Article
Chemistry, Analytical
Rong Li, Xiaoxia Li, Liu Su, Hetong Qi, Xuanfeng Yue, Honglan Qi
Summary: An electrochemical aptasensor was developed for the determination of serotonin through specific binding and electrochemical measurement. The sensor, using a 57-mer aptamer, showed good selectivity and simplicity in detecting serotonin in the range of 1 to 100 mu M with a detection limit of 0.3 mu M. This work demonstrates the potential of combining aptamer recognition and serotonin oxidation for accurate and efficient detection.
Article
Chemistry, Applied
Yuanyuan Hui, Ding Yang, Weizhe Wang, Yingying Liu, Chao He, Bini Wang
Summary: To detect the residues of streptomycin in dairy products, a new and accurate electrochemical aptasensor was developed. By rationally truncating a 79-mer streptomycin aptamer, a smaller size, lower synthesis cost, and higher affinity truncated aptamer (S03 aptamer) was obtained. The S03 aptamer was used as the recognition molecule in an electrochemical aptasensor, with polyaniline@Ndoping carbon nanotube/Au nanoparticles as the support matrix, for the detection of streptomycin.
JOURNAL OF FOOD COMPOSITION AND ANALYSIS
(2023)
Article
Chemistry, Applied
Seyedeh Fatemeh Ahmadi, Mohammad Hojjatoleslamy, Hossein Kiani, Hooman Molavi
Summary: In this study, a label-free electrochemical aptasensor was developed for accurate and rapid detection of Aflatoxin M1. By optimizing aptamer immobilization time, concentration, and binding time, the sensor showed a linear range and limit of detection. Compared to traditional HPLC methods, this sensor offers a faster and more accurate way to detect Aflatoxin M1.
Article
Biophysics
Zhu Chang, Bicheng Zhu, JinJin Liu, Xu Zhu, Maotian Xu, Jadranka Travas-Sejdic
Summary: A novel electrochemical aptasensor for detecting 17β-estradiol (E2) was developed, showing high sensitivity and selectivity. The sensor was successfully translated from glass carbon electrodes (GCE) to low-cost, disposable laser-scribed graphene electrodes (LSGE), indicating its potential for commercial and portable detection systems. The study demonstrates the feasibility of the sensing platform for E2 and similar biological targets.
BIOSENSORS & BIOELECTRONICS
(2021)
Review
Chemistry, Physical
Jerome Dejeu, Angeline Van der Heyden, Nicolas Spinelli, Eric Defrancq, Liliane Coche-Guerente
Summary: Aptamer-based electrochemical sensors have been developed for detecting a wide variety of analytes with high selectivity, sensitivity, and stability, using specific G-quadruplex (G4) conformations for molecular recognition. These sensors can be miniaturized, have low production costs, and offer great flexibility in design assemblies.
CURRENT OPINION IN ELECTROCHEMISTRY
(2021)
Review
Chemistry, Analytical
Qian Liu, Xinmin Yue, Yixian Li, Fang Wu, Meng Meng, Yongmei Yin, Rimo Xi
Summary: The study introduces a novel electrochemical aptasensor based on specific host-guest interactions between cucurbit [7]uril and ferrocene, providing high selectivity and sensitivity in detecting exosomes. The platform allows for specific capture and release of exosomes, showing good performance in exosome detection while maintaining their integrity. This approach can potentially be applied to other target biomolecules by altering the recognition pairs.
Review
Chemistry, Medicinal
Marjan Majdinasab, Jean Louis Marty
Summary: The early diagnosis of diseases is crucial for effective treatment, and biomarkers offer great potential in this area. Aptamer-based biosensors, with their high sensitivity and selectivity, have emerged as a promising diagnostic tool. Electrochemical aptasensors have attracted significant attention due to their high sensitivity and accuracy in biomarker detection.
Article
Biochemistry & Molecular Biology
Guiyin Li, Huafu Feng, Xiaohang Shi, Min Chen, Jintao Liang, Zhide Zhou
Summary: An electrochemical aptasensor was developed for GPC3 detection, showing good specificity, sensitivity, stability, and reproducibility. It can be applied to various aspects of HCC management, such as therapeutic evaluation and prognosis.
BIOELECTROCHEMISTRY
(2021)
Article
Chemistry, Analytical
Poonam Singh, Sandeep Kumar, Sanjay Kumar Verma
Summary: A fluorescent graphene oxide based aptasensing platform was developed for sensitive and accurate detection of acephate. The screened aptamer AAPT3 demonstrated high affinity and selectivity, and the aptasensor showed low detection limit and wide linear range.
Article
Nanoscience & Nanotechnology
Guiyin Li, HaiMei Li, Wei Chen, Huijiang Chen, Guanxiong Wu, Mingxiong Tan, Jintao Liang, Zhide Zhou
Summary: By combining different nanoparticles, an electrochemical aptasensor was developed for quantitatively detecting GPC3. The sensor showed a linear response range and a low detection limit, making it promising for clinical diagnosis of hepatocellular carcinoma.
JOURNAL OF BIOMEDICAL NANOTECHNOLOGY
(2021)
Review
Chemistry, Analytical
Mohd Syafiq Awang, Yazmin Bustami, Hairul Hisham Hamzah, Nor Syafirah Zambry, Mohamad Ahmad Najib, Muhammad Fazli Khalid, Ismail Aziah, Asrulnizam Abd Manaf
Summary: Large-scale food-borne outbreaks caused by Salmonella are rare nowadays thanks to advanced medical systems, but localized outbreaks still pose a threat to public health. Various detection methods, such as immunological, molecular, mass spectrometry, spectroscopy, optical phenotyping, and biosensors, are available for Salmonella detection. Electrochemical biosensors, especially those utilizing aptamers and nanomaterials, are gaining interest due to sensitivity, speed, and portability.
Article
Chemistry, Analytical
Mohd Afaque Ansari, Wei Juen Liew, Chitra Padmakumari Kurup, Minhaz Uddin Ahmed
Summary: In this study, a nanocomposite consisting of carboxyl-functionalized graphene nanoplatelets (GNPs), carbon nano-onions (CNOs), and chitosan (CS) was synthesized for the development of an electrochemical aptasensor for selective detection of thrombin. The nanocomposite modified glassy carbon electrode (GCE) exhibited remarkable electrochemical response and increased surface area. The designed aptasensor demonstrated sensitive detection of thrombin with excellent sensitivity and specificity.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jina Ding, Dongwei Zhang, Yang Liu, Xuejia Zhan, Yitong Lu, Pei Zhou, Dan Zhang
Summary: A new double-shelled carbon nanocages material was synthesized and developed into an aptasensor for determining Pb2+ in aqueous solution. The aptasensor exhibited a good linear relationship with Pb2+ concentration and detection limits, showing promising applications in environmental monitoring.
Review
Chemistry, Analytical
Kamila Malecka, Edyta Mikula, Elena E. Ferapontova
Summary: The improvement in outcomes for various types of cancer in recent years is attributed to early tumor detection and widespread use of screening tests, with non-invasive, fast, and accurate diagnostic devices playing a crucial role. Biosensors utilizing aptamers in cancer diagnosis offer advantages such as in-vitro aptamer production, cost-effective chemical synthesis, and thermal stability, while electrochemical biosensing approaches provide sensitive, accurate, and affordable sensing methods. This review evaluates the design strategies and analytical performance of electrochemical aptamer-based biosensors for cancer diagnosis, highlighting their future prospects in cancer diagnostics.
Article
Biochemistry & Molecular Biology
Dmitry Gribanyov, Gleb Zhdanov, Andrei Olenin, Georgii Lisichkin, Alexandra Gambaryan, Vladimir Kukushkin, Elena Zavyalova
Summary: The development of sensitive techniques, such as Surface-enhanced Raman spectroscopy (SERS) and DNA aptamers, shows promise for combining rapid specific detection and quantitative determination of viruses, addressing the limitations of existing SERS-based aptasensors.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Polymer Science
Yu Xue, Weidong Li, Guihua Yang, Zhaoyun Lin, Letian Qi, Peihua Zhu, Jinghua Yu, Jiachuan Chen
Summary: The physical strength of regenerated cellulose fibers can be improved by adding hydrogen bond donors and acceptors, resulting in a more compact and stronger structure. However, the effect on crystallinity is limited.
Article
Nanoscience & Nanotechnology
Qian Wang, Zuhao Zhang, Lu Zhang, Yunqing Liu, Li Xie, Shenguang Ge, Jinghua Yu
Summary: In this work, a CRISPR/Cas12a initiated switchable ternary electrochemiluminescence (ECL) biosensor combined with a Co3O4@Au nanoemitter is presented for the in vitro monitoring of miRNA-141. The biosensor demonstrated extraordinary sensitivity and specificity, with a low detection limit.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Chemistry, Analytical
Chuan Huang, Xuefeng Wang, Zuhao Zhang, Lu Zhang, Dejin Zang, Shenguang Ge, Jinghua Yu
Summary: In this study, a novel anode-cathode cooperative paper photoelectrochemical platform was fabricated for ultrasensitive monitoring of miRNA155 using different conductive semiconductors. The platform exhibited excellent sensitivity and selectivity based on the multifold amplification mechanisms and cathode detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Lin Li, Hongmei Yang, Li Li, Xiaoran Tan, Shenguang Ge, Lina Zhang, Jinghua Yu, Yan Zhang
Summary: This study develops a versatile collapsible lab-on-paper biosensor with photothermal effects for early-stage diagnosis and real-time surveillance of epidemic diseases. By implanting a zone with photothermal effects in the miniature space, it enables the on-site detection of microRNA-141 in body fluids and interconnects the traditional photocurrent signal with functional temperature response. The use of Fe3O4 nanoparticles and Prussian blue nanoparticles enhances the visualized thermoresponsive results. Control release of Fe3O4 nanoparticles and modulation of photoelectric signal attenuation improve the accuracy of target temperature signal prediction and enable ultrasensitive detection results.
Article
Chemistry, Analytical
Fengyi Wang, Yaqi Liu, Lu Zhang, Zuhao Zhang, Chuan Huang, Dejin Zang, Haiqing Wang, Shenguang Ge, Jinghua Yu
Summary: A high-sensitivity photoelectrochemical biosensor based on CdS quantum dots sensitized porous hexagonal boron nitride nanosheets and a multiple sites tripodal DNA walker was developed. The biosensor showed excellent linear relationship and detection limit, providing a powerful strategy for early clinical diagnosis and biomedical research.
ANALYTICA CHIMICA ACTA
(2022)
Article
Nanoscience & Nanotechnology
Yaqi Liu, Fengyi Wang, Shuo Ge, Lu Zhang, Zuhao Zhang, Yunqing Liu, Yan Zhang, Shenguang Ge, Jinghua Yu
Summary: A strand displacement amplification (SDA)-assisted CRISPR/Cas12a electrochemiluminescence (ECL) biosensor was developed for ultrasensitive detection of Staphylococcus aureus (Sa)-16S rDNA. The biosensor utilized a porphyrinic Zr metal-organic framework (MOF) as a coreactant accelerator to enhance the ECL emission signal. The presence of Sa-16S rDNA triggered the SDA reaction, generating single-stranded DNA products (SP) that activated the CRISPR/Cas12a trans-cleavage and effectively extinguished the ECL signals. The ECL platform exhibited high sensitivity and specificity, making it suitable for clinical diagnosis.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Qingxin Zhang, Manru Zhang, Zhiqiang Guo, Jingjing Li, Zhixue Zhu, Yu Wang, Su Liu, Jiadong Huang, Jinghua Yu
Summary: In this study, a low-background electrochemical biosensor based on a DNA tetrahedron-besieged primer and a DNAzyme-activated programmatic rolling circle amplification (RCA) was proposed for OTA detection in wine samples. The low-background detection was achieved by using the besieged primer and the target OTA-mediated DNAzyme activation initiated the programmatic RCA. The integration of the catalytic property of silver nanoclusters (AgNCs) with the electrochemical assay provided high sensitivity for OTA detection. This novel design showed great potential in food safety and environmental detection.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Xu Li, Jiali Huang, Jiayu Ding, Mingzhen Xiu, Kang Huang, Kang Cui, Jing Zhang, Shiji Hao, Yan Zhang, Jinghua Yu, Yizhong Huang
Summary: In this study, a dual-mode lab-on-paper device was constructed using BiVO4/FeOOH nanocomposites as an efficient photoelectrochemical (PEC)/colorimetric signal reporter for sensitive detection of carcinoembryonic antigen (CEA). The nanocomposites with an FeOOH needle-like structure displayed enhanced PEC response activity, enabling quantitative detection of CEA with the PEC signal readout mode. Additionally, a colorimetric signal readout system was achieved through a smart folding technique, providing a dual-mode signal readout for the lab-on-paper device.
Article
Chemistry, Analytical
Qian Wang, Liang Lv, Wenhao Chi, Yujiao Bai, Wenqing Gao, Peihua Zhu, Jinghua Yu
Summary: A new porphyrin-based porous covalent organic framework (TAP-COF) with high surface areas and efficient electron transport was synthesized. The TAP-COF exhibited excellent peroxidase-like catalytic activity and was able to catalyze the oxidation of a substrate by hydrogen peroxide. Simple and rapid colorimetric methods for in situ hydrogen peroxide detection were developed using this TAP-COF nanozyme, which has potential applications in point-of-care testing, cell biology, and clinical diagnosis.
Article
Chemistry, Analytical
Huihui Shi, Yanfei Che, Yumeng Rong, Jiajun Wang, Yanhu Wang, Jinghua Yu, Yan Zhang
Summary: An integrated dual-signal bioassay was developed for ultrasensitive detection of thrombin. This method combined visualization with photoelectrochemical technique to achieve the detection. The bioassay demonstrated wide linear ranges, high selectivity, stability, and reproducibility under optimized conditions. Additionally, the proposed visual/photoelectrochemical transduction mechanism platform offered a lower background signal and more reliable detection results, providing an effective way for detecting other proteins and nucleic acids.
Article
Chemistry, Analytical
Wenli Xiu, Peini Zhao, Yujie Pan, Xiaoru Wang, Lina Zhang, Shenguang Ge, Jinghua Yu
Summary: A surface-enhanced Raman scattering (SERS) sensor was developed for rapid on-site determination of ethephon in food, based on the folding and assembly characteristics of paper fibers, the skeleton controllability of metal-organic framework materials (MOFs), and the morphology designability of plasmonic noble metal materials. The sensor used HKUST-1(Cu) assembled onto a carbon-treated chromatographic paper matrix to overcome poor affinity of SERS substrate for target molecules. With the coupling of biomimetic antibodies and a multi-stage progressive enhancement structure, the sensor achieved high specificity and sensitivity for ethephon detection with a linear range of 10-3 to 10 mg kg-1 and a low detection limit of around 1.39 x 10-4 mg kg-1.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Li Shan, Yuanyuan Chen, Xiaoran Tan, Shenguang Ge, Lina Zhang, Lin Li, Jinghua Yu, Li Li
Summary: A newly designed two-in-one tetrahedral DNA (TDN) nanostructure with an antifouling surface and backbone-rigidified interfacial tracks was developed for highly sensitive and selective detection of miRNA-182-5p. The TDN-modified sensing interfaces demonstrated incomparable antifouling ability against protein samples and interfering miRNAs due to the strong hydrophilic capacity and special molecular conformations. The designed TDN-modified paper-based photoelectro-chemical sensor successfully quantified miRNA-182-5p with a low detection limit of 0.09 fM and high specificity in real samples, showing great promise for bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Physical
Shanshan Li, Haihan Yu, Yuwen Wang, Shuai Wang, Lina Zhang, Peihua Zhu, Chaomin Gao, Jinghua Yu
Summary: A stable organic-inorganic Z-scheme heterostructure was constructed by covalently connecting COF-366-Fe and CeO2 through Schiff base reaction, which effectively integrated their respective properties and improved the separation and transfer of photoexcited carriers. The COF-366-Fe@CeO2 exhibited superior photocatalytic CO yield compared to COF-366-Fe and CeO2, indicating its potential as a highly-effective COF-based photocatalyst for artificial photosynthesis.
APPLIED SURFACE SCIENCE
(2023)
Article
Biophysics
Lin Li, Hui Tian, Guofu Wang, Suyue Ren, Tinglei Ma, Yixiang Wang, Shenguang Ge, Yan Zhang, Jinghua Yu
Summary: Ready-to-use in vitro diagnosis is crucial for cervical cancer prevention and treatment. A paper-film-based differential lateral flow biosensor is proposed, allowing simultaneous detection of two high-risk HPV types. The biosensor utilizes a CuCo2S4/ZnIn2S4 heterostructure on a paper-film chip and introduces surface sulfur vacancy for enhanced photoexcitation. Through sequential solution transfer, the biosensor activates the self-assembly of DNA and the cleavage activity of CRISPR-Cas12a, resulting in the release of polydopamine-coated gold nanoparticles. This intelligent biosensor achieves low limit-of-detection for HPV-18 and HPV-16 and provides a promising approach for home medical intelligent diagnosis.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Chemistry, Physical
Yunqing Liu, Zuhao Zhang, Lu Zhang, Yuguo Xia, Haiqing Wang, Hong Liu, Shenguang Ge, Jinghua Yu
Summary: Integrating bifunctional electrocatalysts into one electrolytic cell for water electrolysis has demonstrated the convenience and efficiency in green hydrogen production. This study developed a dual metal doping strategy and successfully designed a high-performance bifunctional electrocatalyst for robust water splitting, achieving both oxygen evolution reaction and hydrogen evolution reaction at low overpotentials in alkaline media. The collaboration approach of codoped low-valence and high-valence metals shows great potential in the development of high-performance and versatile catalysts.
JOURNAL OF MATERIALS CHEMISTRY A
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
