Article
Immunology
Sebastien Breurec, Thierry Franck, Elisabeth Njamkepo, Jean-Robert Mbecko, Jean Rauzier, Hugues Sanke-Waigana, Guyguy Kamwiziku, Renaud Piarroux, Marie-Laure Quilici, Francois-Xavier Weill
Summary: Four cholera outbreaks were reported in the Central African Republic during 1997-2016, caused by Vibrio cholerae O1 serotype Inaba from 3 seventh pandemic El Tor sublineages originating from West Africa or the African Great Lakes Region.
EMERGING INFECTIOUS DISEASES
(2021)
Article
Immunology
Yaovi M. G. Hounmanou, Elisabeth Njamkepo, Jean Rauzier, Karin Gallandat, Aurelie Jeandron, Guyguy Kamwiziku, Klaudia Porten, Francisco Luquero, Aaron Aruna Abedi, Baron Bashige Rumedeka, Berthe Miwanda, Martin Michael, Placide Welo Okitayemba, Jaime Mufitini Saidi, Renaud Piarroux, Francois-Xavier Weill, Anders Dalsgaard, Marie-Laure Quilici
Summary: Lake Tanganyika basin in Africa is a hotspot for cholera. Vibrio cholerae O1 isolates collected from the Democratic Republic of the Congo side of the lake during 2001-2020 belonged to 2 out of the 5 clades of the AFR10 sublineage. One clade became dominant after acquiring a parC mutation that reduced susceptibility to ciprofloxacin.
EMERGING INFECTIOUS DISEASES
(2023)
Article
Environmental Sciences
Eizo Takahashi, Kei Kitahara, Shin-ichi Miyoshi, Goutam Chowdhury, Asish K. Mukhopadhyay, Shanta Dutta, Sadayuki Ochi, Keinosuke Okamoto
Summary: Many cholera patients are found in Kolkata, India every year, indicating the widespread presence of cholera toxin-producing Vibrio cholerae O1. Suitable conditions for the replication of toxigenic V. cholerae O1 are provided in Kolkata. A study showed that the replication rate of toxigenic V. cholerae O1 is low in low ionic aqueous solution. The ion concentration in the environmental water of Kolkata was found to be significantly high, suggesting the suitability of Kolkata's environmental water for the survival of V. cholerae O1.
ENVIRONMENTAL RESEARCH
(2023)
Article
Biotechnology & Applied Microbiology
Ghazale Faridfar, Mehdi Zeinoddini, Saeid Akbarzedehkolahi, Shahin Faridfar, Afshin Samimi Nemati
Summary: The newly developed LSPR biosensor can be used for simple, sensitive, and reliable detection of V. cholerae O1 (Inaba strain), providing high sensitivity analysis of cholera bacteria through nanobioprobes and monoclonal antibodies. This sensor helps prevent the spread of cholera disease.
INTERNATIONAL MICROBIOLOGY
(2021)
Article
Immunology
Nabila Benamrouche, Chafika Belkader, Elisabeth Njamkepo, Sarah Sihem Zemam, Soraya Sadat, Karima Saighi, Dalila Torkia Boutabba, Faiza Mechouet, Rym Benhadj-Slimani, Fatma-Zohra Zmit, Jean Rauzier, Farid Kias, Souad Zouagui, Corinne Ruckly, Mohamed Yousfi, Amel Zertal, Ramdane Chouikrat, Marie-Laure Quilici, Francois-Xavier Weill
Summary: After a lull of more than 20 years, Algeria experienced a cholera outbreak in 2018 with 291 suspected cases. The outbreak strains were identified as Vibrio cholerae O1 serotype Ogawa from the seventh pandemic El Tor sublineage AFR14, indicating a new introduction of cholera into Africa from South Asia.
EMERGING INFECTIOUS DISEASES
(2022)
Article
Microbiology
Murari Bhandari, Irani U. Rathnayake, Flavia Huygens, Son Nguyen, Brett Heron, Amy V. Jennison
Summary: This study characterized the genetic features of Vibrio cholerae O1 isolates from Australia and compared them with international isolates. The findings showed limited genetic relatedness between Australian and international strains, indicating different lineages. Understanding the origin of Australian strains and devising control measures are important for reducing Vibrio cholerae exposure.
MICROBIOLOGY SPECTRUM
(2023)
Article
Immunology
Pan Lu, Jialiang Chen, Zhenpeng Li, Zhe Li, Jingyun Zhang, Biao Kan, Bo Pang
Summary: In this study, a CARID system was developed for rapid detection of toxigenic V. cholerae serogroups. The system showed rapid, sensitive, economically efficient, and portable features, and was suitable for detecting complex samples.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2022)
Article
Infectious Diseases
Bibhuti Bhusan Pal, Ashish Kumar Nayak, Smruti Ranjan Nayak
Summary: This study identified variants of the ctxB allele of Vibrio cholerae O1 isolated in Odisha, India between 1995 and 2019. Different genotypes dominated in different time periods, potentially impacting the spread of the disease.
INTERNATIONAL JOURNAL OF INFECTIOUS DISEASES
(2021)
Article
Microbiology
Yun Luo, Michael Payne, Sandeep Kaur, Sophie Octavia, Jianmin Jiang, Ruiting Lan
Summary: This study sequenced and compared non-pandemic Vibrio cholerae O1 isolates from Zhejiang, China with publicly available non-pandemic O1 genomes. The isolates were classified into three lineages, with one lineage sharing the most recent common ancestor with the pandemic clones. Each lineage had unique properties contributing to their disease-causing capacity. The findings provide new insights into the evolution of V. cholerae O1 for cholera prevention and control.
MICROBIOLOGY SPECTRUM
(2023)
Article
Environmental Sciences
Ashish Kumar Nayak, Smruti Ranjan Nayak, Dipti Ranjan Behera, Bibhuti Bhusan Pal
Summary: The study analyzed the antimicrobial susceptibility, virulence genes, and drug resistance genes of Vibrio cholerae O1 strains isolated from outbreaks and epidemics in Odisha, India over a period of two and a half decades. The findings showed that 90% of the isolates carried antibiotic drug resistant genes, and indicated the origin and spread of the Haitian variant tcpA in V. cholerae O1 strains.
ENVIRONMENTAL MICROBIOLOGY REPORTS
(2021)
Article
Infectious Diseases
John Mwaba, Amanda K. Debes, Kelsey N. Murt, Patrick Shea, Michelo Simuyandi, Natasha Laban, Katayi Kazimbaya, Caroline Chisenga, Shan Li, Mathieu Almeida, Jacquelyn S. Meisel, Aaron Shibemba, Timothy Kantenga, Victor Mukonka, Geoffrey Kwenda, David A. Sack, Roma Chilengi, O. Colin Stine
Summary: Genetic analysis revealed three separate transmission events of Vibrio cholerae in Zambia, suggesting the presence of both random mutation and alternative mechanisms of nucleotide variation. It is recommended to implement targeted intervention strategies locally and well-coordinated plans regionally to control future cholera outbreaks effectively.
BMC INFECTIOUS DISEASES
(2021)
Article
Microbiology
Tania Nasreen, Nora A. S. Hussain, Jia Yee Ho, Vanessa Zhi Jie Aw, Munirul Alam, Stephanie K. Yanow, Yann F. Boucher
Summary: This article describes a method for rapid detection of Vibrio cholerae in water samples without the need for DNA extraction. It is useful for field research in low-resource settings and can support continuous monitoring in cholera-endemic areas.
Article
Microbiology
Kaoru Ochi, Tamaki Mizuno, Prosenjit Samanta, Asish K. Mukhopadhyay, Shin-ichi Miyoshi, Daisuke Imamura
Summary: Cholera, caused by pathogenic strains of V. cholerae generated by lysogenization of the filamentous cholera toxin phage CTX Phi, has recently shown alterations in the CTX Phi prophage array, resulting in an inability to replicate the genome. This change suggests a significant shift in the dissemination pattern of the current cholera pandemic.
Article
Food Science & Technology
Qingzhen Miao, Wen Ding, Xiuli Bao, Siyuan Wang, Qianru Lin, Yingying Xu, Jing Lu, Mingsheng Lyu, Shujun Wang
Summary: In this study, specific DNAzymes of Vibrio cholerae (Vc) were successfully identified through nine rounds of in vitro selection using an unmodified DNA library. A DNAzyme (DVc1) with good activity and specificity was selected, with a detection limit of 7.2 x 10(3) CFU/mL of Vc. A simple biosensor was constructed by immobilizing DVc1 and its substrate in shallow circular wells of a 96-well plate. The sensor effectively detected Vc in aquatic products within 20 minutes, showcasing its simplicity and efficiency. This sensitive DNAzyme sensor can serve as a rapid onsite Vc detection tool.
FOOD SCIENCE & NUTRITION
(2023)
Article
Ecology
Tania Nasreen, Mohammad Tarequl Islam, Kevin Y. H. Liang, Fatema-Tuz Johura, Paul C. Kirchberger, Eric Hill, Marzia Sultana, Rebecca J. Case, Munirul Alam, Yann F. Boucher
Summary: Cholera caused by Vibrio cholerae has been prevalent in the Ganges Delta for centuries. A study conducted in Dhaka found that the abundance and distribution of V. cholerae lineages in surface water are influenced by environmental factors, with salinity, conductivity, and total dissolved solids positively correlated with intraspecies richness. The dominance of certain lineages can change due to fluctuations in environmental factors like salinity, affecting competition dynamics among the lineages.
Article
Chemistry, Physical
Chatwarin Poochai, Chakrit Sriprachuabwong, Jedsada Sodtipinta, Jaruwit Lohitkarn, Patiya Pasakon, Vitsarut Primpray, Nattida Maeboonruan, Tanom Lomas, Anurat Wisitsoraat, Adisorn Tuantranont
Summary: The synthesized alpha-MnO2/N&S-rGO composite demonstrated promising electrochemical performance in supercapacitor electrodes, exhibiting high specific capacitance, energy density, and power density. Additionally, the composite showed good electrochemical stability and long cycle life, making it a potential candidate for high-performance ionic liquid-based quasi solid-state supercapacitors.
JOURNAL OF COLLOID AND INTERFACE SCIENCE
(2021)
Article
Chemistry, Analytical
Kreeta Sukthang, Jantana Kampeera, Chakrit Sriprachuabwong, Wansika Kiatpathomchai, Eakkachai Pengwang, Adisorn Tuantranont, Wishsanuruk Wechsatol
Summary: In this study, EWOD fabrication with suitable materials was proposed for creating lab-on-a-chip platforms for shrimp EMS diagnosis. Qualitative sensitivity for detecting EMS DNA plasmid concentration using the colorimetric LAMP-XO technique on the EWOD platform was confirmed through comparison with standard laboratory tests.
Article
Physics, Multidisciplinary
Sarunrit Kesornkhup, Adisorn Tuantranont, Tanom Lomas, Chakrit Sriprachuabwong, Kamol Wasapinyokul
Summary: The study examined the accuracy of the Swanepoel method in calculating the thickness of spin-coated polymer films, revealing that films with higher solution concentrations or slower spin-coating speeds tend to be thicker. Errors between Swanepoel and actual thickness were mainly influenced by film surface roughness.
ACTA PHYSICA POLONICA A
(2021)
Article
Chemistry, Physical
Nantikan Tammanoon, Anurat Wisitsoraat, Adisorn Tuantranont, Chaikarn Liewhiran
Summary: This study investigates the sensitivity and selectivity of Zn-substituted SnO2 nanoparticles towards formic acid, demonstrating their potential for ultrasensitive and selective detections of HCOOH in practical applications. The results show that a sensing layer with the optimum Zn content of 0.2 wt% provided a remarkably high response towards 1000 ppm HCOOH, indicating a significant improvement in performance compared to undoped samples. Additionally, good selectivity against various volatile organic compounds was achieved, highlighting the promising applications of the flame-made Zn-substituted SnO2 sensors.
JOURNAL OF ALLOYS AND COMPOUNDS
(2021)
Article
Chemistry, Analytical
Suphatsorn Thimoonnee, Kanpitcha Somnet, Pattanun Ngaosri, Sanoe Chairam, Chanpen Karuwan, Wichayaporn Kamsong, Adisorn Tuantranont, Maliwan Amatatongchai
Summary: We report a new ready-to-use sensor for simultaneous determination of paraquat (PQ) and glyphosate (GLY) based on a graphite screen-printed electrode modified with a dual-molecularly imprinted polymer coated on a mesoporous silica-platinum core. The sensor shows good linear calibration curves and high selectivity, making it suitable for simultaneous detection of PQ and GLY concentrations in water samples.
ANALYTICAL METHODS
(2022)
Article
Electrochemistry
Wichayaporn Kamsong, Vitsarut Primpray, Patiya Pasakon, Chakrit Sriprachuabwong, Saithip Pakapongpan, Johannes Philipp Mensing, Anurat Wisitsoraat, Adisorn Tuantranont, Chanpen Karuwan
Summary: A highly sensitive and disposable screen-printed ionic liquid-graphene electrode (SPIL-GE) was developed for electrochemical sensing. The electrode showed improved electrochemical performance compared to other types of electrodes, making it a potential candidate for advanced electrochemical sensing applications.
ELECTROCHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Physical
Johannes Philipp Mensing, Tanom Lomas, Adisorn Tuantranont
Summary: In this study, high performance LICs were successfully developed using rGO@Pd and activated carbon materials, demonstrating excellent electrochemical performance and cycle life.
Article
Chemistry, Inorganic & Nuclear
Surangkana Wannapop, Anuwat Khawsaad, Apisit Supanpong, Yupin Janorat, Yaowamarn Chuminjak, Adisorn Tuantranont, Anukorn Phuruangrat, Titipun Thongtem, Somchai Thongtem, Asanee Somdee
Summary: In this study, the role of p-n junction in the photocatalytic degradation of dye-contaminated wastewater was investigated by synthesizing n-type ZnO enhanced with metal oxides like CuO, NiO, and Fe2O3. The performance of CuO/ZnO and NiO/ZnO materials was found to be better compared to pure ZnO, while Fe2O3/ZnO exhibited decreased photocatalytic activity. Ni(5)-ZnO showed the highest performance, degrading dyes by over 99.71% in contaminated water.
INORGANIC CHEMISTRY COMMUNICATIONS
(2022)
Article
Chemistry, Analytical
Pakapreud Khumwan, Sukanya Pengpanich, Jantana Kampeera, Wichayaporn Kamsong, Chanpen Karuwan, Assawapong Sappat, Prapaporn Srilohasin, Angkana Chaiprasert, Adisorn Tuantranont, Wansika Kiatpathomchai
Summary: We developed a LAMP-based technique that utilizes primers with base mismatches to specifically detect isoniazid resistance in tuberculosis. This technique demonstrates high sensitivity and specificity, and can be performed on a handheld potentiostat device for endpoint detection.
MICROCHEMICAL JOURNAL
(2022)
Article
Chemistry, Analytical
Saithip Pakapongpan, Yingyot Poo-arporn, Adisorn Tuantranont, Rungtiva P. Poo-arporn
Summary: In this study, a simple one-pot solvothermal synthesis approach was used to fabricate a magnetic iron oxide embed nitrogen-doped graphene nanohybrid. This nanohybrid was then applied in an electrochemical sensor for the ultrasensitive detection of antibiotic residue contamination in milk samples. The sensor demonstrated excellent performance with high sensitivity, selectivity, and fast detection.
Article
Food Science & Technology
Nattanicha Chaiya, Donraporn Daranarong, Patnarin Worajittiphon, Runglawan Somsunan, Puttinan Meepowpan, Adisorn Tuantranont, Nawasit Rakbamrung, Paul D. D. Topham, Brian J. J. Tighe, Anisa Mahomed, Winita Punyodom
Summary: This study aimed to improve the properties of poly(lactic acid) (PLA) by melt blending with non-toxic poly(ethylene oxide) (PEO), and fabricate a biodegradable humidity sensing substrate using solvent-cast 3D printing. The results showed that the PLA/PEO blend exhibited enhanced flexibility and thermal stability, along with increased hydrophilicity with higher PEO content.
FOOD PACKAGING AND SHELF LIFE
(2022)
Article
Chemistry, Physical
Johannes Philipp Mensing, Tanom Lomas, Adisorn Tuantranont
Summary: Rechargeable magnesium batteries (RMBs) have the potential to contribute to the shift towards renewable and green energy sources, but still face challenges that need to be overcome. Graphene is being extensively studied for its ability to improve the performance of RMBs by enhancing electrochemical characteristics and mechanical stability of battery components.
Article
Polymer Science
Manasanan Namhongsa, Donraporn Daranarong, Robert Molloy, Sukunya Ross, Gareth M. Ross, Adisorn Tuantranont, Dheerawan Boonyawan, Jiraporn Tocharus, Sivanan Sivasinprasasn, Paul D. Topham, Brian J. Tighe, Winita Punyodom
Summary: In this study, two-component scaffolds consisting of a 3D-printed component and an electrospun fiber component were fabricated and treated with N-2-Ar plasma to enhance their surface properties. The plasma treatment significantly improved the surface roughness, wettability, and hydrophilicity of the scaffolds. The 3D-printed component provided mechanical strength, while the electrospun fiber component promoted cell attachment and proliferation. The designed scaffolds showed potential for supporting cell regeneration in biomedical applications.
EUROPEAN POLYMER JOURNAL
(2023)
Article
Nanoscience & Nanotechnology
Tanaporn Kongthong, Chatwarin Poochai, Chakrit Sriprachuabwong, Adisorn Tuantranont, Suwat Nanan, Nonglak Meethong, Pasit Pakawatpanurut, Taweechai Amornsakchai, Jedsada Sodtipinta
Summary: This study investigates a composite material consisting of porous, N-doped activated carbon derived from pineapple leaf fiber (PALF-NAC) and alpha-MnO2 nanofibers as an electrode material for high-performance supercapacitors (SCs). The PALF-NAC and alpha-MnO2 /PALF-NAC possess mesoporous structures with high specific surface areas. The alpha-MnO2/PALF-NAC electrode exhibits high specific capacitance and excellent cycling stability, making it a promising candidate for stable, cost-effective, and high-performance supercapacitors.
JOURNAL OF SCIENCE-ADVANCED MATERIALS AND DEVICES
(2022)
Article
Chemistry, Analytical
Kanpitcha Somnet, Suphatsorn Thimoonnee, Chanpen Karuwan, Wichayaporn Kamsong, Adisorn Tuantranont, Maliwan Amatatongchai
Summary: A novel electrochemical sensor based on a screen-printed graphene paste electrode modified with platinum nanoparticles and coated with a molecularly imprinted polymer was proposed for sensitive and cost-effective detection of paraquat herbicide. The sensor showed high sensitivity, selectivity, and a lower detection limit for quantification of paraquat residue on vegetables and fruits. The ready-to-use MIP sensor offers an alternative approach for food safety applications.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)
