Article
Electrochemistry
Kavita Jayakumar, Thomas M. B. Reichhart, Christopher Schulz, Roland Ludwig, Alfons K. G. Felice, Donal Leech
Summary: Cellobiose dehydrogenase (CDH) is a biocatalyst capable of oxidizing cellobiose and related carbohydrates to generate electrical current. A novel engineered CDH has shown improved performance as a bioelectrocatalyst in glucose biosensors.
Article
Chemistry, Analytical
Yaping Dong, Xiaojin Luo, Yiqun Liu, Cunling Yan, Haixia Li, Jicheng Lv, Li Yang, Yue Cui
Summary: A disposable creatinine biosensor, based on Prussian blue/carbon-graphite paste and immobilized enzymes, was developed for clinical evaluation of renal function detection. The biosensor showed a linear detection range and high stability, and yielded comparable results to the Jaffe method. This biosensor holds great potential for medical and healthcare applications.
Review
Engineering, Electrical & Electronic
Yaping Dong, Yiqun Liu, Jicheng Lv, Li Yang, Yue Cui
Summary: Kidney disease is a leading cause of death worldwide, and creatinine serves as a key biomarker for it. Amperometric biosensors have gained attention for their rapid, sensitive, and selective detection capabilities. The miniaturized and portable nature of these biosensors makes them popular for various settings. This review explores the development of amperometric biosensing for creatinine, including mechanisms, materials, applications, and commercial status, and can be a valuable resource for future creatinine biosensor development and practical applications in kidney patients.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2023)
Article
Chemistry, Analytical
Oana Maria Istrate, Lucian Rotariu, Camelia Bala
Summary: In this study, a new ethanol biosensor based on a modified electrode with poly(allylamine hydrochloride) and immobilized alcohol dehydrogenase in a sol-gel matrix was developed and optimized for parameters such as applied potential, pH, NAD(+) concentration, and storage conditions. The biosensor showed a wide response range from 0.05 to 2 mM with a high sensitivity of 13.45 ± 0.67 μA/mM•cm2 and a low detection limit of 20 μM. Furthermore, the developed biosensor demonstrated accurate detection of ethanol in commercial beverages.
Review
Chemistry, Analytical
Christopher J. Matthews, Emma S. V. Andrews, Wayne M. Patrick
Summary: This review systematically outlines the progress in enzyme-based amperometric biosensors for monitoring malic acid concentration, highlighting advancements in sensitivity and interference reduction. However, a trade-off between sensitivity and linear range is identified. Future research may focus on identifying or engineering superior alternatives to improve the commercial utility of malic acid biosensors.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Physical
Alfons K. G. Felice, Christian Schuster, Alan Kadek, Frantisek Filandr, Christophe V. F. P. Laurent, Stefan Scheiblbrandner, Lorenz Schwaiger, Franziska Schachinger, Daniel Kracher, Christoph Sygmund, Petr Man, Petr Halada, Chris Oostenbrink, Roland Ludwig
Summary: This study elucidates the role of the cytochrome (CYT) domain of cellobiose dehydrogenase (CDH) in two electron transfer steps, revealing key factors and protein conformational changes in interdomain electron transfer.
Article
Biophysics
Kouta Takeda, Ryo Kusuoka, Misaki Inukai, Kiyohiko Igarashi, Hiroyuki Ohno, Nobuhumi Nakamura
Summary: An amperometric L-fucose biosensor was developed using direct electron transfer bioelectrocatalysis, which allowed for accurate detection of L-fucose while eliminating interference from other electroactive compounds.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Multidisciplinary Sciences
Hucheng Chang, Neus Gacias Amengual, Alexander Botz, Lorenz Schwaiger, Daniel Kracher, Stefan Scheiblbrandner, Florian Csarman, Roland Ludwig
Summary: This study demonstrates the activity of Lytic polysaccharide monooxygenase (LPMO) on wood cell walls and suggests that even low levels of hydrogen peroxide can support the enzymatic degradation of wood biomass. LPMO plays a synergistic role in fungal extracellular catabolism and industrial biomass degradation.
NATURE COMMUNICATIONS
(2022)
Review
Biochemistry & Molecular Biology
Franziska Schachinger, Hucheng Chang, Stefan Scheiblbrandner, Roland Ludwig
Summary: Accurate determination of analyte concentrations is crucial for various applications; Enzyme-based biosensors offer fast, accurate quantification, easy use, and cost-effectiveness; This review focuses on enzymes capable of direct electron transfer to electrodes, presenting amperometric biosensors for medical, technical, and environmental analytes.
Article
Food Science & Technology
Hui Wu
Summary: This paper presents the development of an electrochemical sensor based on a modified ionic liquid for the detection of lactose content in dairy products. The sensor utilizes cellobiose dehydrogenase as the catalytic enzyme for lactose oxidation, exhibiting excellent electrocatalytic activity and enabling sensitive and selective lactose detection. The sensor offers a wide linear range and low detection limit, providing rapid analysis of lactose in dairy products without complex sample preparation steps.
JOURNAL OF FOOD MEASUREMENT AND CHARACTERIZATION
(2023)
Article
Chemistry, Analytical
Ademar Wong, Anderson M. Santos, Orlando Fatibello-Filho, Maria D. P. T. Sotomayor
Summary: A renewable tyrosinase-based biosensor for catechol detection was developed in this work, showing high accuracy and sensitivity in experiments, with successful application in river water samples. The biosensor is advantageous for its direct detection without the use of a mediator and its potential for immobilizing other enzymes and biomolecules with low-cost materials.
Article
Multidisciplinary Sciences
Anton A. Stepnov, Vincent G. H. Eijsink, Zarah Forsberg
Summary: Lytic polysaccharide monooxygenases (LPMOs) are mono-copper enzymes that catalyze oxidative depolymerization of recalcitrant substrates such as chitin or cellulose. Recent research has shown that LPMOs can catalyze fast peroxygenase reactions and that the presence of a cellulose-binding module (CBM) can affect the in situ production of H2O2. This study investigated the interplay between a CBM-containing LPMO variant and a truncated form without the CBM. The results demonstrate that truncation of the CBM leads to increased H2O2 production and decreased enzyme stability. Furthermore, the combination of the two enzyme forms results in synergistic effects, improving cellulose degradation while reducing enzyme inactivation caused by off-pathway reactions with excess H2O2.
SCIENTIFIC REPORTS
(2022)
Article
Engineering, Electrical & Electronic
Kazushi Suzuki, Hitoshi Muguruma, Hisanori Iwasa, Takeshi Tanaka, Atsunori Hiratsuka, Tetsuo Shimidu, Yohko Hoshino, Katsumi Tsuji, Takahide Kishimoto
Summary: We have developed an amperometric biosensor strip that can detect the ketone body 3-hydroxybutyrate (3HB) in serum. The strip has a small volume capillary chamber and uses carbon nanotubes (CNTs) and 3HB dehydrogenase (3HBDH) as the working electrode. The CNTs are well dispersed in an aqueous solution, and the sensor can detect 3HB in human serum within the physiological range of 0.05-4.4 mM with a response time of 15 seconds.
IEEE SENSORS JOURNAL
(2023)
Article
Biochemistry & Molecular Biology
Christopher J. Matthews, Wayne M. Patrick
Summary: This study describes a pipeline of database-mining, gene synthesis, recombinant expression, and spectrophotometric assays to develop a biocatalyst, AsME(R181Q), for l-malate biosensors. The biosensor has an ultra-wide linear range and sensitivity, making it suitable for detecting l-malate in fruit juices and wines.
Article
Biochemistry & Molecular Biology
Christopher J. Matthews, Wayne M. Patrick
Summary: This article describes a pipeline for evaluating previously untested enzymes for their suitability in biosensors, resulting in the development of a bespoke biocatalyst AsME(R181Q). The biosensor has a tunable combination of sensitivity and linear range, allowing for measurement of L-malate concentrations in undiluted fruit juices and wines, with potential applications in the food and beverage industry.
Article
Agriculture, Multidisciplinary
Kei Kumakura, Chiaki Hori, Hiroki Matsuoka, Kiyohiko Igarashi, Masahiro Samejima
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2019)
Article
Physics, Multidisciplinary
Takahiro Ezaki, Katsuhiro Nishinari, Masahiro Samejima, Kiyohiko Igarashi
PHYSICAL REVIEW LETTERS
(2019)
Review
Biochemistry & Molecular Biology
Kouta Takeda, Kiyohiko Igarashi, Makoto Yoshida, Nobuhumi Nakamura
BIOELECTROCHEMISTRY
(2020)
Article
Biotechnology & Applied Microbiology
Jorg C. de Ruijter, Kiyohiko Igarashi, Merja Penttila
FEMS YEAST RESEARCH
(2020)
Article
Multidisciplinary Sciences
Taku Uchiyama, Takayuki Uchihashi, Akihiko Nakamura, Hiroki Watanabe, Satoshi Kaneko, Masahiro Samejima, Kiyohiko Igarashi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2020)
Article
Biochemistry & Molecular Biology
Robert Pylkkanen, Pezhman Mohammadi, Suvi Arola, Jorg C. de Ruijter, Naoki Sunagawa, Kiyohiko Igarashi, Merja Penttila
Article
Biochemistry & Molecular Biology
Kaori Matsuyama, Naomi Kishine, Zui Fujimoto, Naoki Sunagawa, Toshihisa Kotake, Yoichi Tsumuraya, Masahiro Samejima, Kiyohiko Igarashi, Satoshi Kaneko
JOURNAL OF BIOLOGICAL CHEMISTRY
(2020)
Article
Materials Science, Paper & Wood
Tomohiro Kuga, Naoki Sunagawa, Kiyohiko Igarashi
Summary: This study synthesized cellulose in space to investigate the role of gravity in the formation of cellulose crystals and highly ordered structures. The results showed that gravity played a significant role in cellulose II crystal sedimentation and the building of network structure, and cellulose synthesized in space had a more uniform particle distribution compared to cellulose synthesized on the ground.
Article
Biochemistry & Molecular Biology
Keisuke Kojima, Naoki Sunagawa, Nils Egil Mikkelsen, Henrik Hansson, Saeid Karkehabadi, Masahiro Samejima, Mats Sandgren, Kiyohiko Igarashi
Summary: In this study, two xylan enzymes were compared to gain insights into their structures and catalytic mechanisms. The results showed that the two enzymes exhibited different degradation efficiencies and specificities towards xylooligosaccharides of different lengths. Additionally, it was found that wood-decaying fungi and molds use different enzymes for xylan degradation, possibly due to their distinct environmental niches.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2022)
Article
Biochemistry & Molecular Biology
Keisuke Kojima, Naoki Sunagawa, Yoshihisa Yoshimi, Theodora Tryfona, Masahiro Samejima, Paul Dupree, Kiyohiko Igarashi
Summary: This study compares the activity of two types of xylanases in degrading acetylated and deacetylated xylan. The results suggest that the degradability of xylan by GH11 xylanases is highly dependent on the extent of acetyl group substitution. Additionally, the study found a potential coevolution relationship between GH11 xylanases and CE1 AXEs.
JOURNAL OF APPLIED GLYCOSCIENCE
(2022)
Article
Multidisciplinary Sciences
Taku Uchiyama, Takayuki Uchihashi, Takuya Ishida, Akihiko Nakamura, Josh V. Vermaas, Michael F. Crowley, Masahiro Samejima, Gregg T. Beckham, Kiyohiko Igarashi
Summary: Efficient depolymerization of crystalline cellulose requires the cooperative action of multiple cellulolytic enzymes. This study demonstrates that the synergistic activity between cellobiohy-drolases and a lytic polysaccharide monooxygenase can enhance the activity and performance of the enzymes by producing chain breaks and amorphizing the crystalline surface of cellulose.
Article
Biochemistry & Molecular Biology
Takahiro Fujii, Kiyohiko Igarashi, Masahiro Samejima
JOURNAL OF APPLIED GLYCOSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Sora Yamaguchi, Naoki Sunagawa, Mikako Tachioka, Kiyohiko Igarashi, Masahiro Samejima
JOURNAL OF APPLIED GLYCOSCIENCE
(2020)
Article
Biochemistry & Molecular Biology
Kiyohiko Igarashi, Satoshi Kaneko, Motomitsu Kitaoka, Masahiro Samejima
JOURNAL OF APPLIED GLYCOSCIENCE
(2020)
Review
Materials Science, Paper & Wood
Chiaki Hori, Makoto Yoshida, Kiyohiko Igarashi, Masahiro Samejima
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)