Article
Chemistry, Analytical
Xuan Wang, Yuanlong Qin, Xinyu Zhang, Yumin Leng, Zhengbo Chen
Summary: An electrochemical aptasensor was constructed using Au/TiO2 nanorod arrays for ultrasensitive detection of adenosine. The aptasensor showed linear correlation between the peak SWV current values and the logarithmic concentration of adenosine, with a minimum detection limit of 0.42 fM. The aptasensor exhibited high specificity, precision, stability, and applicability for adenosine, providing a promising clinical diagnostic tool for adenosine detection.
MICROCHEMICAL JOURNAL
(2023)
Article
Biophysics
Cong Han, Wenjin Li, Qian Li, Wenping Xing, Hang Luo, Haishuo Ji, Xiaona Fang, Zhaofeng Luo, Liyun Zhang
Summary: In this study, a CRISPR/Cas12a-derived electrochemical aptasensor for fast and ultrasensitive detection of COVID-19 nucleocapsid protein (Np) was developed. The sensor exhibited high selectivity to Np and could complete a test within 30 minutes. It showed great potential in early diagnosis of COVID-19, environmental surveillance, and food security.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Yawei Wang, Lei Chen, Tiantian Xuan, Jian Wang, Xiuwen Wang
Summary: An integrated electrode system consisting of a graphene working electrode, a carbon counter electrode, and an Ag/AgCl reference electrode was fabricated for label-free detection of carcinoembryonic antigen. The linear relationship between CEA concentration (0.2-15.0 ng/ml) and impedance change was demonstrated, with a calculated detection limit of 0.085 ng/ml.
FRONTIERS IN CHEMISTRY
(2021)
Article
Chemistry, Analytical
Wan Huang, Yue Zhou, Danyan Zhan, Guosong Lai
Summary: A novel biosensing method for ultrasensitive electrochemical detection of kanamycin antibiotic was developed by inducing the assembled formation of DNA nanostructures at an electrode. Dual signal amplification assisted by Exo I and HRP led to a very low detection limit of 9.1 fg mL(-1) for Kana assay.
ANALYTICA CHIMICA ACTA
(2021)
Article
Chemistry, Analytical
Junfeng Zhang, Kai Sun, Jiangtao Ren, Han Wang, Jing Cheng
Summary: A metallic nanowire aptasensor with high sensitivity and short detection time was developed for the early diagnosis and prognostic monitoring of heart failure by detecting cardiac troponin I (cTnI). The sensor exhibited excellent detection performance, including high sensitivity, short detection time, high specificity, and good reproducibility, making it a potential application in portable devices for continuous monitoring of cardiomyocyte injury.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Nanoscience & Nanotechnology
Xiaoyan He, Jiandi Dong, Huimin Han, Nan Sun, Wenyu Shi, Xiong Lu, Hui Jia, Xiaoquan Lu
Summary: The newly designed electrochemical aptasensor utilizing DNA-templated copolymers is capable of sensitive detection of ATP, with a wide linear response range and high selectivity, achieving a detection limit as low as 11.5 fM.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Article
Biophysics
Shuqing Huang, Zhenping Liu, Yu Yan, Jiamei Chen, Ruizhi Yang, Qiuju Huang, Mingliang Jin, Lingling Shui
Summary: This study presents the development of an ultrasensitive electrochemical aptasensor for the detection of trace CRP molecules in plasma. The sensor shows excellent sensing performance with a broad linear dynamic range and a low limit of detection.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Analytical
Seyed Milad Taghdisi Heidarian, Ashraf Tavanaee Sani, Mohammad Danesh, Mohammad Ramezani, Mona Alibolandi, Golara Gerayelou, Khalil Abnous, Seyed Mohammad Taghdisi
Summary: This study developed a novel electrochemical detection strategy using a double-labelled aptamer and complementary strands, demonstrating excellent sensitivity and selectivity towards fluoroquinolones. The method showed simplicity and successfully detected ciprofloxacin in spiked real samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Analytical
Beenish Noureen, Najeeb Ullah, Yulan Tian, Liping Du, Wei Chen, Chunsheng Wu, Ping Wang
Summary: This study developed an electrolyte-insulator semiconductor (EIS) sensor for the label-free detection of saxitoxin (STX), one of the most dangerous shellfish toxins. The sensor was covered with a layer of positively-charged weak polyelectrolyte and utilized a specific aptamer for STX detection. The results showed that the aptasensor had a low detection limit and good selectivity and stability. This sensor provides a convenient approach for the moderate, rapid, and label-free detection of marine biological toxins.
Article
Chemistry, Analytical
Hema Bhardwaj, Gajjala Sumana, Christophe A. Marquette
Summary: This study reports the development of an electrical and optical biosensor using gold nanobipyramids for label-free detection of Aflatoxin B-1. The sensor showed high sensitivity and robustness, and was successfully applied in detecting AFB-1 in maize samples.
Article
Biochemical Research Methods
Canan Ozyurt, Inci Uludag, Mustafa Kemal Sezginturk
Summary: This study developed a disposable electrochemical aptasensor based on ITO-PET film for sensitive and selective detection of prostate-specific antigen (PSA) in real serum samples. The biosensor exhibited excellent sensitivity, precision, repeatability, and storage stability, making it a potential candidate for clinical applications.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Jintao Liang, Fei Guo, Jiaying Huang, Xiaohong Tan, Lixian Sun, Zhide Zhou, Guiyin Li
Summary: An ultrasensitive label-free electrochemical aptasensing platform was constructed for the detection of GP73 protein in hepatocellular carcinoma.
MICROCHEMICAL JOURNAL
(2023)
Article
Chemistry, Analytical
Joohyung Park, Minwoo Kim, Woochang Kim, Seongjae Jo, Woong Kim, Chihyun Kim, Hyunjun Park, Wonseok Lee, Jinsung Park
Summary: We developed a novel electrochemical aptasensor for the quantitative detection of 25-hydroxy vitamin D-3 in saliva, which is highly selective and ultra-sensitive.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Wei Li, Ding Zhao, Dongdong Tian, Manman Zhai, Hongdan Xu, Liuchuang Zheng, Shengqiang Li, Ye Sang
Summary: The article introduces an enzyme-free electrochemical strategy for analyzing thrombin concentration in human serum using a DNA network assembled by nonlinear hybridization chain reaction. The strategy has high sensitivity and specificity, and performs well in detecting thrombin in serum samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Chemistry, Analytical
Rongrong Yuan, Zhuojun Yan, Alateng Shaga, Hongming He
Summary: A novel porous organic polymer (POP) with high surface area and stability was successfully prepared and used to fabricate an ultrasensitive electrochemical aptasensor for detecting trace ampicillin. The biosensor exhibited a low detection limit and accurate monitoring ability for detecting amp in multiple real samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2021)
Article
Chemistry, Multidisciplinary
Beijia Cao, Hongyu Zhang, Maozhong Sun, Chuanlai Xu, Hua Kuang, Liguang Xu
Summary: A novel probe based on chiral molybdenum diselenide nanoparticles modified by cyanine 3 is developed for the detection of reactive oxygen species (ROS). The probe shows decreased circular dichroism (CD) signals and recovered fluorescence in the presence of ROS. With high selectivity and sensitivity, the probe can detect ROS in living cells and monitor ROS levels in vivo. This strategy offers a new approach for ROS detection and inspires investigations on chiral nanomaterials as biosensors.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Aihua Qu, Qiwen Chen, Maozhong Sun, Liguang Xu, Changlong Hao, Chuanlai Xu, Hua Kuang
Summary: Excessive accumulation of ROS can cause oxidative stress and damage, leading to aging and age-related diseases. This study introduces MnO2 nanoparticles that can monitor and eliminate ROS in real-time, providing a new strategy for the diagnosis and treatment of age-related diseases.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Analytical
Qi Chen, Li Yao, Jianguo Xu, Qiujing Qi, Sha Tao, Xin Song, Wei Chen
Summary: In this study, ultrasensitive and non-invasive screening of MOP residues in fingerprint sweat was achieved by stepwise Au decoration-assisted double signal amplification and antibody-saving strategies on LFS. This approach has great potential in the ultrasensitive screening of trace analytes in various fields and widens the application scope of lateral flow strips.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Shiyi Wang, Ziwen Zong, Jianguo Xu, Bangben Yao, Zhou Xu, Li Yao, Wei Chen
Summary: In this study, a visual strategy for rapid and ultrasensitive detection of OTA was proposed by integrating primer-mediated exponential rolling circle amplification (P-ERCA) with a designed nucleic acid lateral flow strip (LFS). This method demonstrates highly sensitive detection of OTA and could be utilized as a universal method for detection of other chemical analytes.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Jiarong Cai, An-An Liu, Xue-Hui Shi, Haohao Fu, Wei Zhao, Liguang Xu, Hua Kuang, Chuanlai Xu, Dai-Wen Pang
Summary: In this study, a novel methodology for synthesizing chiral CdSe@ZnS quantum dots (QDs) with enhanced circularly polarized luminescence (CPL) was developed. The resulting chiral QDs exhibit exceptional photoluminescence quantum yield and chiroptical activities. Investigations of the synthetic pathway reveal the importance of the interface as a binary synthetic environment in facilitating ligand exchange and shell growth, leading to maximum fluorescent brightness and chiral effects.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Food Science & Technology
Hanling Wang, Xianzhuo Meng, Li Yao, Qian Wu, Bangben Yao, Zhaoran Chen, Jianguo Xu, Wei Chen
Summary: Meat adulteration-based food fraud is a significant concern globally, and a microarray chip polymerase chain reaction (PCR) directed microfluidic lateral flow strip (LFS) device has been developed to accurately identify beef adulterated with chicken, duck, and pork in processed beef products. This device offers flexible combination and integration of sample loading, detection, and reporting, allowing individual visualization of DNA amplicons on the LFS unit for species identification and quantification. The proposed method and device have been proven practical, accurate, and reliable in identifying adulterants in ground meat samples.
FOOD CHEMISTRY: MOLECULAR SCIENCES
(2023)
Article
Chemistry, Multidisciplinary
Xiaoqian Jiang, Aihua Qu, Xinxin Xu, Hua Kuang, Liqiang Liu, Liguang Xu, Chuanlai Xu
Summary: A hapten containing a benzene ring and an amino group was used to screen a monoclonal antibody (mAb) 3G10 with high specificity and sensitivity for the detection of free Valproic acid (VPA) in human serum. A colloidal gold-based immunochromatographic strip (CG-ICS) was developed with gold-labeled mAb 3G10 for qualitative and quantitative analysis of VPA. The CG-ICS showed good correlation with a direct chemiluminescence kit, making it suitable for rapid clinical monitoring of free VPA.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Chunhao Wei, Lingling Guo, Aihong Wu, Chuanlai Xu, Hua Kuang, Xinxin Xu, Liqiang Liu
Summary: A simple, fast, and suitable colloidal gold immunochromatographic detection method for common epidemic strains of Norovirus was established, with good specificity and stability. The minimum detection concentration of this method is 10 ng/mL, allowing for rapid and accurate on-site detection.
NEW JOURNAL OF CHEMISTRY
(2023)
Article
Cell Biology
Xiao Guo, Chen Li, Jia Zhang, Maozhong Sun, Jun Xu, Chuanlai Xu, Hua Kuang, Liguang Xu
Summary: Oral administration of chiral nanoparticles can improve cognitive abilities and reduce pathological features in Alzheimer's disease mice, potentially achieved by altering gut microbiota composition and increasing gut metabolite indole-3-acetic acid levels.
Review
Nanoscience & Nanotechnology
Guangbo Yu, Aihua Qu, Zhimeng Wu, Liguang Xu, Chuanlai Xu, Hua Kuang
Summary: This article introduces the progress in the application of chiral nanomaterials in the field of bioscience, including the strategies for constructing different types of ROS-related chiral nanomaterials and the biological effects achieved by regulating ROS using chiral nanomaterials. The key issues and future prospects of chiral nanomaterials in the regulation of ROS are also discussed.
Review
Chemistry, Multidisciplinary
Changlong Hao, Chuanlai Xu, Hua Kuang
Summary: This article provides an overview of recent advances in chiral nanomaterials for biosensing applications, including individual chiral nanoparticles, chiral assemblies, and chiral 2D film-based sensors.
CHEMICAL COMMUNICATIONS
(2023)
Article
Chemistry, Physical
Xinxin Xu, Aihong Wu, Lingling Guo, Hua Kuang, Liguang Xu, Chuanlai Xu, Liqiang Liu
Summary: In this study, a gold nanoparticle-based immunochromatographic assay was developed for the rapid detection of IMZ in grapes and tomatoes, demonstrating excellent practicality.
NANOSCALE HORIZONS
(2023)
Article
Food Science & Technology
Yang Liu, Liqiang Liu, Maozhong Sun, Xinxin Xu, Hua Kuang, Chuanlai Xu, Liguang Xu
Summary: In this study, a novel hapten scheme was designed to prepare a monoclonal antibody for the simultaneous detection of common gibberellins (GAs) with high sensitivity and homogeneity. Then, a colloidal gold immunochromatographic assay (GICA) was established using synthetic gold nanoparticles, which simplifies the sample pre-treatment step. The method showed rapid, sensitive, and efficient on-site detection of GAs in food.
Article
Chemistry, Applied
Qianqian Lu, Hongliu Ding, Liqiang Liu, Liguang Xu, Hua Kuang, Chuanlai Xu, Lingling Guo
Summary: An immunochromatographic assay was developed for the rapid and efficient screening of FPF residues in grapes, blueberries, and tomatoes. The assay showed high sensitivity and specificity, with detection limits of 0.009 mg/kg, 0.033 mg/kg, and 0.040 mg/kg for grapes, blueberries, and tomatoes, respectively. The results obtained from the assay were consistent with those obtained from LC-MS/MS analysis.
Article
Chemistry, Applied
Zhou Xu, Yanqiu Chen, Rong Wang, Maolong Chen, Jian Zhang, Yunhui Cheng, Bangben Yao, Li Yao, Jianguo Xu, Wei Chen
Summary: In this study, nanoporous-carbon-Fe3O4 (NPC-Fe3O4) composites were prepared using metal-organic framework materials. The composites showed excellent antimatrix effect and significantly improved relaxivity, making them suitable for detecting aflatoxin B1 in complex food matrix.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
