期刊
BIOSENSORS & BIOELECTRONICS
卷 26, 期 1, 页码 139-143出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2010.05.023
关键词
PPO; Biosensor; Transketolase; Tyrosine; Layered double hydroxides; Amperometry
类别
资金
- Ministerio de Educacion de Espana
This paper proposes a new concept of transketolase (TK) activity profiling A tyrosinase (PPO) biosensor, based on the immobilization of this enzyme in a Mg2Al-Cl layered double hydroxide, was developed for the amperometric detection of N-acetyl-L-tyrosine ethyl ester monohydrate (N-Ac-Tyr-OEt) at -0 2V This compound was released during an enzymatic reaction catalyzed by TK with N-acetyl-O-(2R, 3S, 5-trihydroxy-4-oxopentyl)-L-tyrosine ethyl ester used as donor substrate. This tyrosinase biosensor was optimized for the detection of TK activity, including PPO optimum substrate concentration, electrolyte nature, pH, and influence of bovine serum albumin (BSA). It was found that N-Ac-Tyr-OEt release is dependent on TK concentration (U/mL) in the electrolyte medium These results demonstrate the sensitivity and specificity of the tyrosinase biosensor designed for in vitro detection of TK activity, which is known to be involved in several diseases (C) 2010 Elsevier B V All rights reserved
作者
我是这篇论文的作者
点击您的名字以认领此论文并将其添加到您的个人资料中。
推荐
暂无数据