期刊
BIOSENSORS & BIOELECTRONICS
卷 53, 期 -, 页码 71-75出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.09.036
关键词
Fluorescence sensor; Dual-emission; Ag nanoclusters; Cysteine
类别
资金
- Scientific Research Foundation of Education Commission of Hubei Province [Q20111010, T201101]
- Natural Science Foundation of Hubei Province [2011CDB059, 2011CDA111]
- Research Fund for the Doctoral Program of Higher Education of China [20114208120006]
- National Undergraduate Training Programs for Innovation and Entrepreneurship [201210512006, 201210512020]
An effective dual-emission fluorescent Ag nanoclusters (NCs)-based probe have been constructed for rapid and selective detection of cysteine (Cys) with tunable sensitivity. Electrostatically induced reversible phase transfer method is employed to synthesize Ag nanoclusters with tunable emission intensity at 430 nm and 630 nm by controlling molar ratio between Ag and glutathione. The fluorescence of the Ag nanoclusters could be selectively quenched in the presence of Cys with a detection limit as low as 10 nM. Good linear correlations are obtained over the concentration range from 0.5 to 55 mu M (quenched emission at 630 nm), 55 to 120 mu M and 120 to 220 mu M (enhanced emission at 555 nm) and 120 to 200 mu M (quenched emission at 430 nm), respectively. The long-wavelength emission of the Ag nanoclusters can avoid the interference of the autofluorescence of the biosystems, which facilitated their applications in monitoring Cys in urine. (C) 2013 Elsevier B.V. All rights reserved.
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