期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 8, 页码 3608-3613出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.02.012
关键词
MWNTs-Ru(bpy)(3)(2+) composite; Solid-state electrochemiluminescence; Glucose-dehydrogenase; Wild type p53 sequence; Muted type p53 sequence
类别
资金
- National Nature Science Foundation of China [20675031]
- National innovation experimental project of China [101028642]
- Southeast University [3225000501]
- Pre-Research Foundation of Southeast University [KJ2010489]
An enzyme-based solid-state electrochemiluminescence (ECL) sensing platform for sensitive detection of a single point mutation is developed successfully using p53 tumor suppressor gene as a model analyte. A composite of multiwalled carbon nanotubes and Ruthenium (II) tris-(bipyridine)(MWNTs-Ru(bpy)(3)(2+)) was prepared and coated on an electrode surface, which was covered by polypyrrole (PPy) to immobilize ssDNA. Then, the ssDNA recognized the gold nanoparticle (AuNP)-labeled p53 tumor suppressor gene, and produced AuNP-dsDNA electrode with AuNP layer. The surface adsorbed the glucose-dehydrogenase (GDH) molecules for producing ECL signal. This system combined enzyme reaction with ECL detection, and it can recognize sequence-specific wild type p53 sequence (wtp53) and muted type p53 sequence (mtp53) with discrimination of up to 56.3%. The analytic results were sensitive and specific. It holds promise for the diagnosis and management of cancer. (C) 2011 Elsevier B.V. All rights reserved.
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