期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 7, 页码 3175-3180出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.12.023
关键词
Electrochemiluminescence; Peroxydisulfate solution; L-Cysteine; alpha-1-Fetoprotein; Immunosensor
类别
资金
- National Natural Science Foundation (NNSF) of China [21075100]
- Ministry of Education of China [708073]
- Natural Science Foundation of Chongqing City [CSTC-2009BA1003]
- State Key Laboratory of Electroanalytical Chemistry [SKLEAC 2010009]
- Foundation of Southwest University, China [XSGX02]
- Southwest China University [KB2010006]
A novel label free electrochemiluminescence (ECL) immunosensor based on the ECL of peroxydisulfate solution for detection of alpha-1-fetoprotein (AFP) has been developed. For this proposed immunosensor, L-cysteine was firstly electrodeposited on the gold electrode surface, which promoted the electron transfer and largely enhanced the ECL of peroxydisulfate solution. Subsequently, gold nanoparticles (nano-Au) were assembled onto the L-cysteine film modified electrode to improve the absorption capacity of antibody and further amplify the ECL signal. Then, antibody was immobilized onto the electrode through nano-Au. At last bovine serum albumin (BSA) was employed to block the nonspecific binding sites. As a result, a novel ECL immunosensor was firstly obtained by applying the ECL of peroxydisulfate solution without conventional luminescent reagents. The AFP was determined in the range of 0.01-100 ng mL(-1), with a low detection limit of 3.3 pg mL(-1) (S/N =3). The proposed ECL immunosensor provides a rapid, simple, and sensitive immunoassay protocol for protein detection, which might hold a promise for clinical application. Moreover, this work would open up a new field in the application of peroxydisulfate solution ECL for highly sensitive bioassays. (C) 2010 Elsevier B.V. All rights reserved.
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