期刊
BIOSENSORS & BIOELECTRONICS
卷 60, 期 -, 页码 252-258出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.04.031
关键词
DNA; Peroxidase; Copper; Coordination; Biomimetics
类别
资金
- National Natural Science Foundation of China [21206107, 31200753]
- Program for Changjiang Scholars and Innovative Research Team in University [IRT1161]
Herein a facile method is proposed to construct DNA-based peroxidase mimetics simply assembled by polymorphic DNA and Cu2+ ions. The Cu(II)-catalyzed oxidation of TMB in the presence of H2O2 can be significantly accelerated through Cu(II)-coordination with DNA scaffolds, of which a colorimetric change can be discerned by naked-eye. The reaction rates of DNA-Cu(II) complexes are directly associated with sequence composition as well as the secondary structure of DNA scaffold, e.g., the reaction rate decreases in the following order: GpG-duplex approximate to G-rich coil > G-quadruplex > C-rich coil > i-motif. It is the first report to explore a colorimetric Cu2+ sensing system on the basis of peroxidase mimicking activities of polymorphic DNA-Cu(II) complexes. One of our most intriguing results is that the GpG-duplex DNA demonstrates the ability to sense Cu2+ ions in aqueous solution without significant interference from other metal ions. The Cu2+ detection limit of 1.2 nM is achieved with a linear response range of 1.2-100 nM, and the developed sensing system is potentially applicable for quantitative determination of Cu2+ in drinking water samples. (C) 2014 Elsevier B.V. All rights reserved.
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