Article
Agronomy
Matteo Salina, Giovanni Tagliabue, Roberta Ghizzoni, Valeria Terzi, Caterina Morcia
Summary: Mycotoxigenic fungi, especially Fusaria, can contaminate wheat grains with various mycotoxins, impacting human and animal health. A fast multi-toxin assay based on RPI technology has been developed to identify and quantify toxins in wheat samples, showing potential for point-of-care and point-of-sale control along production and transformation chains.
Article
Chemistry, Analytical
Jun Kurosu, Kaname Kanai, Jun'ya Tsutsumi
Summary: We developed a label-free and highly surface-sensitive bioimaging technique, named as electric-double-layer modulation imaging (EDLMI). This technique utilized the charge density of electric double layer, accumulated at the interface between a semiconductor and electrolyte solution, to sensitively respond to bio-molecular adsorption. By utilizing lock-in imaging in synchronization with electric-double-layer modulation, EDLMI can monitor nano-thick biomolecular adsorption in real time with a wide-range magnification, distinguishing specific and non-specific binding between antibodies.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Chemistry, Applied
Amadeo Sena-Torralba, Nathalie G. E. Smits, David Blazquez, Clara Albero-Perez, Yeray Pallas-Tamarit, Angel Maquieira, Sergi Morais
Summary: This study presents a cost-effective and straightforward optoelectrical microanalytical system for the simultaneous quantification of the six most common food allergens. The system demonstrates high accuracy and reliability in identifying allergenic ingredients in commercial foods. The method offers a potential high throughput biosensing tool for screening multiple allergens.
Article
Chemistry, Physical
Vinit Kumar Chugh, Kai Wu, Venkatramana D. Krishna, Arturo di Girolamo, Robert P. Bloom, Yongqiang Andrew Wang, Renata Saha, Shuang Liang, Maxim C-J Cheeran, Jian-Ping Wang
Summary: Magnetic particle spectroscopy (MPS) is a highly sensitive and versatile sensing technique for quantitative bioassays, utilizing the dynamic magnetic responses of magnetic nanoparticles (MNPs) to detect target analytes. An MPS system with a one-stage lock-in design enhances sensitivity and can be applied for detecting various analytes such as viruses and toxins.
JOURNAL OF PHYSICAL CHEMISTRY C
(2021)
Article
Chemistry, Multidisciplinary
David S. Kinnamon, Jacob T. Heggestad, Jason Liu, Thu Nguyen, Vo Ly, Angus M. Hucknall, Cassio M. Fontes, Rhett J. Britton, Jian-Piao Cai, Jasper Fuk-Woo Chan, Kwok-Yung Yuen, Thuy Le, Ashutosh Chilkoti
Summary: We developed a self-contained immunoassay platform that can be used in field settings with varied temperature, humidity, and blood samples. The platform has high sensitivity and accuracy in detecting analyte concentration, and a portable detection device. We also improved the microfluidic cassette for better usability and faster results. By using this platform, we successfully conducted a rapid diagnostic test for talaromycosis infection in patients, showing comparable sensitivity and specificity to laboratory tests.
Article
Chemistry, Analytical
Nan Li, Minjie Shen, Youchun Xu
Summary: A portable microfluidic system was established for point-of-care detection of multiple protein biomarkers, combining a protein microarray and a microfluidic cassette. The system automatically conducted immunoassays for alpha-fetoprotein, carcinoembryonic antigen, and carcinoma antigen 125 within 40 minutes, with good correlations to results from a commercial instrument. This versatile platform shows great potential for rapid and sensitive multiplexed detection of biomarkers in POC diagnostics.
Review
Chemistry, Analytical
Maria Jesus Pioz, Rocio L. Espinosa, Maria Fe Laguna, Beatriz Santamaria, Ana Maria M. Murillo, Alvaro Lavin Hueros, Sergio Quintero, Luca Tramarin, Luis G. Valle, Pedro Herreros, Alberto Bellido, Rafael Casquel, Miguel Holgado
Summary: This article explores the development and market transferability of Point-of-Care devices based on optical technology. It proposes an indicator to assess the extent of research findings being protected by patents. By searching articles and patents, classifying them, and analyzing the relationship between them, the study provides insights into the state-of-the-art technology and its transfer to the market in the optical Point-of-Care device sector.
Review
Chemistry, Multidisciplinary
Zdenek Farka, Julian C. Brandmeier, Matthias J. Mickert, Matej Pastucha, Karel Lacina, Petr Skladal, Tero Soukka, Hans H. Gorris
Summary: This review discusses the potential and application of nanoparticles in replacing current labels in bioaffinity assays. Nanoparticles can be used as capture support, signal generation, and signal amplification elements, and have been successfully applied in SARS-CoV-2 rapid antigen tests. However, the limited application of nanoparticles in current commercial assays is attributed to the challenges associated with analyzing real samples.
ADVANCED MATERIALS
(2023)
Review
Biotechnology & Applied Microbiology
Sha Liu, Kaixuan Zhao, Meiyuan Huang, Meimei Zeng, Yan Deng, Song Li, Hui Chen, Wen Li, Zhu Chen
Summary: The global burden of foodborne disease is significant, with foodborne pathogens being the primary cause. The detection of foodborne pathogenic bacteria has rapidly developed in recent years, with methods such as immunoassay, molecular biology, microfluidic chip, metabolism, biosensor, and mass spectrometry being the main techniques. This study provides a comprehensive review of various rapid detection methods and discusses their limitations, advantages, and future development direction for accurate and rapid diagnosis and control of diseases in point-of-care testing (POCT) applications.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Review
Chemistry, Multidisciplinary
Neeti Kalyani, Surbhi Goel, Swati Jaiswal
Summary: Innovative technologies in pesticide detection have led to improved sensitivity, reduced detection time, and the ability to simultaneously detect multiple pesticides. Optical biosensors are at the forefront of technology, offering advantages such as easy operation, high sensitivity, and cost-effectiveness for rapid pesticide detection.
ENVIRONMENTAL CHEMISTRY LETTERS
(2021)
Article
Biophysics
Augusto Juste-Dolz, Martina Delgado-Pinar, Miquel Avella-Oliver, Estrella Fernandez, Daniel Pastor, Miguel V. Andres, Angel Maquieira
Summary: The Bio Bragg Gratings (BBGs) present a novel biosensing approach using diffractive structures of protein bioreceptors to transduce the magnitude of biorecognition assays into the intensity of single peaks in the reflection spectrum. Through theoretical and experimental studies, the design, fabrication, and optimization of this system are explored to provide insights into its bioanalytical prospects. The system shows potential for avoiding unwanted signal contributions from non-specific binding and for conceiving multiplexed BBG systems capable of performing multiple label-free biorecognition assays in a single device.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Luis A. Tortajada-Genaro, Natalia Casan-Raga, Salva Mas, Ethel Ibanez-Echevarria, Sergi Morais, Angel Maquieira
Summary: This study presents an in vitro biosensing method for specific IgE levels based on a reversed-phase allergen array. It combines optical biosensing, direct multiplex immunoassays, and on-disc technology to identify 12 sIgE associated with food allergies in a single analysis with a low serum sample volume. The method has excellent clinical specificity (100%) and high sensitivity (91.1%), making it potentially useful for diagnosing multiple food allergies.
Review
Chemistry, Analytical
Yein Kwon, Yejin Yoon, Moonbong Jang, Sunggu Kang, Chulhwan Park, Taek Lee
Summary: Cylindrospermopsin (CYN) is a freshwater algal toxin that is produced during the proliferation of harmful cyanobacterial algal blooms. The effects of global warming have contributed to the worldwide occurrence of cyano-HABs, leading to increased damage to aquatic ecosystems. CYN exhibits cell toxicity, as well as toxicity to the skin, genes, liver, and other organs. Current methods for CYN detection have limitations, and recent studies have focused on biosensor-based technologies for improved detection.
Article
Materials Science, Multidisciplinary
Guojun Ke, Dingkai Su, Yu Li, Yu Zhao, Honggang Wang, Wanjian Liu, Man Li, Zhiting Yang, Fang Xiao, Yao Yuan, Fei Huang, Fanyang Mo, Peng Wang, Xuefeng Guo
Summary: Researchers have developed a portable COVID-19 detector based on graphene technology, which can accurately and quickly detect SARS-CoV-2 with extremely low detection limits. Validation results show that the detection method is consistent with traditional PCR methods and has a rapid detection speed.
SCIENCE CHINA-MATERIALS
(2021)
Article
Engineering, Biomedical
Congran Jin, Ziqian Wu, John H. Molinski, Junhu Zhou, Yundong Ren, John X. J. Zhang
Summary: Advancement in material science, particularly in the development of nanomaterials, has led to the creation of plasmonic biosensors that enable real-time diagnosis of various diseases through biomarker screening. These versatile and portable devices can be used in point-of-care settings, allowing for convenient and efficient testing of biomarkers in homes or elsewhere. This article reviews the recent progress in the design of these biosensors, including the development of nanomaterials and the applications in point-of-care diagnosis. Additionally, challenges and opportunities, such as incorporating machine learning algorithms and the testing of Covid-19, are discussed.
MATERIALS TODAY BIO
(2022)
Correction
Chemistry, Physical
Emanuela Frittoli, Andrea Palamidessi, Fabio Iannelli, Federica Zanardi, Stefano Villa, Leonardo Barzaghi, Hind Abdo, Valeria Cancila, Galina V. Beznoussenko, Giulia Della Chiara, Massimiliano Pagani, Chiara Malinverno, Dipanjan Bhattacharya, Federica Pisati, Weimiao Yu, Viviana Galimberti, Giuseppina Bonizzi, Emanuele Martini, Alexander A. Mironov, Ubaldo Gioia, Flora Ascione, Qingsen Li, Kristina Havas, Serena Magni, Zeno Lavagnino, Fabrizio Andrea Pennacchio, Paolo Maiuri, Silvia Caponi, Maurizio Mattarelli, Sabata Martino, Fabrizio d'Adda di Fagagna, Chiara Rossi, Marco Lucioni, Richard Tancredi, Paolo Pedrazzoli, Andrea Vecchione, Cristiano Petrini, Francesco Ferrari, Chiara Lanzuolo, Giovanni Bertalot, Guilherme Nader, Marco Foiani, Matthieu Piel, Roberto Cerbino, Fabio Giavazzi, Claudio Tripodo, Giorgio Scita
Article
Chemistry, Physical
Emanuela Frittoli, Andrea Palamidessi, Fabio Iannelli, Federica Zanardi, Stefano Villa, Leonardo Barzaghi, Hind Abdo, Valeria Cancila, Galina V. Beznoussenko, Giulia Della Chiara, Massimiliano Pagani, Chiara Malinverno, Dipanjan Bhattacharya, Federica Pisati, Weimiao Yu, Viviana Galimberti, Giuseppina Bonizzi, Emanuele Martini, Alexander A. Mironov, Ubaldo Gioia, Floriana Ascione, Qingsen Li, Kristina Havas, Serena Magni, Zeno Lavagnino, Fabrizio Andrea Pennacchio, Paolo Maiuri, Silvia Caponi, Maurizio Mattarelli, Sabata Martino, Fabrizio d'Adda di Fagagna, Chiara Rossi, Marco Lucioni, Richard Tancredi, Paolo Pedrazzoli, Andrea Vecchione, Cristiano Petrini, Francesco Ferrari, Chiara Lanzuolo, Giovanni Bertalot, Guilherme Nader, Marco Foiani, Matthieu Piel, Roberto Cerbino, Fabio Giavazzi, Claudio Tripodo, Giorgio Scita
Summary: The transition from locally confined epithelial malignancies to invasive cancers, known as unjamming, is accompanied by mechanical deformations of cells and nuclei due to large fluctuations in cell density. This triggers a cellular mechano-protective mechanism involving changes in nuclear size and rigidity, heterochromatin redistribution, and remodeling of actin architecture. The chronic strains and stresses associated with unjamming eventually lead to DNA damage and nuclear envelope ruptures, activating a cytosolic DNA response gene program. This transcriptional rewiring alters the cell state, promoting malignant traits in breast carcinoma.
Article
Chemistry, Analytical
Laura Sola, Laura Abdel Mallak, Francesco Damin, Alessandro Mussida, Dario Brambilla, Marcella Chiari
Summary: We conducted a detailed investigation on the effect of a polymeric coating's functional group concentration on probe density immobilization, aiming to find the optimal formulation for specific microarray applications. The performance of a microarray is known to be influenced by the way probes are attached to the surface, and our N, N-dimethylacrylamide-based polymeric coating has shown great flexibility for customizing surface properties. Through synthesizing polymers with increasing concentrations of N-acryloyloxysuccinimide (NAS) and optimizing the coating procedure accordingly, we tested their performance in DNA and protein microarray assays to determine the formulation that improves assay performance in specific applications. The flexibility of this polymeric platform also allowed us to explore different immobilization chemistries, specifically click chemistry reactions, and assess the potential differences generated by this modification.
Article
Oncology
Nina Cortese, Roberta Carriero, Marialuisa Barbagallo, Anna Rita Putignano, Guido Costa, Fabio Giavazzi, Fabio Grizzi, Fabio Pasqualini, Clelia Peano, Gianluca Basso, Sergio Marchini, Federico Simone Colombo, Cristiana Soldani, Barbara Franceschini, Luca Di Tommaso, Luigi Terracciano, Matteo Donadon, Guido Torzilli, Paolo Kunderfranco, Alberto Mantovani, Federica Marchesi
Summary: Two M4 markers associated with opposite clinical relevance were identified through single-cell analysis, indicating the importance of improved classification and prognostic classifiers for patients with colorectal liver metastasis.
CANCER IMMUNOLOGY RESEARCH
(2023)
Review
Multidisciplinary Sciences
A. Vailati, H. Bataller, M. M. Bou-Ali, M. Carpineti, R. Cerbino, F. Croccolo, S. U. Egelhaaf, F. Giavazzi, C. Giraudet, G. Guevara-Carrion, D. Horvath, W. Koehler, A. Mialdun, J. Porter, K. Schwarzenberger, V. Shevtsova, A. De Wit
Summary: Understanding transport and mixing in fluids, both in the presence and absence of external fields and reactions, is a challenging and strategically important topic for space exploration. The efficient mixing and transport of components in microgravity conditions is crucial for sustaining long-term space missions. Investigating these processes at the mesoscopic scale is increasingly important for understanding mass transfer in confined systems, such as porous media and microfluidic systems, where the convective flows induced by buoyancy on Earth are absent. This research has practical applicative relevance for handling complex fluids and processing materials in space.
Article
Chemistry, Multidisciplinary
Stefano Marni, Giovanni Nava, Raouf Barboza, Tommaso Giovanni Bellini, Liana Lucchetti
Summary: The motion of ferroelectric liquid sessile droplets deposited on a ferroelectric lithium niobate substrate can be controlled by a light beam of moderate intensity. The droplets are attracted or repelled depending on the side of the substrate exposed to light irradiation. Furthermore, moving the beam allows for long-distance movement of the droplets on the substrate, indicating the coupling between the droplet polarization and the polarization induced in the substrate.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Giovanni Nava, Luca Casiraghi, Thomas Carzaniga, Giuliano Zanchetta, Marcella Chiari, Francesco Damin, Valentina Bollati, Lucia Signorini, Serena Delbue, Tommaso Bellini, Marco Buscaglia
Summary: This article describes an optical device that enables the enumeration of single virus particles binding on a surface coated with antibody or aptamers. By utilizing the localized contributions of nanoparticles adhering to the sensor surface, a scan-free, label-free microarray-type digital assay is realized. The combination of different probes on the same surface increases the specificity of detection and dynamic range.
Editorial Material
Physics, Multidisciplinary
Irmgard Bischofberger, Ramon Castaneda-Priego, Roberto Cerbino, Luca Cipelletti, Emanuela Del Gado, Alberto Fernandez-Nieves
FRONTIERS IN PHYSICS
(2023)
Article
Chemistry, Analytical
Francesco Damin, Silvia Galbiati, Nicola Clementi, Roberto Ferrarese, Nicasio Mancini, Laura Sola, Marcella Chiari
Summary: Since the emergence of the COVID-19 pandemic, monitoring and tracking the distribution of SARS-CoV-2 variants is crucial for adjusting public health interventions. However, genome sequencing is not cost-effective and rapid. We have developed a microarray-based assay that can accurately detect known viral variants present in clinical samples by detecting mutations in the Spike protein gene. This assay can genotype the WHO-reported variants of concern (VOCs) in patient samples.
Article
Polymer Science
Chiara Guidolin, Christopher Heim, Nathan B. P. Adams, Philipp Baaske, Valeria Rondelli, Roberto Cerbino, Fabio Giavazzi
Summary: Introduced more than 50 years ago, dynamic light scattering (DLS) is commonly used for determining the size distribution of colloidal suspensions and macromolecules in solution. This study demonstrates that differential dynamic microscopy (DDM), a microscope-based DLS technique, can accurately measure the size of small macromolecules in diluted solutions. The results show that DDM is a valuable alternative for rapid, label-free protein sizing with an optical microscope.
Article
Physics, Multidisciplinary
Federico Caimi, Giovanni Nava, Susanna Fuschetto, Liana Lucchetti, Petra Paie, Roberto Osellame, Xi Chen, Noel A. Clark, Matthew A. Glaser, Tommaso Bellini
Summary: The recently discovered ferroelectric nematic (N-F) liquid-crystal phase possesses both the fluid-like orientation and the large magnitude polarization field. This unique combination results in a distinct electrostatic phenomenon and response to applied fields. In this study, we observe that the polarization field of the N-F phase aligns with the microchannels despite their winding paths when a small electric field is applied. This alignment is due to the fluid superscreening effect, where the electric field components normal to the channel walls are promptly eliminated by polarization reorientation. This finding sheds light on the internal structure and electrical self-organization of channel-confined N-F liquid crystals.
Article
Multidisciplinary Sciences
Francesco Righini, Marina Carpineti, Fabio Giavazzi, Alberto Vailati
Summary: This article investigates the kinematics of pronking, a leaping behavior observed in some quadrupeds. The study shows that pronking is an effective escape strategy in landscapes with a certain density of obstacles, maximizing the probability of trespassing in the shortest time. The transition between pronking and other gaits is continuous when obstacle height distribution is non-increasing, and discrete when the distribution is peaked. The findings have implications for autonomous robotic exploration on unstructured terrain.
ROYAL SOCIETY OPEN SCIENCE
(2023)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)