期刊
BIOSENSORS & BIOELECTRONICS
卷 36, 期 1, 页码 6-11出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.02.064
关键词
Label-free immunosensor; Hollow nanostructure; Graphene; Carcinoembryonic antigen; Thionine
类别
资金
- Natural Science Foundation of China [21075052, 21175057, 51005103]
- Natural Science Foundation of Shandong Province [ZR2010BM030, ZR2010ZR063]
- Science and Technology Key Plan Project of Shandong Province [2010GSF10628]
- Special Research and Development Environmental Protection Industry of Shandong Province
- National Major Projects on Water Pollution Control and Management Technology [2008ZX07422]
- Science and Technology Development Plan Project of Jinan City [201004015]
Interests in using nanoporous metals for biosensing applications have been increasing. Herein, nanotubular mesoporous PdCu (NM-PdCu) alloy is used to fabricate a novel label-free electrochemical immunosensor for cancer biomarker carcinoembryonic antigen (CEA). It operates through physisorption of anti-CEA on NM-PdCu and the mixture of sulfonated graphene sheets (HSO3-GS) and thionine (TH) functionalized glassy carbon electrode interface as the detection platform. In this study, chitosan (CS)-PdCu is bound very strongly to carcinoembryonic antibody (anti-CEA), because of the good electron conductivity, high surface area, and good biocompatibility. CS-PdCu is immobilized on electrodes by electrostatic interactions between the negatively charged sulfo group of HSO3-GS and the abundant positively charged amino groups of chitosan. TH acts as the redox probe. Under the optimized conditions, the electrochemical immunosensor exhibits a wide working range from 0.01 to 12 ng/mL with a low detection limit of 4.86 pg/mL. The accuracy, reproducibility, and stability of the immunosensor are acceptable. The assay is evaluated for real serum samples, receiving satisfactory results. The nanoporous metal materials-based immunoassay provides a promising approach in clinical application and thus represents a versatile detection method. (C) 2012 Elsevier B.V. All rights reserved.
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