Article
Spectroscopy
Yan Zhou, Zefeng Wang, Shengnan Zhang, Le Deng
Summary: By integrating the advantages of catalytic hairpin components and multi-foot DNA walker, a high-sensitivity detection method for pathogen bacteria has been designed. This method does not rely on enzymes or sophisticated instrumentation, making it a promising technique for pathogenic bacteria detection.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Article
Biotechnology & Applied Microbiology
N. E. Gasz, M. J. Geary, S. L. Doggett, M. L. Harvey
Summary: The blowfly species Lucilia sericata and Lucilia cuprina play roles as agricultural pests, in forensics, and in medicine. While L. sericata larvae are used in maggot debridement therapy, L. cuprina larvae are major initiators of sheep myiasis in certain regions. Bacterial DNA extraction and sequencing showed that digestive tracts and female salivary glands carried the most bacteria in both species, with genera Pseudomonas and Corynebacterium being highly represented. Further research could focus on key organs like spermathecae and salivary glands to understand the role of bacteria in the blowfly life cycle.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2021)
Article
Environmental Sciences
Weijiao Liu, Muhammad Nasir, Mengjie Yan, Xiangzhen Zhu, Muhammad Shahid Iqbal, Li Wang, Kaixin Zhang, Dongyang Li, Jichao Ji, Xueke Gao, Junyu Luo, Jinjie Cui
Summary: This study examined the effects of Cry1B protein on the growth and development of non-target natural enemies. The results showed that Cry1B protein had no significant impact on the health indicators of P. astrigera, but it did reduce the number of symbiotic bacteria species and species diversity. The effects of Cry1B protein on symbiotic bacteria varied by growth and development stage and sex.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Environmental Sciences
Keng Po Lai, Chau Fong Tsang, Lei Li, Richard Man Kit Yu, Richard Yuen Chong Kong
Summary: Microplastics in municipal sewage can harbor pathogenic bacteria, promoting their colonization and growth, posing potential threats to public health and aquatic ecosystems.
Article
Microbiology
Todd Testerman, Lidia Beka, Stephen R. Reichley, Stacy King, Timothy J. Welch, Gregory D. Wiens, Joerg Graf
Summary: Simultaneous microbial community profiling and pathogen detection were conducted in a rainbow trout aquaculture facility. The study found fish pathogens in multiple locations and observed differences in community composition based on surface types. The results provide insights into the microbial communities and pathogen distribution in aquaculture facilities.
FEMS MICROBIOLOGY ECOLOGY
(2022)
Article
Veterinary Sciences
Xiaohui Wen, Shengjun Luo, Dianhong Lv, Chunling Jia, Xiurong Zhou, Qi Zhai, Li Xi, Caijuan Yang
Summary: This study used high-throughput sequencing technology to investigate the gut microbiota of different horse breeds and found variations in microbial composition and function. Thoroughbred horses had a more diverse microbiota, while Hybrid horses showed intermediate diversity. The metabolic pathways also differed significantly between breeds.
FRONTIERS IN VETERINARY SCIENCE
(2022)
Article
Immunology
Qian Zhang, Shumin Wang, Xinyu Zhang, Kexin Zhang, Ying Li, Yansong Yin, Ruiling Zhang, Zhong Zhang
Summary: The gut microenvironment of houseflies plays a crucial role in their development and immune response. This study isolates and analyzes different bacterial strains from housefly larvae, and finds that the oral administration of various bacteria has differential effects on larval development and immunity. Beneficial bacteria can be used as probiotic additives for larvae feeding, while harmful bacteria could be targeted for pest control. Additionally, the study provides insights into the immune response of houseflies to external microorganism stimulation.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Chemistry, Analytical
Huseyin Oguzhan Kaya, Arif E. Cetin, Mostafa Azimzadeh, Seda Nur Topkaya
Summary: Detection of pathogens, such as bacteria and viruses, remains a challenge in analytical medicine, but utilizing electrochemical methods can achieve rapid, inexpensive, specific, and sensitive detection. Electrochemical biosensors have made significant advances in the detection of bacteria and viruses, showing a wide range of potential applications.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Environmental Sciences
Yongjing Chen, Guijia Liu, Maria Rafraf Ali, Mingzhu Zhang, Guowei Zhou, Qingye Sun, Mingjun Li, Jazbia Shirin
Summary: Soil cadmium (Cd) pollution poses a significant threat to flora and fauna due to its non-degradable and transferable nature. This study investigated the impact of endogenous Cd-polluted mulberry leaves on the gut microbiota of silkworms (Bombyx mori). The results revealed that Cd exposure caused significant changes in the gut bacterial composition of the silkworms, but had negligible effects on the phyllosphere bacteria of mulberry leaves. The findings suggest that the perturbations in the gut microbiota of B. mori are driven by Cd content rather than phyllosphere bacteria.
ECOTOXICOLOGY AND ENVIRONMENTAL SAFETY
(2023)
Article
Food Science & Technology
Anderson Carlos Camargo, Edite Andrade Costa, Andressa Fusieger, Rosangela de Freitas, Luis Augusto Nero, Antonio Fernandes de Carvalho
Summary: Minas Gerais is a Brazilian state known for being the largest cheese producer in Brazil, with Entre Serras region experiencing a resurgence in artisanal cheese production. The predominant bacterial species in Entre Serras MAC was found to be Lactococcus lactis, with some farms showing high levels of contamination, possibly due to poor hygiene practices.
FOOD RESEARCH INTERNATIONAL
(2021)
Article
Nutrition & Dietetics
Inna Burakova, Yuliya Smirnova, Mariya Gryaznova, Mikhail Syromyatnikov, Pavel Chizhkov, Evgeny Popov, Vasily Popov
Summary: Obesity is causing many concomitant diseases, and new strategies for treatment and prevention are being developed using probiotics to modulate the gut microbiota. A study found an increase in Actinobacteriota and a decrease in Bacteroidota in obese patients taking lactic acid bacteria. Beneficial groups like Bifidobacterium and Lactococcus increased, while harmful groups like Faecalibacterium and Clostridium decreased. Modulating the gut microbiota with lactic acid bacteria may be a way to treat obesity.
Article
Ecology
Zivile Buivydaite, Mille Anna Lilja, Rumakanta Sapkota, Benni Winding Hansen, Lea Ellegaard-Jensen, Niels Bohse Hendriksen, Paul Henning Krogh, Anne Winding
Summary: Earthworms play an important role in soil ecosystem functioning and are used as indicators of ecosystem health. This study investigates the interactions between earthworms and soil microorganisms by comparing soil with and without earthworms in microcosms. The results show that earthworms increase microbial activity, decrease species richness, and shape soil prokaryotes community structure. Certain bacterial phyla were enriched, while the relative abundance of the archaeal phylum Thaumarchaeota decreased in the presence of earthworms. The study highlights the influence of earthworms on soil microbial communities and their activity, suggesting the need to incorporate earthworm-prokaryote interactions in future soil microbiome studies.
EUROPEAN JOURNAL OF SOIL BIOLOGY
(2023)
Article
Immunology
Lorena Ruiz, Claudio Alba, Cristina Garcia-Carral, Esther A. Jimenez, Kimberly A. Lackey, Michelle K. McGuire, Courtney L. Meehan, James Foster, Daniel W. Sellen, Elizabeth W. Kamau-Mbuthia, Egidioh W. Kamundia, Samwel Mbugua, Sophie E. Moore, Andrew M. Prentice, Debela K. Gindola, Gloria E. Otoo, Rossina G. Pareja, Lars Bode, Mark A. McGuire, Janet E. Williams, Juan M. Rodriguez
Summary: This study reprocessed and resequenced a large set of human milk samples from diverse international sites, revealing the impact of different sequencing approaches on the microbiota profiles obtained for human milk samples and showing relatively consistent microbial configurations in the studied cohorts.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Microbiology
Shu Li, Bao Qi, Wan Wang, Xueyan Peng, Andrey A. Gontcharov, Bao Liu, Qi Wang, Yu Li
Summary: The study identified the bacterial communities associated with the plasmodia of six myxomycetes species and found that while the bacterial composition varied among species, there was a high similarity in the functional composition of the bacterial communities. The enrichment for gram-negative and aerobic bacteria in plasmodia suggests that myxomycetes may selectively recruit certain bacteria from the environment.
Article
Marine & Freshwater Biology
Mingming Han, Chenxi Zhu, Shengkai Tang, Ji Liang, Daming Li, Yanxia Guo, Zakaria Zuraini, Qin Si, Qichen Jiang
Summary: This study evaluated the effects of acute exposure to 75-nm polystyrene nanoplastics on the immune responses, antioxidant defenses, and gut microbiota of Eriocheir sinensis. After 48 hours of exposure, nanoplastics were found in the gills, hepatopancreas, and gut, but not in the gut after 7 days of recovery. Exposure to nanoplastics altered the expression of immune-related genes and enzyme activities, as well as the diversity of gut microbiota. The hepatopancreas of E. sinensis could not fully recover within a 7-day recovery period.
AQUATIC TOXICOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Hu Hui, Subash C. B. Gopinath, Zool H. Ismail, Yeng Chen, K. Pandian, Palaniyandi Velusamy
Summary: Myocardial infarction (MI) is a life-threatening condition that can cause cardiac arrest and organ damage. Current diagnostic techniques are not sensitive enough for early detection, but blood biomarker-based diagnosis, specifically the measurement of cardiac troponin I (cTnI), provides a potential solution. In this study, a sensor based on interdigitated electrodes was developed and used to detect cTnI. The use of aptamer-conjugated gold nanoparticles and detection antibodies in a sandwich pattern significantly improved the sensitivity of the detection. The developed sensor showed promising results for diagnosing MI.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Biochemistry & Molecular Biology
Dapeng Qin, Qiuping Gong, Xin Li, Yanping Gao, Subash C. B. Gopinath, Yeng Chen, Zehua Yang
Summary: In this study, a gold nanoparticle-based colorimetric biosensing method was used to accurately and easily detect the specific target DNA for Mycoplasma pneumoniae. The color of the gold nanoparticles changes in the presence of the target DNA, allowing for the diagnosis of Mycoplasma pneumoniae.
BIOTECHNOLOGY AND APPLIED BIOCHEMISTRY
(2023)
Article
Chemistry, Physical
Evan T. Salim, Suhair R. Shafeeq, Mohammed Jalal AbdulRazzaq, Makram A. Fakhri, Subash C. B. Gopinath
Summary: This study presents the first use of silver (Ag) nanoparticles to enhance the pulsed laser fabricated Nb2O5 nanostructures. Ag NPs were incorporated into Nb2O5 nanomatrix structure by UV photo-activation mechanism. The results showed that the optical band gap of Nb2O5 was reduced and defects were reduced within the UV region of the emission wavelengths. XRD analysis confirmed the successful decoration of Ag NPs into the Nb2O5 nanostructure. SERS analysis demonstrated successful hot electrons transfer in the monoclinic (H-Nb2O5).
SURFACES AND INTERFACES
(2023)
Review
Engineering, Biomedical
Hong Hui Jing, Fevzi Bardakci, Sinan Akgol, Kevser Kusat, Mohd Adnan, Mohammad Jahoor Alam, Reena Gupta, Sumaira Sahreen, Yeng Chen, Subash C. B. Gopinath, Sreenivasan Sasidharan
Summary: Carbon dots (CDs) are a type of crystalline, quasi-spherical fluorescence nanomaterials with a size between 1 nm to 10 nm. Green CDs are synthesized from renewable biomass and have distinct advantages over physicochemical methods. Green CDs can be synthesized using various plant-based organic resources and have diverse applications in biomedical fields. This review focuses on the synthesis, characterization techniques, properties, and biomedical applications of green CDs.
JOURNAL OF FUNCTIONAL BIOMATERIALS
(2023)
Review
Engineering, Chemical
Hussaini Adam, Subash C. B. Gopinath, M. K. Md Arshad, Tijjani Adam, Uda Hashim, Zaliman Sauli, Makram A. Fakhri, Sreeramanan Subramaniam, Yeng Chen, Sreenivasan Sasidharan, Yuan Seng Wu
Summary: This study presents an electrochemically-based microfluidic nanobiosensor array for monoplex and multiplex detection of physiologically relevant analytes. The integrated system is portable and serves as a custom electrochemical reader. It has great potential for real-time profiling of immune-mediated treatment responses and as a point-of-care diagnostic device for conducting near-patient tests using biological samples.
JOURNAL OF THE TAIWAN INSTITUTE OF CHEMICAL ENGINEERS
(2023)
Article
Biochemistry & Molecular Biology
Hussaini Adam, Subash C. B. Gopinath, Thirumananseri Kumarevel, M. K. Md Arshad, Tijjani Adam, Zaliman Sauli, Sreeramanan Subramaniam, Uda Hashim, Yeng Chen
Summary: In this study, a novel technique for the selective detection of amyloid fibrils was reported. Using a dipole moment on a dielectrode surface, this technique can sensitively detect the aggregation of alpha-synuclein, which is crucial for diagnosing Parkinson's disease. The stability and detection range of the sensor were confirmed through physical and electrical characterization.
PROCESS BIOCHEMISTRY
(2023)
Review
Green & Sustainable Science & Technology
Wan Yuen Tan, Subash C. B. Gopinath, Periasamy Anbu, Ahmad Radi Wan Yaakub, Sreeramanan Subramaniam, Yeng Chen, Sreenivasan Sasidharan
Summary: With the development of bionanotechnology, the field of nanobiocatalysts has grown rapidly and transformed various nanomaterials into novel nanocarriers for enzyme immobilization. Nanotubes, nanofibers, nanopores, nanoparticles, and nanocomposites have been successfully used as nanocarriers. The combination of enzymes and nanocarriers through different immobilization techniques has attracted attention due to their exceptional catalytic performance, stability, and reusability. The review discusses enzyme immobilization on nanocarriers and highlights the techniques, properties, preparations, and applications of nanoimmobilized enzymes.
Review
Biotechnology & Applied Microbiology
Hussaini Adam, Subash C. B. Gopinath, M. K. Md Arshad, Tijjani Adam, N. A. Parmin, Irzaman Husein, Uda Hashim
Summary: In severe cases, Parkinson's disease causes uncontrolled movements known as motor symptoms such as dystonia, rigidity, bradykinesia, and tremors. Parkinson's disease also causes non-motor symptoms such as insomnia, constipation, depression and hysteria. Disruption of neural networks in the substantia nigra pars compacta is a major cause of motor symptoms in Parkinson's disease, highlighting the need for better methods of detection. Improved diagnosis and treatment of Parkinson's disease can help avoid some of its devastating symptoms.
Review
Microbiology
Suaidah Ramli, Yuan Seng Wu, Kalaivani Batumalaie, Rhanye Mac Guad, Ker Woon Choy, Ashok Kumar, Subash C. B. Gopinath, Md. Moklesur Rahman Sarker, Vetriselvan Subramaniyan, Mahendran Sekar, Neeraj Kumar Fuloria, Shivkanya Fuloria, Suresh V. Chinni, Gobinath Ramachawolran
Summary: This review focuses on the therapeutic efficacy and molecular mechanisms of Withania somnifera (WS) extracts and their phytochemicals against SARS-CoV-2 infection. It also discusses the use of molecular docking in developing potential inhibitors from WS to target the virus and host cell receptors. Additionally, the review explores the use of nanoformulations or nanocarriers in enhancing WS delivery for improved bioavailability and treatment efficacy.
Article
Engineering, Environmental
Mugashini Vasudevan, Sathaniswarman Remesh, Veeradasan Perumal, Pandian Bothi Raja, Mohammad Nasir Mohammad Ibrahim, Subash C. B. Gopinath, Saravanan Karuppanan, Mark Ovinis
Summary: This article presents the development and application of a green lignin-derived graphene biosensor for Troponin I, a biomarker for Acute Myocardial Infarction (AMI). The biosensor showed high analytical performance, high specificity, and a detection limit of 100 attomolar. The biosensor has a sensitivity of 31.45 mu A mM(-1) cm(-2), stability of 87%, with a relative standard deviation for reproducibility of 3.8%.
CHEMICAL ENGINEERING JOURNAL
(2023)
Article
Green & Sustainable Science & Technology
Roslaili Abdul Aziz, Mok Yiwen, Mawaddah Saleh, Mohd Nazry Salleh, Subash C. B. Gopinath, Sunny Goh Eng Giap, Suresh V. Chinni, Ramachawolran Gobinath
Summary: This study evaluated the accumulation of trace elements, such as copper, zinc, and lead, in paddy fields and soil, and analyzed their transfer to rice plants. The findings show that the accumulation of heavy metals in soil varies depending on the location and the order of accumulation differs for different metals. The study also found that soil properties and metal abundance can influence the growth characteristics of rice plants.
Article
Food Science & Technology
Raja Hasnida Raja Hashim, Ahmad Anas Nagoor Gunny, Sam Sung Ting, Subash C. B. Gopinath, Yeong Yin Fong, Sunil Pareek, Muaz Mohd Zaini Makhtar, Hafiza Shukor
Summary: This study investigated the effect of Coleus aromaticus essential oil (CAEO) on an active film made of polylactic acid, chitosan, and cellulose nanocrystal for fruit packaging. The addition of CAEO improved the mechanical properties and antioxidant activity of the film, and exhibited inhibitory effects against pathogenic fungus. The results suggest that the PLA/Cs/CNC/CAEO films can enhance microbial safety and prolong the shelf life of fruits, potentially replacing petroleum-based plastics for fruit packaging applications.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Biotechnology & Applied Microbiology
Nagasathiya Krishnan, Roshini Iyer, Swati Priyadarshini, Arkadeep Kundu, Pradiksha Natarajan, Pinki Kumari Singh, Subash C. B. Gopinath, Pachaiappan Raman
Summary: This study investigated the impact of different abiotic elicitors on CPT production in N. nimmoniana cell suspension culture. The results showed that tryptophan and ethanol treatment significantly increased CPT production. However, the viability of the cells decreased when treated with calcium chloride and cadmium chloride.
BIOCATALYSIS AND AGRICULTURAL BIOTECHNOLOGY
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
