Article
Chemistry, Medicinal
Guo-Fang Duan, Yang Liu, Li-Na Zhang, Hong-Ye Li, Jie-Sheng Liu, Wei-Dong Yang
Summary: The study found that cinnamaldehyde can effectively reduce the accumulation of diarrhetic shellfish toxins in Perna viridis and decrease the histological alterations caused by DSTs in the mussel's digestive gland. Furthermore, cinnamaldehyde may decrease DSTs accumulation by suppressing CYP3A4 induction.
Article
Biochemical Research Methods
Hamid Aghamohammadi, Kathryn E. Thomas, Sanjana Srikant, Jason Deglint, Alexander Wong, Mahla Poudineh
Summary: To address the need for rapid detection of cyanotoxins in water resources, an easy-to-use and miniaturized system was developed. A bead-based, competitive fluorescence assay was presented for multiplexed detection of microcystin-LR (MC-LR) and okadaic acid (OA). A reusable microfluidic device, called toxin-chip, was designed for automated detection, which included a micromixer for efficient mixing and a detection chamber for magnetic retainment of beads. Quantum dots (QDs) were used as reporter molecules to enhance sensitivity, and the emitted fluorescence signal was inversely proportional to the toxin concentration. The device showed a low detection limit for both MC-LR and OA, and demonstrated chemical specificity against potential interfering toxins. The toxin-chip holds promise as a versatile and simple tool for cyanotoxin detection.
Article
Chemistry, Analytical
Yuxiang Ji, Gaozhe Cai, Cheng Liang, Zehang Gao, Weimin Lin, Zizhen Ming, Shilun Feng, Hongwei Zhao
Summary: To realize rapid, simple, and accurate detection of Okadaic acid (OA) in shellfish, a novel microfluidic immunosensor based on magnetic beads modified with a highly specific and sensitive monoclonal antibody (mAb) against OA was developed and used in conjunction with smartphone imaging. The method achieves on-site detection results within 1 h with an IC50 value of 3.30 ng/mL for OA and a limit of detection (LOD) of 0.49 ng/mL. Real sample analysis showed good accuracy and precision, and the results were reported in a specially designed smartphone app. The microfluidic immunosensor provides a reliable technical solution for detecting OA residues in shellfish, with the advantages of simple operation, rapid detection, and high sensitivity.
ANALYTICA CHIMICA ACTA
(2023)
Article
Chemistry, Analytical
Xiaotong Li, Yongqiang Cheng, Ranran Xu, Ziwei Zhang, Xiaoxiao Qi, Longyu Chen, Meijia Zhu
Summary: In this study, a smartphone-assisted microarray immunosensor combined with an indirect competitive ELISA was developed for quantitative colorimetric detection of Okadaic acid (OA). A novel graphene oxide composite probe was used to improve the detection sensitivity. The system showed a wide linear range for OA detection with low detection limit and good applicability in shellfish samples.
Article
Biochemical Research Methods
Elliott J. Wright, Pearse McCarron
Summary: A freeze-dried mussel tissue-certified reference material containing a complex profile of marine algal toxins was analyzed using liquid chromatography-high-resolution mass spectrometry. Over one hundred toxin analogues were identified, including previously unreported compounds, through a series of targeted and untargeted MS/MS experiments.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Marine & Freshwater Biology
Nancy I. Lewis, Rencheng Yu, Cheryl Rafuse, Michael A. Quilliam
Summary: A three-year field study was conducted in a mussel aquaculture site in Ship Harbour, Nova Scotia, Canada to detect toxic phytoplankton species and dissolved lipophilic phycotoxins and domoic acid. Plankton monitoring and SPATT techniques were used for analysis. The results showed seasonal changes in toxin composition were related to specific phytoplankton cell concentrations. SPATT samplers deployed within the mussel farm detected lower concentrations of phycotoxins compared to those deployed outside the farm.
Article
Food Science & Technology
Simone Balbinot, Anand Mohan Srivastav, Jasmina Vidic, Ibrahim Abdulhalim, Marisa Manzano
Summary: This article highlights the importance and advantages of optical biosensors in food safety, comparing them with traditional methods and showcasing the applications of different technologies and future trends.
TRENDS IN FOOD SCIENCE & TECHNOLOGY
(2021)
Article
Chemistry, Physical
Huimin Nai, Lixin Cao, Shuai Sun, Rujin Li, Haiping Liu, Peisheng Yan
Summary: An ultrasensitive label-free electrochemical immunosensor for detecting okadaic acid (OA) was developed using p-aminobenzoic acid-modified gold three-dimensional nanoelectrode ensembles (3DNEEs). The sensor showed a high detection accuracy and a wide linear range for OA in oyster samples, demonstrating its potential application value in food safety and environmental monitoring.
Article
Chemistry, Analytical
Jie Zhou, Xueliang Wang, Jiajie Chen, Youjun Zeng, Dayong Gu, Bruce Zhi Gao, Yonghong Shao
Summary: In this study, a high sensitivity SPRi-based immunosensor was developed for the detection of hemoglobin. The immunosensor offers a simple, rapid, high-throughput, and sensitive approach for occult blood detection.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Food Science & Technology
Xiaomei Wang, Chen Yang, Wei Jiang, Manyu Zhang, Ruifang Li, Yajuan Lin, Quan Wang
Summary: A rapid and quantitative immunochromatographic assay for the detection of diarrhetic shellfish poisons was developed using lanthanide fluorescent microspheres. The test strip exhibited high accuracy and sensitivity, making it suitable for rapid quantitative on-site detection of OA in seafood.
Article
Chemistry, Organic
Humberto J. Dominguez, David Cabrera-Garcia, Cristina Cuadrado, Antonello Novelli, M. Teresa Fernandez-Sanchez, Jose J. Fernandez, Antonio Hernandez Daranas
Summary: The research determined the relative configuration of Prorocentroic acid and its effects on murine cortical neurons. Long-term exposure to subtoxic concentrations caused significant reorganization of neuronal signaling, potentially through the activation of plasticity processes.
Article
Chemistry, Medicinal
Yang Liu, Siyuan Xu, Qiudie Cai, Dawei Li, Hongye Li, Weidong Yang
Summary: In this study, we investigated the interaction between okadaic acid (OA) and gut microbiota using an in vitro fermentation experiment. We found that OA can reshape the bacterial composition and lead to the production of various metabolites. Our findings confirmed the direct interaction between OA and gut bacteria, providing valuable evidence for understanding the metabolic process and complex toxicity of OA.
Article
Green & Sustainable Science & Technology
Fabrizio Capoccioni, Laura Bille, Federica Colombo, Lidia Contiero, Arianna Martini, Carmine Mattia, Riccardo Napolitano, Nicolo Tonachella, Marica Toson, Domitilla Pulcini
Summary: Harmful dinoflagellate blooms in the Mediterranean Sea have increased, posing a risk to shellfish farming. This study developed a machine learning predictive model using oceanographic and monitoring data to predict okadaic acid bioaccumulation in Mediterranean mussels. The model achieved 82% accuracy and could be used to establish an online early warning system for shellfish farmers to increase sustainability and reduce product losses.
Article
Toxicology
Yang Liu, Yang Lu, Yu-Hu Jiao, Da-Wei Li, Hong-Ye Li, Wei-Dong Yang
Summary: In this study, the effects of Okadaic acid (OA) on the metabolism of gut bacteria in rats were analyzed using a multi-omics strategy and rRNA high-throughput technology. The study found that OA had significant effects on gut bacteria, causing changes in dominant genera and bacterial function genes. It was also discovered that OA could be transformed into other metabolites, such as dinophysistoxin-2 (DTX-2), in the gut. The findings provide important insights into the complex toxicity of OA and its environmental fate.
ARCHIVES OF TOXICOLOGY
(2022)
Correction
Chemistry, Analytical
Ana Castanheira, Marilia Barreiros dos Santos, Laura Rodriguez-Lorenzo, Raquel Queiros, Begona Espina
Summary: The study introduces a novel microfluidic system for sensitive and cost-effective detection of okadaic acid in mussels.
Article
Chemistry, Analytical
Rinku Chhasatia, Martin J. Sweetman, Beatriz Prieto-Simon, Nicolas H. Voelcker
SENSORS AND ACTUATORS B-CHEMICAL
(2018)
Article
Chemistry, Multidisciplinary
Keying Guo, Apoorva Sharma, Rou Jun Toh, Eva Alvarez de Eulate, Thomas R. Gengenbach, Xavier Ceto, Nicolas H. Voelcker, Beatriz Prieto-Simon
ADVANCED FUNCTIONAL MATERIALS
(2019)
Article
Chemistry, Multidisciplinary
Nekane Reta, Andrew Michelmore, Christopher P. Saint, Beatriz Prieto-Simon, Nicolas H. Voelcker
Article
Chemistry, Multidisciplinary
Gayathri Rajeev, Allison J. Cowin, Nicolas H. Voelcker, Beatriz Prieto Simon
FRONTIERS IN CHEMISTRY
(2019)
Article
Chemistry, Multidisciplinary
Muamer Dervisevic, Maria Alba, Li Yan, Mehmet Senel, Thomas R. Gengenbach, Beatriz Prieto-Simon, Nicolas H. Voelcker
Summary: The microneedle array-based transdermal sensing system offers a pain-free alternative for continuous glucose monitoring of interstitial fluid. The system demonstrates good selectivity and sensitivity, with accurate correlation to blood glucose levels measured by a commercial glucometer.
ADVANCED FUNCTIONAL MATERIALS
(2022)
Article
Electrochemistry
Clara Perez-Rafols, Keying Guo, Maria Alba, Rou Jun Toh, Nuria Serrano, Nicolas H. Voelcker, Beatriz Prieto-Simon
Summary: The study demonstrated the use of carbon-stabilized porous silicon (pSi) as a voltammetric sensing platform capable of discriminating the detection of various electroactive species with similar redox potentials. By studying the electrochemical performance of carbonized silicon in the detection of three dihydroxybenzene isomers, it was shown that carbonized silicon outperformed a traditional glassy carbon electrode in terms of electrochemical properties.
ELECTROCHIMICA ACTA
(2021)
Article
Biophysics
Muamer Dervisevic, Maria Alba, Timothy E. Adams, Beatriz Prieto-Simon, Nicolas H. Voelcker
Summary: Electrochemical devices for transdermal monitoring show great potential for diagnosing diseases such as Cancer, especially breast cancer, by selectively capturing and quantifying key biomarkers like ErbB2. The high-density gold coated silicon microneedle arrays exhibit linear response over a wide concentration range and may pave the way for high-performing wearable point-of-care devices.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Nanoscience & Nanotechnology
Keying Guo, Maria Alba, Grace Pei Chin, Ziqiu Tong, Bin Guan, Michael J. Sailor, Nicolas H. Voelcker, Beatriz Prieto-Simon
Summary: This study explores layered carbon-stabilized pSi nanostructures as an electrochemical biosensor, demonstrating its excellent electrochemical properties and high sensitivity for DNA detection. The biosensor achieves a detection limit of 0.4 pM for a 28-nucleotide DNA sequence, two orders of magnitude lower than any other pSi-based electrochemical DNA sensors reported to date.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Microbiology
Zane Vitenberga-Verza, Mara Pilmane, Ksenija Serstnova, Ivars Melderis, Lukasz Gontar, Maksymilian Kochanski, Andzelika Drutowska, Gergely Maroti, Beatriz Prieto-Simon
Summary: This study aimed to investigate the inflammatory and regulatory cytokines in the milk of dairy cows with subclinical and clinical mastitis. The results showed that IL-1 alpha, IL-4, and IL-17A drive the inflammatory responses in mastitis cases. Furthermore, the host defense response in mastitis is characterized by the continuation or resolution of the initial inflammation.
Article
Chemistry, Analytical
Amane Shiohara, Christopher D. Easton, Beatriz Prieto-Simon, Nicolas H. Voelcker
Summary: The authors developed a bioreceptor-based biosensor with tunable nanochannels for the size-selective detection of intact viruses. The sensor demonstrated the ability to differentiate non-infectious viral particles from intact/infectious viruses with high sensitivity and low detection limit.
Article
Chemistry, Analytical
Antonio Lazaro, Ramon Villarino, Marc Lazaro, Nicolau Canellas, Beatriz Prieto-Simon, David Girbau
Summary: This study investigates the feasibility of using a battery-less NFC potentiostat for the next generation of electrochemical point-of-care sensors, presenting a design based on an NFC microchip and a custom potentiostat. The prototype shows excellent agreement with measurements obtained from a commercial glucometer.
Article
Pharmacology & Pharmacy
Carlota Roca, Yunuen Avalos-Padilla, Beatriz Prieto-Simon, Valentin Iglesias, Miriam Ramirez, Santiago Imperial, Xavier Fernandez-Busquets
Summary: The methyl erythritol phosphate (MEP) pathway is crucial for malaria parasites and human pathogenic bacteria. A DNA aptamer (D10) has been developed to target the enzyme DXR in this pathway, showing high selectivity and specificity for the detection of bacterial and parasitized cells. This study suggests the potential of DNA aptamers as ligands for future therapeutic and diagnostic applications.
Review
Chemistry, Analytical
Antonio Lazaro, Ramon Villarino, Marc Lazaro, Nicolau Canellas, Beatriz Prieto-Simon, David Girbau
Summary: This article reviews recent advances in batteryless NFC sensors for chemical and biosensing. The availability of low-cost NFC ICs and their integration in smartphones have sparked interest in new batteryless NFC sensors. The review compares NFC energy harvesting to other technologies, discusses power transfer, and presents the state of the art in NFC-based chemical and biosensors.
Article
Chemistry, Analytical
Xavier Ceto, Jacqui M. McRae, Agnieszka Mierczynska-Vasilev, Nicolas H. Voelcker, Beatriz Prieto-Simon
Summary: This study aims to develop an easy-to-use sensor device for the detection of haze-forming proteins in wine. Both impedimetric immunosensors and FT-IR-based methods were explored, allowing for rapid assessment and quantification of protein content in wine.
Article
Chemistry, Multidisciplinary
Maria Alba, Morgane Robin, Donna Menzies, Thomas R. Gengenbach, Beatriz Prieto-Simon, Nicolas H. Voelcker
CHEMICAL COMMUNICATIONS
(2019)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)