Article
Chemistry, Multidisciplinary
Emmanuel de la O-cuevas, Selene R. R. Islas, Perla Gallegos-Flores, E. L. Esparza-Ibarra, Hugo Tototzintle-Huitle, Jose M. Saniger
Summary: In this study, a new analytical platform for the specific recognition of human interleukin-6 was developed using monoclonal antibodies of interleukin-6 onto amine functionalized graphene substrates. This experimental approach can be used to develop a wide variety of substrates for specific detection of analytes in complex matrices.
Article
Medical Laboratory Technology
Zhicheng Ye, Niuniu Dong, Keqing Liang, Haixia Hu, Jin Xu
Summary: This study evaluated the performance of a new interleukin 6 assay, Pylon IL-6 assay, and confirmed its potential use in near-patient settings to reduce turnaround times. The new assay demonstrated performance comparable to those performed on clinical laboratory instruments.
JOURNAL OF CLINICAL LABORATORY ANALYSIS
(2021)
Article
Chemistry, Analytical
Di Zhang, Shiting Gu, Lingbo Zong, Ze Hu, Gao-Chao Fan, Xiliang Luo
Summary: The enhanced photocathodic immunoassay with an illustrious signaling mechanism of oxygen reduction reaction (ORR) triggered by BOD labeling shows high sensitivity and accuracy in biological matrix, pioneering an effective strategy for advanced photocathodic bioassays for different targets.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Ze Hu, Yaqun Xu, Hao Wang, Gao-Chao Fan, Xiliang Luo
Summary: This study presents a self-powered anti-interference photoelectrochemical immunosensor for accurate and specific detection of cardiac troponin I. By incorporating a novel ternary heterojunction photocathode and a zwitterionic peptide, the immunosensor exhibits high sensitivity for target antigen detection and good anti-interference performance.
CHINESE CHEMICAL LETTERS
(2022)
Article
Chemistry, Analytical
Dongquan Leng, Rui Xu, Lei Liu, Huan Wang, Jingshuai Li, Xiang Ren, Qin Wei, Huangxian Ju
Summary: A novel photoelectrochemical biosensor was optimized to detect CYFRA21-1 by utilizing high-performance BiVO4/Ag3VO4/SnS2 photoelectrodes and a specific enzymatic reaction system, achieving high sensitivity, wide linear range, and low detection limit.
Article
Biochemistry & Molecular Biology
Rossella Salemi, Giuseppe Gattuso, Barbara Tomasello, Alessandro Lavoro, Agostino Gaudio, Massimo Libra, Salvatore Santo Signorelli, Saverio Candido
Summary: This study investigated the involvement of IL-6 trans-signaling in DVT and found a strong correlation between the Asp358Ala variant and sIL6-R levels. The high frequency of the Asp358Ala mutation and elevated IL-6 levels in DVT patients may explain the association between IL-6 overactivation and DVT outcome. These findings provide a proof of concept for targeting IL-6 trans-signaling as a new strategy for DVT adjuvant therapy.
Article
Biochemical Research Methods
Kazuki Takahashi, Shunsuke Chida, Thanawat Suwatthanarak, Mikiko Iida, Min Zhang, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Takao Yasui, Yoshinobu Baba, Akihide Hibara, Mina Okochi, Manabu Tokeshi
Summary: This paper presents the first report on a non-competitive fluorescence polarization immunoassay (NC-FPIA) using a peptide as a tracer. The NC-FPIA enables easy and quick quantification of the target by simple mixing. This method has potential applications in clinical diagnostics, infectious disease screening, on-site food safety analysis, etc. The study successfully applied the NC-FPIA to detect CD9 and CD9 expressing exosomes from HeLa cells, and it holds promise for future application to various targets.
Article
Biochemistry & Molecular Biology
Elizabeta Georgieva, Stefan L. Leber, Cora Wex, Christoph Garbers
Summary: The study reveals that perturbation of the actin cytoskeleton is not sufficient to induce NF-kappa B activation and IL-6 secretion, but it reduces IL-6-induced activation of STAT3 in Hep3B cells. Additionally, IL-6 receptor proteolysis by ADAM10 is not dependent on the integrity of the actin cytoskeleton.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Chemistry, Multidisciplinary
Jan Paul Menzel, Yorrick Boeije, Tijmen M. A. Bakker, Jelena Belic, Joost N. H. Reek, Huub J. M. de Groot, Lucas Visscher, Francesco Buda
Summary: Dye-sensitized photoelectrochemical cells show promise in solar energy conversion, but the loss of charge through recombination at the dye-semiconductor interface remains a challenge. Charge separating dyes constructed as push-pull systems can improve spatial separation of electron and hole, reducing recombination rate. In this study, a family of dyes consisting of polyphenylamine donors, fluorene bridges, and perylene monoimide acceptors was investigated using computational methods. Multiple molecular design strategies were explored to optimize charge separation, resulting in a dye with significantly improved properties compared to the original complex.
Article
Chemistry, Analytical
Fengxi Zhu, Qiang Zhao
Summary: In this study, a CRISPR/Cas12a-powered immunoassay for sensitive small-molecule detection is described. The method utilizes the antibody coated on a microplate to recognize the target and a small molecule-labeled active DNA (acDNA) to activate the CRISPR/Cas12a activity. The proposed method achieves low detection limits and has a flexible detection concentration range. It also shows high selectivity and good applicability in complex media.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yang Zang, Chengli Yu, Jingjing Jiang, Huaiguo Xue
Summary: In this study, a novel photoelectrochemical biosensor was developed for sensitive detection of miR-141. The biosensor showed excellent performance and great potential in clinical application.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Keine Nishiyama, Mao Fukuyama, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani, Akihide Hibara, Manabu Tokeshi
Summary: A non-competitive fluorescence polarization immunoassay (FPIA) using a Fab fragment was developed for large molecule quantification. The method, tested with C-reactive protein (CRP), achieved successful quantitative analysis without the need for sample pretreatment and with high sensitivity. The simplified procedure involved one-step mixing without the need for washing operations, enabling rapid protein quantification.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Review
Immunology
Elena Grebenciucova, Stephen Vanhaerents
Summary: This article discusses the functions and therapeutic applications of interleukin 6 (IL-6), as well as its relationship with various autoimmune diseases. It also explores the potential of IL-6 blockade in the treatment of other autoimmune conditions and emphasizes the importance of clinical trials.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Franciele de Matos Morawski, Greicy Brisa Malaquias Dias, Kelline Alaide Pereira Sousa, Rodrigo Formiga, Fernando Spiller, Alexandre Luis Parize, Andre Bafica, Cristiane Luisa Jost
Summary: A new method for label-free voltammetric determination of interleukin-6 has been developed using a chitosan/genipin modified fluorine tin oxide electrode, providing an in-situ hydrogel formation. The method showed high selectivity and sensitivity, with a wide linear range and low detection limit. It was successfully applied for quantification of interleukin-6 in serum samples of septic mice.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Cell Biology
Dean F. Loporchio, Emily K. Tam, Jane Cho, Jaeyoon Chung, Gyungah R. Jun, Weiming Xia, Marissa G. Fiorello, Nicole H. Siegel, Steven Ness, Thor D. Stein, Manju L. Subramanian
Summary: This study compared the vitreous cytokine profiles of patients with proliferative diabetic retinopathy (PDR) to those without PDR. Novel increases in IL-15 and IL-16, along with expected VEGF, were observed in the vitreous humor of patients with PDR. Additionally, elevations of ICAM-1, VCAM-1, SAA, IL-8, and CRP were confirmed in the vitreous of PDR patients, as previously described.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)