期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 2, 页码 418-423出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.07.112
关键词
Electrochemical immunosensor; Seed-mediated deposition; Pt nanocatalyst; Prostate-specific antigen
类别
资金
- Institute of Bioengineering and Nanotechnology (Biomedical Research Council, Agency for Science, Technology and Research, Singapore)
Herein we describe a novel signal amplification strategy for the development of ultrasensitive electrochemical immunosensors. The amplification strategy is based on platinum catalyzing a hydrogen evolution reaction. To demonstrate its practicality, the electrochemical signal enhancement strategy has been applied for the development of a novel prostate-specific antigen (PSA) immunosensor. The immunosensing protocol utilized a gold electrode with PSA capture antibodies bound to its surface via covalent bonding. After PSA was bound to the electrode surface, a secondary platinum nanoparticle-labeled detection antibody was used to complete the sandwich immunosensor. The resulting electrode was then dipped in a platinum developer solution containing 1 mM of PtCl42-, 0.1 M of formate (reductant) and 0.5% Tween 80 (pH 6.5) to generate bare platinum catalysts in close proximity to the Au electrode surface through a seed-mediated nucleation and growth mechanism. The signal readout was obtained electrochemically via a Pt-catalyzed hydrogen evolution reaction in an acidic aqueous medium containing 10 mM of HCl and 1 M of KCl. A detection limit of 1 fg/ml was achieved. (C) 2010 Elsevier B.V. All rights reserved.
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