期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 10, 页码 4241-4244出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.04.004
关键词
Rapid detection; Strip; DNA detection; Bacterial fruit blotch; Plant bacterium
类别
资金
- National Natural Science Foundation of China [21071066, 20835006, 91027038]
- special fund for inspection and quarantine scientific Research in the Public Interest [200810517]
- special Fund for Agroscientific Research in the Public Interest [201003066]
Acidovorax avenae subsp. citrulli (AAC) is one of the most harmful diseases in cucurbit production. A rapid and sensitive DNA strip sensor was constructed based on gold nanoparticle-labeled oligonucleotide probes for the detection of AAC. Both the qualitative and semi-quantitative detections of target DNA were successfully achieved using the developed DNA strip sensor. The qualitative limit of detection CLOD) of the strip sensor was determined as 4 nM. The LOD for the semi-quantitative detection was calculated to be 0.48 nM in the range of 0-10 nM. The genomic DNA was detected directly using the DNA strip sensor without any further treatment. This DNA strip sensor is a potentially useful tool for rapid on-site DNA screening. (C) 2011 Elsevier B.V. All rights reserved.
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