期刊
BIOSENSORS & BIOELECTRONICS
卷 47, 期 -, 页码 520-523出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2013.03.062
关键词
Biosensor; Fluorescence; Lead; DNAzyme; DNA recycling
类别
资金
- Chinese Academy of Sciences
- Promotional Foundation for the Excellent Middle-Aged and Young Scientists of Shandong Province of China [BS2009SW040]
- National Natural Science Foundation [21104030]
- Natural Science Foundation of Shandong Province [ZR2011BQ01]
- Project of Shandong Province Higher Educational Science and Technology Program [J11LB03, J12LA01]
Here, we have demonstrated an ultra-high sensitive detection platform with the detection limit of 5 pM for an environmental toxin-Pb2+. We designed a Pb2+ triggered exonuclease aided DNA recycling system to improve the detection sensitivity. In our system, a Pb2+ dependent 8-17 DNAzyme and its substrate were used to form hybridization duplex. In the presence of Pb2+, the substrate was cleaved and disassociated from the duplex. Then, the released 8-17 DNAzyme was used as a target of the exonuclease aided DNA recycling system which can amplify the fluorescence signal by recycling the 8-17 DNAzyme continuously. Then, the sensitive Pb2+ detection are accomplished and the detection limit of Pb2+ was down to 5 pM which is about 1000 times lower than the traditional detection method based on the 8-17 DNAzyme. (C) 2013 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据