期刊
BIOSENSORS & BIOELECTRONICS
卷 57, 期 -, 页码 284-291出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.02.009
关键词
Point-of-care; Autonomous; Capillary microfluidics; ELISA; Photodiodes
类别
资金
- Fundacao para a Ciencia e a Tecnologia (FCT) through the Projecto Estrategico [PEst-OE/CTM/LA0024/2013]
- Associated Laboratory IN - Institute of Nanoscience and Nanotechnology
- Fundação para a Ciência e a Tecnologia [PEst-OE/CTM/LA0024/2013] Funding Source: FCT
The miniaturization of biosensors using microfluidics has potential in enabling the development of point-ofcare devices, with the added advantages of reduced time and cost of analysis with limits-of-detection comparable to those obtained through traditional laboratory techniques. Interfacing microfluidic devices with the external world can be difficult especially in aspects involving fluid handling and the need for simple sample insertion that avoids special equipment or trained personnel. In this work we present a point-of-care prototype system by integrating capillary microfluidics with a microfabricated photodiode array and electronic instrumentation into a hand-held unit. The capillary microfluidic device is capable of autonomous and sequential fluid flow, including control of the average fluid velocity at any given point of the analysis. To demonstrate the functionality of the prototype, a model chemiluminescence ELISA was performed. The performance of the integrated optical detection in the point-of-care prototype is equal to that obtained with traditional bench-top instrumentation. The photodiode signals were acquired, displayed and processed by a simple graphical user interface using a computer connected to the microcontroller through USB. The prototype performed integrated chemiluminescence ELISA detection in about 15 min with a limit-ofdetection of approximate to 2 nM with an antibody-antigen affinity constant of approximate to 2 x 10(7) M-1. (c) 2014 Elsevier B.V. All rights reserved.
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