4.8 Article

Toward immunoassay chips: Facile immobilization of antibodies on cyclic olefin copolymer substrates through pre-activated polymer adlayers

期刊

BIOSENSORS & BIOELECTRONICS
卷 26, 期 9, 页码 3967-3972

出版社

ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2011.03.005

关键词

Amphiphilic polymers; Cyclic olefin copolymer; Polymer adlayers; Anti-biofouling; Antibody immobilization; Immunoassay

资金

  1. ETRI, Republic of Korea [A1100-0802-0112]
  2. MKE/KEIT [10031930]
  3. Korea Evaluation Institute of Industrial Technology (KEIT) [10031930] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)

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Our research efforts have focused on the surface modification and the potential as a biochip material of commercially available cyclic olefin copolymer (COC) substrates. For the surface modification of hydrophobic COC substrates we synthesized two types of amphiphilic polymers having three important functions: hydrophobic (dodecyl or benzyl) groups, serving to anchor the COC substrate; a PEG component, which acts as a repellent of non-specific biomolecules; and an NHS ester group for conjugation of biomolecules. Formation of the polymer adlayers on COC surface was confirmed using a contact angle analyzer. The anti-biofouling property of the polymer-coated COC surface was examined by measuring the extent of nonspecific adsorption of immunoglobulin G (IgG), resulting in a very low level of the protein adsorption compared to uncoated COC surfaces (control). In addition, antibodies, used as representative biomolecules for immunoassay, could be selectively immobilized on the polymer-coated COC surface. Sandwich immunoassay using anti-rabbit IgG immobilized COC surface showed linear response to rabbit IgG of model analyte over a range from 1 to 1000 ng/mL with detection limit of 1-10 ng/mL. We also fabricated a Lab-On-a Chip type of COC biochip, which could detect a cardiac marker protein, troponin I (TnI), with detection limit of 10 ng/mL. (C) 2011 Elsevier B.V. All rights reserved.

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