期刊
BIOSENSORS & BIOELECTRONICS
卷 25, 期 6, 页码 1509-1515出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.11.011
关键词
Microfluidic flow cytometer; Particle-based assays; Cell analysis; Polymeric gel electrode; Impedance detection; Fluorescence detection
类别
资金
- Ministry of Education, Science and Technology (MEST) [M10536090001-05N3609-00110]
- MKE (Ministry of Knowledge Economy), Korea
- NIPA (National IT Industry Promotion Agency) [NIPA 2009-(C1090-0902-0002)]
- National Research Foundation of Korea [2005-2001287] Funding Source: Korea Institute of Science & Technology Information (KISTI), National Science & Technology Information Service (NTIS)
A portable microfluidic flow cytometer with dual detection ability of impedance and fluorescence was developed for cell analysis and particle-based assays. In the proposed system, fluorescence from microparticles and cells is measured through excitation by a light emitting diode (LED) and detection by a solid-stated photomultiplier (SSPM). Simultaneous impedometric detection provides information on the existence and size of microparticles and cells through polyelectrolyte gel electrodes (PGEs) operated by custom designed circuits for signal detection, amplification, and conversion. Fluorescence and impedance signals were sampled at 1 kHz with 12 bit resolution. The resulting microfluidic cytometer is 15 x 10 x 10 cm(3) in width, depth, and height, with a weight of about 800 g. Such a miniaturized and battery powered system yielded a portable microfluidic cytometer with high performance. Various microbeads and human embryonic kidney 293 (HEK-293) cells were employed to evaluate the system. Impedance and fluorescence signals from each bead or cell made classification of micro particles or cells easy and fast. (c) 2009 Elsevier B.V. All rights reserved
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