Article
Chemistry, Multidisciplinary
Xiaowei Wei, Zehan Cai, Lijun Cai, Yu Wang, Dagan Zhang, Yefei Zhu
Summary: This article introduces a novel multiplex platform for efficient detection of bladder cancer-related microRNAs by integrating photonic crystal barcodes with branched hybridization chain reaction. The platform shows high sensitivity and accuracy, and the results are consistent with conventional detection methods. This method provides a new approach for the clinical diagnosis of bladder cancer.
ADVANCED MATERIALS INTERFACES
(2022)
Article
Chemistry, Analytical
Maha K. Rahim, Jinghui Zhao, Hinesh V. Patel, Hauna A. Lagouros, Rajesh Kota, Irma Fernandez, Enrico Gratton, Jered B. Haun
Summary: Fluorescence lifetime-based multiplexing allows simultaneous detection of multiple probes, and the phasor approach provides a simple solution for lifetime analysis. This study demonstrates the ability of phasor analysis to resolve and quantify exogenous fluorescent probes based on lifetime information alone. It also shows that lifetime-based quantitation accuracy can be improved through intensity matching. The results highlight the potential of fluorescence lifetime imaging and phasor analysis as a powerful tool for simultaneous detection of multiple biomarkers.
ANALYTICAL CHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Zhilun Zhao, Chen Chen, Shixuan Wei, Hanqing Xiong, Fanghao Hu, Yupeng Miao, Tianwei Jin, Wei Min
Summary: Raman-active nanoparticles (Rdots) with ultra-brightness and compact sizes are developed for high-resolution imaging of complex biological networks and immunostaining of specific protein targets.
NATURE COMMUNICATIONS
(2021)
Article
Chemistry, Multidisciplinary
Yu Jiang, Hua Chai, Ninghan Feng, Peng Miao
Summary: miRNAs are powerful biomarkers for various pathological processes, with miR-200c being a critical signature of prostate cancer. An established biosensor using electrochemical techniques and ligation-dependent cascade strand displacement amplification shows high sensitivity and reliability for miRNA detection. This sensor can discriminate target miRNAs from mismatched ones and performs well in biological samples, offering a potential application for miRNA sensing.
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2021)
Article
Chemistry, Multidisciplinary
Xiaobo Zhou, Lingfeng Zhao, Ke Zhang, Chaojie Yang, Shijie Li, Xiaoxia Kang, Guo Li, Qi Wang, Haiwei Ji, Mingmin Wu, Jinxia Liu, Yuling Qin, Li Wu
Summary: This study presented a strategy for designing and fabricating narrow emissive AIEdots with tunable wavelengths by constructing a light-harvesting system with high energy transfer efficiency, resulting in high brightness and narrow emission.
Review
Chemistry, Multidisciplinary
Hwapyong Kim, Ayeong Choe, Seung Beom Ha, Ghulam Mustafa Narejo, Sung Wook Koo, Ji Su Han, Wookjin Chung, Jae-Yup Kim, Jiwoong Yang, Su-Il In
Summary: This review mainly discusses the application of quantum dot-sensitized semiconductors in photoelectrochemical hydrogen production, focusing on the advancements in improving long-term stability through the use of cocatalysts and passivation layers. The working principle of PEC cells, important configurations, and equations for assessing PEC performance are also reviewed.
Article
Chemistry, Analytical
Yeling Yang, Jingzhao Yang, Yixia Yang, Xinghu Ji, Wenhua Li, Fubing Wang, Conghua Xie, Zhike He
Summary: We developed a novel detection system based on CRISPR-Cas-catalyzed formation of quantum dot-DNA (QD-DNA) hydrogel for sensitive miRNA assay. This assay, termed CRISPR-Cas-catalyzed TMSD based QD-DNA hydrogel (Cas-TMSD-QDH), showed an accurate detection of miR-17 levels in different cell lines and serum samples, revealing great potential for miRNAs assay in clinical molecular diagnostics.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Analytical
Wang Luo, Ting Wang, Zhi Weng, Shulian Bai, Li Zhang, You Wu, Hongyan Yu, Dan Bai, Ding Wang, Huajian Chen, Yongcan Guo, Yujun Yang, Junjie Li, Zhang Zhang, Guoming Xie
Summary: In this study, the researchers improved the selectivity and responsiveness of SNV identification by inserting bulge-loops in probes to fine-tune the thermodynamic changes of the entropy-driven catalytic reaction (EDC). They also used oligonucleotides with chromophores to create microspheres with different fluorescence characteristics, constructing a set of convenient and flexible barcodes. By coupling the bulge-loops tuned EDC probes and the Tag-encoded barcodes, they designed a biosensor that allowed simultaneous detection of low-abundance five-plexed EGFR mutations in the same system.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Vassiliki Katseli, Michailia Angelopoulou, Christos Kokkinos
Summary: This work describes a novel configuration of integrated all-3D-printed electrochemical microtitration wells for direct quantum dot-based and enzymatic bioassays. The e-wells enable the in situ development of complete bioassays without the need for external equipment. They demonstrate universal applicability in both voltammetric bioassays and amperometric determination of enzymatic biosensing.
ADVANCED FUNCTIONAL MATERIALS
(2021)
Article
Chemistry, Multidisciplinary
Alexander M. Saeboe, Alexey Y. Nikiforov, Reyhaneh Toufanian, Joshua C. Kays, Margaret Chern, J. Paolo Casas, Keyi Han, Andrei Piryatinski, Dennis Jones, Allison M. Dennis
Summary: This report showcases the reddest emitting indium phosphide quantum dots to date, demonstrating tunable near-infrared photoluminescence and PL multiplexing in the first optical tissue window while avoiding toxic constituents. By utilizing an inverted QD heterostructure, the synthesis overcomes the growth bottleneck and extends the emission peak of the nanoparticles into the deeper optical tissue window.
Article
Chemistry, Multidisciplinary
Wancun Zhang, Pin Zhang, Ying Liang, Weyland Cheng, Lifeng Li, Huanmin Wang, Zhidan Yu, Yan Liu, Xianwei Zhang
Summary: In this study, a ratiometric hairpin DNA probe based electrochemical biosensor was developed for rapid and sensitive detection of Hg2+. The biosensor showed high ultrasensitivity, specificity, repeatability, and reproducibility in quantifying trace Hg2+. It exhibited a broad linear dynamic range and high sensitivity, and demonstrated excellent selectivity towards Hg2+ ions in the presence of other metal ions. The biosensor has been successfully applied in the detection of Hg2+ in various samples with good recovery rate and small relative standard deviations.
Article
Chemistry, Analytical
Ruomeng Li, Fengzhe Li, Yanping Zhang, Yuqiu He, Yushi Wang, Fuan Wang
Summary: Accurate diagnosis requires the development of multiple guaranteed DNA circuits. We developed a compact-yet-efficient hierarchical DNA hybridization (HDH) circuit for simultaneous analysis of multiple miRNAs and specifically discriminating cancer cells. The HDH circuit enables programmable multiple microRNA activations and localized cascaded signal amplification. It provides a powerful toolkit for accurate diagnostics and pathological evolution.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Tae Won Nam, Yeonkyung Park, Yeon Sik Jung, Hyun Gyu Park
Summary: In this study, a polychromatic quantum dot array (PQDA) was developed for accurate quantification of multiple miRNAs. The PQDA, manufactured using advanced patterning techniques, showed a wide dynamic range and high specificity, enabling the identification of target miRNAs with high sensitivity.
Article
Chemistry, Analytical
Yunfei Guo, Jun Li, Hao Yang, Hongchen Gu, Gaolian Xu, Hong Xu
Summary: STEM-Mi-PCR is a multiplexed miRNA detection method that allows for simultaneous detection of multiple miRNAs with high sensitivity and specificity. It can also discriminate single nucleotide mutations in miRNAs. This method holds promise for future clinical applications in miRNA profiling.
Article
Chemistry, Analytical
Bo Zhang, Wan-Sheng Tang, Shou-Nian Ding
Summary: In this study, a simple simulation strategy based on a multicolor fluorescence model was used to predict the influence of microbead size distribution on barcode signals, simplifying the experimental iteration process and achieving good agreement between experimental clusters of fluorescent microbeads and simulated barcodes. When applied for high-throughput ultrasensitive detection of tumor markers, these barcodes exhibit superior detection performance, meeting the sensitivity criteria of tumor marker analysis.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)