期刊
BIOSENSORS & BIOELECTRONICS
卷 38, 期 1, 页码 132-137出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2012.05.014
关键词
Electrochemical immunosensor; Nonspecific adsorption; Signal amplification; Graphene oxide nanosheet; Bionanoconjugates; Cancer biomarker
类别
资金
- ARC DCECRA [DE120102503]
- UQ [RM 201000124]
- National Breast Cancer Foundation [CG-08-07]
An electrochemical immunoassay which minimizes nonspecific protein adsorption and improves detection sensitivity of proteomic cancer biomarker is described. Our technique comprises two novel features: (i) a high density terminally functionalized poly(N-isopropyl acrylamide) 'brush' layer is grown by surface initiated reversible addition fragmentation chain transfer (RAFT) polymerization method from the electrode surface in order to minimize nonspecific adsorption of serum proteins and other biomolecules, and (ii) a signal amplifying 'bionanoconjugate' comprised of graphene oxide nanosheets decorated with CdSe quantum dots and recombinant single-chain variable fragments towards MSLN, is used to 'physically' amplify the anodic stripping voltammetric signal. This method enabled a detection limit of ca. 1 pg/mL MSLN (RSD = 4.6%, n=4) spiked in serum samples. Because of the simple, specific and sensitive nature of this methodology, we feel that it may find potential use in serum-based protein diagnostics. (C) 2012 Elsevier B.V. All rights reserved.
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