期刊
BIOSENSORS & BIOELECTRONICS
卷 59, 期 -, 页码 21-27出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2014.02.071
关键词
Tumor-associated carbohydrate antigens; Tn antigen; Photoelectrochemical biosensor; Graphene
类别
资金
- Open Research Fund Program of Key Laboratory of Marine Drugs (Ocean University of China)
- Ministry of Education of People's Republic of China [KLMD(OUC)201206]
- National Natural Science Foundation of China [21275003, 21025523, 21075073]
- Excellent Young Scientists Encouragement Foundation of Shandong Province [BS2011CL015]
- Program for Changjiang Scholars and Innovative Research Team in University (PCSIRT)
Tumor-associated carbohydrate antigens (TACAs) are often found on the surface of cancer cells. The determination of the carbohydrate components of glycoconjugates is challenging because of the chemical complexity of glycan chains. Through monitoring corresponding antibody, we can get a good solution for clinical diagnosis. Here breast tumor-associated carbohydrate antigens Tn were used as a model and a new photoelectrochemical biosensor for ultrasensitive detection of antibody against Tn was developed. To enhance the sensitivity, both graphene oxide and graphene were used during the construction of biosensor. Through the formation of immunocomplex and the insoluble biocatalytic precipitation (BCP) product, photocurrent intensity was decreased greatly and the antibody could be detected from 0.5 to 500 pg/mL with a detection limit of 1.0 x 10(-13) g/mL At the same time, the developed biosensor showed acceptable selectivity and could be used in the complex matrix. Compared with the traditional glycoarray method, this PEC method is more sensitive (5 orders of magnitude), and thus provides another platform to monitor the immune response to carbohydrate epitopes at different stages during differentiation, metastasis, or treatment. (C) 2014 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据