期刊
BIOSENSORS & BIOELECTRONICS
卷 50, 期 -, 页码 194-201出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.06.020
关键词
Diabetes mellitus; glucose nanobiosensor; Engineered skeletal muscle tissues; glucose uptake; electrical stimulation
类别
资金
- World Premier International Research Center Initiative (WPI), MEXT, Japan
- Grants-in-Aid for Scientific Research [25248032] Funding Source: KAKEN
Skeletal muscle tissues play a significant role to maintain the glucose level of whole body and any dysfunction of this tissue leads to the diabetes disease. A culture medium was created in which the muscle cells could survive for a long time and meanwhile it did not interfere with the glucose sensing. We fabricated a model of skeletal muscle tissues in vitro to monitor its glucose uptake. A nanoporous gold as a high sensitive nanobiosensor was then successfully developed and employed to detect the glucose uptake of the tissue models in this medium upon applying the electrical stimulation in a rapid, and non-invasive approach. The response of the glucose sensor was linear in a wide concentration range of 1-50 mM, with a detection limit of 3 mu M at a signal-to-noise ratio of 3.0. The skeletal muscle tissue was electrically stimulated during 24 h and glucose uptake was monitored during this period. During the first 3 h of stimulation, electrically stimulated muscle tissue consumed almost twice the amount of glucose than counterpart non-stimulated sample. In total, the glucose consumption of muscle tissues was higher for the electrically stimulated tissues compared to those without applying the electrical field. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据