期刊
BIOSENSORS & BIOELECTRONICS
卷 45, 期 -, 页码 141-147出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.01.061
关键词
Gap-LCR; DNAzyme; Single-nucleotide polymorphism; Exonuclease; GJB2 235delC
类别
资金
- Chinese Academy of Science [KSCX2-EW-J-22]
- National Sciences Foundation of China [21172215]
Fast and accurate detection of single-nucleotide polymorphism (SNP) is thought more and more important for understanding of human physiology and elucidating the molecular based diseases. A great deal of effort has been devoted to developing accurate, rapid, and cost-effective technologies for SNP analysis. However most of those methods developed to date incorporate complicated probe labeling and depend on advanced equipment. The DNAzyme based Gap-LCR detection method averts any chemical modification on probes and circumvents those problems by incorporating a short functional DNA sequence into one of LCR primers. Two kinds of exonudease are utilized in our strategy to digest all the unreacted probes and release the DNAzymes embedded in the LCR product. The DNAzyme applied in our method is a versatile tool to report the result of SNP detection in colorimetric or fluorometric ways for different detection purposes. (C) 2013 Elsevier B.V. All rights reserved.
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