期刊
BIOSENSORS & BIOELECTRONICS
卷 34, 期 1, 页码 267-272出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.02.019
关键词
Silicon nanowire; Field-effect transistor; Biosensor; Cardiac troponin I; Acute myocardial infarction
类别
资金
- National Basic Research Program of China (973 Program) [2011CB965004, 2009CB930802]
- National Natural Science Foundation of China (NSFC) [10834004, 11104317]
- Chinese Academy of Sciences
A label-free biosensor for electrical detection of cardiac troponin I (cTnI), a highly sensitive and selective biomarker of acute myocardial infarction (AMI), is demonstrated using silicon nanowire (SiNW) based field-effect transistors (FETs). The FET devices were fabricated by a complementary metal oxide semiconductor (CMOS) compatible top-down approach to define the SiNW followed by tetramethylammonium hydroxide (TMAH) wet etching. Electrical characterizations of the SiNW FET revealed an ambipolar conduction characteristic with an on/off ratio of 10(5)-10(6). CTnI monoclonal antibodies were then covalently immobilized on the SiNW surfaces. By integrating with a homemade biosensor measurement system, the biosensor exhibited rapid and sensitive response to cTnI proteins. The current response showed a nature of logarithm relationship against the cTnI concentration from 46 ng/mL down to 0.092 ng/mL Moreover, an anti-interference capability of the fabricated biosensor was also assessed. By utilizing the top-down fabrication method, this work provides an efficient way for the cTnI proteins detection with an enormous potential of mass-production, which definitely facilitate the practical applications. (c) 2012 Elsevier B.V. All rights reserved.
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