期刊
BIOSENSORS & BIOELECTRONICS
卷 28, 期 1, 页码 386-392出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.07.053
关键词
Conductive polymer; PEDOT:TsO; Electrochemical impedance spectroscopy (EIS); Human cytomegalovirus (CMV); Human fibroblast cells; Atomic force microscopy (AFM); Scanning ion conductance microscopy (SICM)
类别
资金
- Danish Research Council for Technology and Production Sciences
- Technical University of Denmark
The demand in the field of medical diagnostics for simple, cost efficient and disposable devices is growing. Here, we present a label free, all-polymer electrochemical biosensor for detection of acute viral disease. The dynamics of a viral infection in human cell culture was investigated in a micro fluidic system on conductive polymer PEDOT:TsO microelectrodes by electrochemical impedance spectroscopy and video time lapse microscopy. Employing this sensitive, real time electrochemical technique, we could measure the immediate cell response to cytomegalovirus. and detect an infection within 3 h, which is several hours before the cytopathic effect is apparent with conventional imaging techniques. Atomic force microscopy and scanning ion conductance microscopy imaging consolidate the electrochemical measurements by demonstrating early virus induced changes in cell morphology of apparent programmed cell death. (C) 2011 Elsevier B.V. All rights reserved.
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