Article
Chemistry, Multidisciplinary
Yin Bao, Kaiwei Cai, Yichen Liu, Bingling Li
Summary: The development of an accurate and practical electrochemical biosensor for the detection of kanamycin residues in water samples is described. The sensor demonstrates a simple construction method, good selectivity, and stability, and can provide rapid or real-time detection of kanamycin residues in water samples.
CHINESE JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuetong Yang, Derong Kong, Yungen Wu, Yiheng Chen, Changhao Dai, Chang Chen, Junhong Zhao, Shi Luo, Wentao Liu, Yunqi Liu, Dacheng Wei
Summary: A graphene field-effect transistor (GFET) biosensor based on a tetrahedral DNA nanostructure (TDN)-assisted catalytic hairpin assembly (CHA) reaction (TCHA) has been developed, allowing sensitive and specific detection of miRNA-21. The TDN structures in the biosensing interface facilitate the CHA reaction and prevent unwanted entanglements, aggregation, and adsorption of probes on the graphene channel. The biosensor exhibits ultra-sensitive detection of miRNA-21, with a detection limit of 5.67 x 10(-19) M, three orders of magnitude lower than that achieved by graphene transistors with single-stranded DNA probe functionalization of the channel. Furthermore, the biosensor demonstrates excellent performance in terms of selectivity, stability, and reproducibility, showing promising potential in bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yuetong Yang, Derong Kong, Yungen Wu, Yiheng Chen, Changhao Dai, Chang Chen, Junhong Zhao, Shi Luo, Wentao Liu, Yunqi Liu, Dacheng Wei
Summary: Graphene field-effect transistors (GFETs) offer simple and label-free diagnostics for sensitive and specific detection of microRNAs (miRNAs). Researchers have developed a GFET biosensor based on tetrahedral DNA nanostructures (TDN) that facilitates catalytic hairpin assembly (CHA) reaction for ultrasensitive detection of miRNA-21. The biosensor exhibits excellent analytical performance and has been successfully applied in complex serum environments and cell lysates, showing promising potential in bioanalysis and clinical diagnosis.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Shuju Zhao, Zhen Song, Tao Liu, Xin Wang, Yanxin Li, Yaqun Xu, Hao Wang, Yumin Wu, Xiliang Luo
Summary: Zwitterionic peptides with strong water binding ability have been designed to construct antifouling biosensors with enhanced stability in complex biological fluids. The designed peptides demonstrated much stronger antifouling capability and significantly enhanced stability in human serum and blood. Furthermore, the biosensor integrating the designed antifouling peptide with CEA aptamer exhibited a great sensitivity for detecting CEA protein, making it promising for practical applications.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biophysics
Skomantas Serapinas, Justina Gineityte, Marius Butkevicius, Rapolas Danilevicius, Marius Dagys, Dalius Ratautas
Summary: This paper presents a new bioelectronic device for the detection of nuclease activity in various fluids. The developed sensor enables rapid and sensitive determination of DNase activity, with high accuracy and reproducibility demonstrated in real human saliva samples. The technology has the potential to improve nuclease quality control processes in the pharmaceutical/biotechnology industry and provide new insights into the importance of nucleases for medical applications.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Electrochemistry
Yan Wu, Ning Li, Shasha Xu, Xiaoyu Chong, Changgeng Zhang
Summary: The study focuses on the immobilization of Hepatitis B virus-specific DNA probes on gold electrodes using a self-assembled single-molecule membrane method. Results showed that probe concentration and immobilization time significantly affect the immobilization efficiency. Additionally, a DNA electrochemical sensor was successfully used for the qualitative and quantitative detection of complementary DNA in solution and Hepatitis B virus detection in serum.
INTERNATIONAL JOURNAL OF ELECTROCHEMICAL SCIENCE
(2022)
Article
Chemistry, Multidisciplinary
Sakshi Pareek, Vishwadeep Rout, Utkarsh Jain, Mausumi Bharadwaj, Nidhi Chauhan
Summary: A new DNA-based electrochemical genosensor was developed for efficient detection of HPV-18 in cervical cancer. The sensor showed high selectivity and sensitivity, making it a rapid screening tool for HPV-18.
Article
Chemistry, Analytical
Omair Adil, Seth B. Eddington, Keith T. Gagnon, Mohtashim H. Shamsi
Summary: Overgrowth of short tandem repeat sequences in genes can cause neurodegenerative disorders. These repeat sequences are ideal targets for label-free electrochemical detection, but their secondary structures and sporadic repeats can complicate detection. By analyzing different nucleic acid microprobe backbones, the study found that the lock-nucleic acid (LNA) microprobe can specifically detect tandem repeats and perform better with the presence of Mg2+. The LNA-based platform shows potential for the development of a reliable biosensor for genetic neurodegenerative disorders.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Nan Ma, Jingliang Liu, Shuaibing Yu, Shiyu Liu, Lianzhi Li, Jinming Kong, Xueji Zhang
Summary: This study reports for the first time the relationship between R-Co(III) free radical persistent free radical effect and vitamin B-12 circulation and the perception of the ultralow abundance of microRNA-21, a lung cancer biomarker.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Analytical
Esmaeel Alipour, Sheida Norouzi, Shokoufeh Moradi
Summary: An electrochemical DNA biosensor using quercetin as an electroactive indicator was developed for specific target DNA detection, achieving a detection limit of 83 pM with a relative standard deviation of 4.6%. The biosensor was successfully applied for the detection of short sequences of hepatitis C virus and showed selective response to the target DNA.
ANALYTICAL METHODS
(2021)
Article
Chemistry, Physical
Yang Sun, Shicai Xu, Tiying Zhu, Jiajun Lu, Shuo Chen, Maomao Liu, Guangcan Wang, Baoyuan Man, Huamin Li, Cheng Yang
Summary: In this study, a photocurrent-modulated FET sensor for DNA hybridization detection was proposed, achieving a detection limit down to attomolar concentrations. The sensor significantly enhances the response signal compared to traditional FET sensors, opening new avenues for further development in biosensing applications.
Article
Biophysics
Hanjun Chen, Ying Liu, Shaoqiong Feng, Yu Cao, Tingting Wu, Zhihong Liu
Summary: A cotton thread-based multi-channel photothermal biosensor was developed for simultaneous detection of multiple breast cancer-related miRNAs. The biosensor exhibited excellent specificity and sensitivity, with detection limits of 37 pM for miRNA-10b, 38 pM for miRNA-27a, and 38 pM for miRNA-let-7a. The biosensor also showed great potential in clinical diagnosis.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Chemistry, Multidisciplinary
Siti Fatimah Abd Rahman, Nor Azah Yusof, Mohd Khairuddin Md Arshad, Uda Hashim, Mohammad Nuzaihan Md Nor, Mohd Nizar Hamidon
Summary: A highly sensitive silicon nanowire-based sensor device was developed using electron beam lithography integrated with CMOS technology. The device showed good alignment and linearity for pH sensing, and could also be used for detecting specific ssDNA molecules.
Article
Chemistry, Analytical
Hui Xi, Hanlin Jiang, Mario Juhas, Yang Zhang
Summary: Noncanonical G-quadruplex nucleic acid structures have been used as probes in biosensors for accurate and efficient detection of metal ions, proteins, and nucleic acids. In this study, a reliable and efficient fluorescent biosensor platform for G-quadruplex based detection of the human AGT protein was constructed using the magnetic bead enrichment method. This biosensor provides high accuracy, speed, and low cost, and successfully detected AGT at the cellular level.
Article
Chemistry, Analytical
Zheng Wei Wong, Siu Yee New
Summary: A fluorescence biosensor based on hybridisation chain reaction amplification coupled with silver nanoclusters has been developed for nucleic acid detection, providing a reliable and low risk method for detecting target DNA.
Article
Cell Biology
Bingling Dai, Tianfeng Yang, Yujiao Ma, Nan Ma, Xianpeng Shi, Dongdong Zhang, Jie Zhang, Yanmin Zhang
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2018)
Article
Biochemical Research Methods
Man Zhu, Yuxin Cui, Liu Yang, Tianfeng Yang, Hongying Wang, Dongdong Zhang, Weina Ma, Yanmin Zhang
JOURNAL OF CHROMATOGRAPHY A
(2019)
Article
Chemistry, Analytical
Xia Chen, Dandan Li, Weina Ma, Tianfeng Yang, Yanmin Zhang, Dongdong Zhang
Article
Cell Biology
Man Zhu, Liu Yang, Xianpeng Shi, Zhengyan Gong, Runze Yu, Dongdong Zhang, Yanmin Zhang, Weina Ma
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Materials Science, Biomaterials
Dongdong Zhang, Xia Chen, Weina Ma, Tianfeng Yang, Dandan Li, Bingling Dai, Yanmin Zhang
MATERIALS SCIENCE AND ENGINEERING C-MATERIALS FOR BIOLOGICAL APPLICATIONS
(2019)
Article
Cell Biology
Qi Su, Jingjing Wang, Mengying Fan, Mohsin Ahmad Ghauri, Asmat Ullah, Bo Wang, Bingling Dai, Yingzhuan Zhan, Dongdong Zhang, Yanmin Zhang
JOURNAL OF CELLULAR AND MOLECULAR MEDICINE
(2020)
Article
Chemistry, Analytical
Dandan Li, Mei Liu, Yingzhuan Zhan, Qi Su, Yanmin Zhang, Dongdong Zhang
Article
Pharmacology & Pharmacy
Man Zhu, Xianpeng Shi, Zhengyan Gong, Qi Su, Runze Yu, Bo Wang, Tianfeng Yang, Bingling Dai, Yingzhuan Zhan, Dongdong Zhang, Yanmin Zhang
PHARMACOLOGICAL RESEARCH
(2020)
Article
Chemistry, Physical
Lihua Shen, Jianhang Wei, Zhifang Liu, Zhuangwei Bai, Yuangang Li, Dongdong Zhang, Chengxiao Zhang
CHEMISTRY OF MATERIALS
(2020)
Article
Oncology
Tianfeng Yang, Rui Xu, Jian Huo, Bo Wang, Xia Du, Bingling Dai, Man Zhu, Yingzhuan Zhan, Dongdong Zhang, Yanmin Zhang
Summary: This study reveals that toosendanin (TSN) may be a novel druggable WWOX agonist for metastatic hepatocellular carcinoma (HCC) patients, showing significant anti-proliferative and anti-metastatic effects on HCC cells in a WWOX-dependent manner. TSN regulates multiple signaling pathways by altering their interactions with WWOX, providing potential therapeutic implications for HCC treatment.
Article
Plant Sciences
Mohsin Ahmad Ghauri, Qi Su, Asmat Ullah, Jingjing Wang, Ammar Sarwar, Qing Wu, Dongdong Zhang, Yanmin Zhang
Summary: SAN has been found to inhibit cell proliferation, migration, and colonization characteristics in breast cancer, as well as showing significant inhibitory effects on EMT regulating protein expression. In animal model, SAN exhibits prominent anti-metastatic and anti-proliferative effects.
Article
Pharmacology & Pharmacy
Junqiang Pan, Mei Liu, Dandan Li, Haonan Zheng, Dongdong Zhang
Summary: A novel ternary nanocomposite with excellent electro-catalytic activity was successfully prepared for simultaneous detection of dopamine and uric acid under physiological conditions.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Electrochemistry
Jiatao Chen, Xian Ran, Xiuting Ding, Dongdong Zhang
Summary: In this study, a simple, rapid, and stable electrochemical flow injection amperometry (FI-Amp) system was constructed to investigate the redox mechanism of dopamine (DA). The system utilized a flow cell with two glassy carbon electrodes arranged in series. Dual quantitative analysis of DA was achieved by the oxidation current of DA on the first working electrode (WE1) and the reduction current of its oxidation product on the second working electrode (WE2). The FI-Amp system exhibited excellent repeatability and stability, making it a promising tool for studying molecular redox reactions and electrochemical identification of other electroactive molecules.
ELECTROCHEMISTRY COMMUNICATIONS
(2023)
Article
Chemistry, Analytical
Dongdong Zhang, Jiatao Chen, Xia Chen, Xiuting Ding, Yanmin Zhang
Summary: A new insight for the non-oxidative electrochemical analysis of dopamine (DA) was proposed through reverse voltammetry preoxidation and intramolecular cyclization. This method allows for dual quantitative analysis of DA in the presence of high concentrations of interfering substances.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2023)
Article
Nanoscience & Nanotechnology
Zhuangwei Bai, Lihua Shen, Jianhang Wei, Yuangang Li, Azhar Abbas, Yinyan Li, Mengnan Qu, Dongdong Zhang, Chengxiao Zhang
ACS APPLIED NANO MATERIALS
(2020)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)