期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 2, 页码 760-765出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.06.043
关键词
Molecular imprinting; Protein imprinting; Surface imprinting; Nanoparticles; Lysozyme
类别
资金
- National Natural Science Foundation of China [20574038]
- Natural Science Foundation of Tianjin [09JCYBJC02900]
Surface imprinting and adopting a nano-sized physical form are two effective approaches to overcome the template transfer difficulty within molecularly imprinted polymers and in particular advantageous to the imprinting of macromolecular structures like proteins. The surface protein-imprinted nanoparticles based on these two strategies are attractive for biosensor development. We here demonstrate a facile way for imprinting protein over nanoparticle supports. It was achieved simply via radical induced graft copolymerization of low concentration monomers on the surface of vinyl modified silica nanoparticles dispersed in aqueous media with lysozyme as a model protein. With a total monomer concentration of 0.4 wt%, less than tenth that employed conventionally, the possible gelation of the dispersion after polymerization was avoided and hence the unagglomerated imprinted particles could be readily collected. It was proved that thin polymer shells with imprinted sites had been formed over the support particles. In batch rebinding tests, the imprinted particles reached saturated adsorption within 5 min and exhibited significant specific recognition toward the template protein. The presented approach may be a versatile way for the fabrication of surface protein-imprinted nanoparticles via rational design of the surface chemistry of the support particles and choice of functional monomers according to the properties of the print protein. (C) 2010 Elsevier B.V. All rights reserved.
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