期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 7, 页码 3297-3302出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.01.001
关键词
Polyaniline nanowire; Biosensing; Immunoglobulin G; Myoglobin; Surface immobilization
类别
资金
- National Science Foundation (NSF) [ECCS 0824035]
- National Institutes of Health (NIH) [NIH 1R21EB008825]
- Div Of Electrical, Commun & Cyber Sys
- Directorate For Engineering [0824035] Funding Source: National Science Foundation
A single polyaniline (PANI) nanowire-based biosensor was established to detect immunoglobulin G (IgG) and myoglobin (Myo), which is one of the cardiac biomarkers. The single PANI nanowires were fabricated via an electrochemical growth method, in which single nanowires were formed between a pair of patterned electrodes. The single PANI nanowires were functionalized with monoclonal antibodies (mAbs) of IgG or Myo via a surface immobilization method, using 1-ethyl-3-(3-dimethyaminopropyl) carbodiimide (EDC), and N-hydroxysuccinimde (NHS). The functionalization was then verified by Raman spectroscopy and fluorescence microscopy. The target proteins of IgG and Myo were detected by measuring the conductance change of functionalized single PANI nanowires owing to the capturing of target proteins by mAbs. The detection limit was found to be 3 ng/mL for IgG and 1.4 ng/mL for Myo. No response was observed when single nanowires were exposed to a non-specific protein, demonstrating excellent specificity to expected target detection. Together with the fast response time (a few seconds), high sensitivity, and good specificity, this single PANI nanowire-based biosensor shows great promise in the detection of cardiac markers and other proteins. (C) 2011 Elsevier B.V. All rights reserved.
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