bachmann
Summary: Alzheimer's disease is the most common form of dementia and is responsible for 60-70% of cases. The number of people with dementia is expected to triple by 2050 due to an aging population. Currently, there are only symptomatic treatments available, making it crucial to develop novel therapeutic strategies to prevent or delay the onset of Alzheimer's disease. This mini-review focuses on the understanding of Alzheimer's disease pathobiology and discusses current immunomodulating therapies targeting amyloid-beta protein.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biotechnology & Applied Microbiology
Akiko Amano, Nobuo Sanjo, Wataru Araki, Yasutaka Anraku, Makoto Nakakido, Etsuro Matsubara, Takami Tomiyama, Tetsuya Nagata, Kouhei Tsumoto, Kazunori Kataoka, Takanori Yokota
Summary: Researchers developed polymeric nanomicelles capable of passing through the blood-brain barrier to deliver specific antibodies to the brain. The administration of 6H4 antibody fragments in polymeric nanomicelles for 10 weeks significantly reduced the amounts of toxic A beta species in the brain and inhibited the formation of A beta plaques. Behavioral analysis also showed an improvement in spatial reference memory in mice with Alzheimer's disease.
JOURNAL OF NANOBIOTECHNOLOGY
(2023)
Article
Clinical Neurology
Lei Liu, Hyunchang Kwak, Trebor L. Lawton, Shan-Xue Jin, Angela L. Meunier, Yifan Dang, Beth Ostaszewski, Alison C. Pietras, Andrew M. Stern, Dennis J. Selkoe
Summary: The study developed a new method for detecting and quantifying soluble oligomers of amyloid beta protein in human plasma. The method is highly sensitive, cost-effective, and suitable for high throughput analysis.
ALZHEIMERS & DEMENTIA
(2022)
Review
Biochemistry & Molecular Biology
Lucia Gallego Villarejo, Lisa Bachmann, David Marks, Maite Brachthaeuser, Alexander Geidies, Thorsten Mueller
Summary: Intracellular amyloid beta (iAβ) plays a crucial role in neurodegeneration and serves as a pathological marker. Modulating iAβ through pharmacological treatment has shown beneficial effects on cognitive properties. Future research should focus on the impact of viral infections on iAβ generation.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Neurosciences
Barbara Kramarz, Rachael P. Huntley, Milagros Rodriguez-Lopez, Paola Roncaglia, Shirin C. C. Saverimuttu, Helen Parkinson, Rina Bandopadhyay, Maria-Jesus Martin, Sandra Orchard, Nigel M. Hooper, David Brough, Ruth C. Lovering
JOURNAL OF ALZHEIMERS DISEASE
(2020)
Article
Geriatrics & Gerontology
Ross A. Dunne, Dag Aarsland, John T. O'Brien, Clive Ballard, Sube Banerjee, Nick C. Fox, Jeremy D. Isaacs, Benjamin R. Underwood, Richard J. Perry, Dennis Chan, Tom Dening, Alan J. Thomas, Jeffrey Schryer, Anne-Marie Jones, Alison R. Evans, Charles Alessi, Elizabeth J. Coulthard, James Pickett, Peter Elton, Roy W. Jones, Susan Mitchell, Nigel Hooper, Chris Kalafatis, Jill G. C. Rasmussen, Helen Martin, Jonathan M. Schott, Alistair Burns
Summary: MCI is a clinically heterogeneous syndrome that can lead to further cognitive decline, but may also remain stable or resolve over time. Diagnostic criteria, including cognitive testing, neuroimaging, and fluid biomarkers, can improve accuracy of diagnosis and potentially guide prognosis. Large-scale studies are needed to determine the practical usefulness of combining multiple markers for clinical use.
Article
Biochemistry & Molecular Biology
Rahagir Salekeen, Asif Ahmed, Md Emdadul Islam, Md Morsaline Billah, Hafizur Rahman, Kazi Mohammed Didarul Islam
Summary: This study explores the potential of therapeutic plant peptides for combating cellular senescence by targeting key proteins in the ageing network. Using computational methods, the researchers identified 25 phytopeptides with potential anti-ageing properties, and found that one specific peptide, Cycloleonuripeptide B, exhibited strong binding affinity with key proteins. This computational study lays the groundwork for future laboratory and clinical research into repurposing therapeutic phytopeptides for cellular senescence and related pathophysiology.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biochemistry & Molecular Biology
Rahagir Salekeen, Sadia Noor Mou, Md Emdadul Islam, Asif Ahmed, Md Morsaline Billah, S. M. Mahbubur Rahman, Kazi Mohammed Didarul Islam
Summary: This study is the first to use cheminformatic approaches to investigate the molecular linkage between the bioactive compounds of the mangrove plant Heritiera fomes and the arachidonic acid metabolic network. The findings suggest that H. fomes may serve as a functional reservoir of safe and potent natural anti-inflammatory compounds, and highlight two specific compounds as potential inhibitors of multiple catalytic checkpoints in the inflammatory network. This research provides a primary guideline for future testing of the anti-inflammatory potential of H. fomes as a source of drug-like molecules.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Review
Virology
Mohammad Mahmudur Rahman, Maruf Hasan, Asif Ahmed
Summary: This study discusses the mechanism of SARS-CoV-2 entering host cells, focusing on the role of sACE2 in viral infection, as well as the impact of Ang II and AT1R in disease severity in comorbid patients.
REVIEWS IN MEDICAL VIROLOGY
(2021)
Article
Geriatrics & Gerontology
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Stefano Patassini, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper
Summary: The study found alterations in levels of several metals across multiple regions of Parkinson's disease dementia (PDD) brain, with widespread decreases in copper being the most common, closely resembling those in Alzheimer's disease dementia (ADD) patients, suggesting similar disease mechanisms in both dementias.
FRONTIERS IN AGING NEUROSCIENCE
(2021)
Article
Chemistry, Multidisciplinary
Marilena Hadjidemetriou, Jack Rivers-Auty, Lana Papafilippou, James Eales, Katherine A. B. Kellett, Nigel M. Hooper, Catherine B. Lawrence, Kostas Kostarelos
Summary: Blood-circulating biomarkers have the potential to detect AD pathology early and improve clinical trial outcomes, but currently there are no validated blood-based biomarkers for AD. A nanoparticle-based proteomics enrichment approach was used to discover and track changes in the blood proteome in an AD mouse model, revealing multiple proteomic signals indicative of asymptomatic stages of AD. The potential of this nano-omics approach to enrich the identification of blood-circulating signals associated with neurodegeneration was demonstrated, emphasizing the importance of integrating nanotechnology with proteomic tools for predicting the progression of AD.
Review
Toxicology
Fahim Rejanur Tasin, Asif Ahmed, Debasish Halder, Chanchal Mandal
Summary: Epigenetic modifications by toxic heavy metals, such as lead, have been extensively studied in the field of genomic research. Lead exposure during pregnancy can cause abnormal fetal development through changes in DNA methylation, histone modifications, and microRNA expression. These modifications have been associated with a wide range of health issues, including impaired growth, obesity, autism, diabetes, cardiovascular diseases, cancer risks, and Alzheimer's disease.
JOURNAL OF APPLIED TOXICOLOGY
(2022)
Article
Neurosciences
Melissa Scholefield, Stephanie J. Church, Jingshu Xu, Stefano Patassini, Federico Roncaroli, Nigel M. Hooper, Richard D. Unwin, Garth J. S. Cooper
Summary: Elevated brain urea levels have been reported in age-related dementias such as Alzheimer's disease and Huntington's disease. This study found substantively elevated urea concentrations in all nine regions of the brain in Parkinson's disease dementia, indicating a common metabolic disturbance shared with Alzheimer's disease and Huntington's disease.
FRONTIERS IN MOLECULAR NEUROSCIENCE
(2021)
Review
Biochemistry & Molecular Biology
Shabana Cassambai, Hannah Bridge, Palak K. Gill, Similoluwa Shobaloju, Tiziana Sgamma, Jo Rushworth
Summary: This article presents a co-created, flipped feed-in approach to designing a more inclusive virtual biochemistry lab assessment. By replacing traditional feedback with peer marking and unexemplars, the approach effectively challenges and engages a large, diverse cohort of students, resulting in a high pass rate and student satisfaction. Furthermore, the approach has shown potential in reducing disability-based attainment gaps and warrants further investigation into reducing gaps based on ethnicity, gender, and age.
ESSAYS IN BIOCHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Katherine A. B. Kellett, Kate Fisher, Harry Aldworth, Nigel M. M. Hooper
Summary: Bone morphogenetic protein 1 (BMP1), a member of the astacin family of zinc-metalloproteases, cleaves LDL receptor (LDLR) within its ligand-binding domain, reducing LDL-cholesterol binding and uptake. Only BMP1, among the other astacin proteases, was found to contribute to the cleavage of LDLR. Mutations at the P1' and P2 positions of the cleavage site rendered mouse LDLR susceptible to BMP1 cleavage. This study provides insight into the mechanisms regulating LDLR function.
Article
Neurosciences
Wenjun Zhang, Xiangjun Zhao, Xuewei Qi, Susan J. Kimber, Nigel M. Hooper, Tao Wang
Summary: In this study, induced pluripotent stem cell (iPSC) models were established from CADASIL patients to investigate the abnormal neurovascular interaction and blood-brain barrier (BBB) function. The impaired capability of vascular mural cells in CADASIL patients and the decreased barrier function of blood-brain barrier endothelial cells were observed. These findings provide new insights into early disease pathologies and have implications for future therapeutic development.
FRONTIERS IN CELLULAR NEUROSCIENCE
(2023)
Review
Rheumatology
A. M. Snedden, K. A. B. Kellett, J. B. Lilleker, N. M. Hooper, H. Chinoy
Summary: This review discusses the pathological effects of protein aggregation and the process of aggregation following immune attack in inclusion body myositis (IBM). Immune attack is likely to cause protein aggregation by impairing endoplasmic reticulum and mitochondrial function. Aggregated proteins may activate inflammatory responses or provoke immune reactions. Protein aggregates may impair cellular mechanisms and propagate along myocytes, resulting in muscle weakness and atrophy.
CLINICAL AND EXPERIMENTAL RHEUMATOLOGY
(2022)
Article
Chemistry, Multidisciplinary
D. Merryweather, S. R. Moxon, A. J. Capel, N. M. Hooper, M. P. Lewis, P. Roach
Summary: Cellular metabolism and behaviour are closely linked to cytoskeletal tension and scaffold mechanics. In this study, a 3D culture system using diluted type I collagen hydrogel to produce variation in gel stiffness was explored. The results showed that hydrogel scaffold remodelling was linked to gel collagen concentration and different concentrations of gel led to significant changes in programmed cell behaviour and cell morphology.
Article
Public, Environmental & Occupational Health
Asif Ahmed, Tasnima Haque, Mohammad Mahmudur Rahman
FRONTIERS IN PUBLIC HEALTH
(2020)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)