期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 12, 页码 4815-4818出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.04.019
关键词
Solid-state electrochemiluminescence; In-situ; Ru-PtNPs; Streptavidin-alkaline phosphatase; conjugate; Aptasensor
类别
资金
- NNSF of China [21075100]
- Ministry of Education of China [708073]
- Chongqing Key Laboratory on Luminescence and Real-Time Analysis [CSTC-2006CA8006]
- Natural Science Foundation of Chongqing [CSTC-2010BB4121, CSTC-2009BA1003]
- Fundamental Research Funds for the Central Universities [XDJK2010C062, XDJK2009B013]
- High Technology Project Foundation of Southwest University [XSGX02]
- State Key Laboratory of Electroanalytical Chemistry, China [SKLEAC2010009]
Herein, an ultrasensitive solid-state tris(2,2'-bipyridyl) ruthenium(II) (Ru(bpy)(3)(2+)) electrochemiluminescence (ECL) aptasensor using in-situ produced ascorbic acid as coreactant was successfully constructed for detection of thrombin. Firstly, the composite of Ru(bpy)(3)(2+) and platinum nanoparticles (Ru-PtNPs) were immobilized onto Nafion coated glass carbon electrode, followed by successive adsorption of streptavidin-alkaine phosphatase conjugate (SA-ALP) and biotinylated anti-thrombin aptamer to successfully construct an ECL aptasensor for thrombin determination. In our design, Pt nanoparticles in Ru(bpy)(3)(2+)-Nafion film successfully inhibited the migration of Ru(bpy)(3)(2+) into the electrochemically hydrophobic region of Nafion and facilitated the electron transfer between Ru(bpy)(3)(2+) and electrode surface. Furthermore, ALP on the electrode surface could catalyze hydrolysis of ascorbic acid 2-phosphate to in-situ produce ascorbic acid, which co-reacted with Ru(bpy)(3)(2+) to obtain quite fast, stable and greatly amplified ECL signal. The experimental results indicated that the aptasensor exhibited good response for thrombin with excellent sensitivity, selectivity and stability. A linear range of 1 x 10(-15)-1 x 10(-8) M with an ultralow detection limit of 0.33 fM (S/N =3 ) was obtained. Thus, this procedure has great promise for detection of thrombin present at ultra-trace levels during early stage of diseases. (C) 2011 Elsevier B.V. All rights reserved.
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据