Article
Chemistry, Analytical
Bocheng Tu, Zishan Feng, Haitao Wang, Wei Zhang, Weicong Ye, Hongbo Wang, Xianjin Xiao, Wenbo Zhao, Tongbo Wu
Summary: This new method utilizes lambda exonuclease and a background signal suppression probe, which successfully suppresses the background signal through probe structure design to achieve highly sensitive OGG detection. The detection limit is 5.0 x 10(-4) U mL(-1), and its potential clinical application value was demonstrated in biological samples.
ANALYTICA CHIMICA ACTA
(2021)
Article
Biotechnology & Applied Microbiology
Imen Tanniche, Amanda K. Fisher, Frank Gillam, Eva Collakova, Chenming Zhang, David R. Bevan, Ryan S. Senger
Summary: Lambda-PCR is an effective method for DNA assembly and cloning, which utilizes overlap extension and different variants to achieve stable, efficient, and cost-effective assembly of DNA sequences.
BIOTECHNOLOGY AND BIOENGINEERING
(2022)
Article
Chemistry, Analytical
Zhou-Jie Liu, Liang-Yong Yang, Tai-Cheng Lu, Yu-Qi Liang, Meng-Meng Liu, Guang-Xian Zhong, Xin-Hua Lin, Pin-Fang Huang, Jin-Yuan Chen
Summary: This study presents a lambda exonuclease-assisted ligase chain reaction-based electrochemical biosensor, named lambda-eLCR, for highly sensitive and accurate genotyping of the CYP2C19*2 allele. The biosensor features a methylene blue-attached DNA probe and an inverted hybridization format, enabling zero-background analysis. The lambda-eLCR exhibited excellent performance in terms of a good linear range and low limit of detection, and successfully distinguished the CYP2C19*2 allele genotypes in human whole blood samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Multidisciplinary
Zhaohui Qin, Yu Liu, Linghao Zhang, Jiajia Liu, Xin Su
Summary: This study demonstrates a DNA-based artificial dissipative system with a molecular timer, which allows for dynamic enzyme catalysis and DNA nanostructure disassembly. This provides a useful tool for the development of artificial reaction networks and intelligent nanostructures.
Article
Chemistry, Analytical
Zhen Liu, Chen Liu, Liqiong He, Jinquan Liu, Le Li, Shengyuan Yang, Yan Tan, Xing Liu, Xilin Xiao
Summary: An ultrasensitive DNAzyme-based fluorescence biosensor for detecting Cu2+ was designed using cascade signal amplification strategy. The detection system utilized the activation of Cu2+-dependent DNAzyme by Cu2+ and subsequent reactions to generate a strong fluorescent signal. The biosensor showed excellent detection performance and potential applications.
Article
Chemistry, Analytical
Tingting Hu, Changbei Ma, Ying Yan, Junxiang Chen
Summary: A sensitive method for detecting DNA methyltransferase (MTase) activity was developed using fluorescence resonance energy transfer (FRET) and exonuclease (Exo) III-mediated signal amplification. This method showed high sensitivity with a lowest detection limit of 0.045 U/mL for MTase, and was also suitable for MTase-inhibitor screening.
Article
Chemistry, Analytical
Hong Ran Tao, Hang Yan, Zhe Sun, Ya Bin Cui, Nian Bing Li, Hong Qun Luo
Summary: In this study, a ratiometric fluorescence DNA nanozipper was developed for highly sensitive detection of ATP. The sensing strategy showed a linear response to ATP concentration and could detect low concentrations, demonstrating promising application potential in biological samples.
SENSORS AND ACTUATORS B-CHEMICAL
(2024)
Article
Biochemical Research Methods
Huikai Ma, Ping Wang, Yaoyao Xie, Jinghan Liu, Wei Feng, Sanqiang Li
Summary: A novel ratiometric electrochemical aptasensor was developed for the detection of CEA, showing great potential in diluted serum detection. The aptasensor utilized a hairpin DNA probe and exonuclease I-assisted target recycling amplification strategy to achieve the detection of CEA through the change in ratiometric electrochemical signal.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Jianbo Sun, Changjiang Li, Yuqiang Hu, Yawen Ding, Tongbo Wu
Summary: A rapid, sensitive, and selective biosensor for UDG activity detection has been developed in this study based on the substrate preference of Lambda exonuclease. The proposed strategy exhibited high selectivity and sensitivity, and has been successfully applied to detect UDG in real biological samples and screen UDG inhibitors.
Article
Chemistry, Analytical
Wenqian Yuan, Zhen Zhang, Yuqiang Hu, Minghao Hu, Zhe Hu, Tongbo Wu
Summary: Endonuclease IV (Endo IV) is an important enzyme in biochemical research due to its ability to hydrolyze the apurinic/apyrimidinic site (AP site) in double-strand DNA (dsDNA). This study investigated the hydrolysis activity of Endo IV towards a dsDNA substrate with an atypical structure. The results showed that a single nucleotide change around the AP site could significantly affect the cleavage activity of Endo IV. Based on this finding, a sensitive and selective single nucleotide variation (SNV) detection strategy was proposed, which achieved a detection limit of 0.05% mutation abundance for clinical-related SNVs.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Multidisciplinary Sciences
Jungmin Yoo, Donghun Lee, Hyeryeon Im, Sangmi Ji, Sanghoon Oh, Minsang Shin, Daeho Park, Gwangrog Lee
Summary: The study reveals that during base excision repair, Exonuclease III creates a gap and transient ssDNA loop through an AP site anchor-based mechanism. The size of the gap is determined by the rigidity of the ssDNA loop and the duplex stability of the DNA.
Article
Spectroscopy
Boxu Wang, Zheng Liu, Zhihong Li, Ningyi Xu, Xuejiao Zhang, Ruifang Su, Junyang Wang, Rui Jin, Chunyan Sun
Summary: In this study, a fluorescent probe was constructed for facile and sensitive detection of Hg2+ based on the unique fluorescent property of 2-aminopurine (2-AP). The probe exhibited an excellent linear relationship with Hg2+ concentration and had a low detection limit. Satisfactory detection results were obtained in water and fish samples.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Qirong Chen, Huahui Gao, Jianglong Yao, Bingying Jiang, Ruo Yuan, Yun Xiang
Summary: In this study, a new target/aptamer binding-induced inhibition of enzyme activity strategy was used to construct an amplified and sensitive electrochemical biosensor for detecting Sonic Hedgehog (SHh). The sensor exhibited high selectivity and could detect SHh down to 3.34 pM. This strategy has the potential to be a convenient approach for detecting different proteins in biological studies and disease diagnosis.
SENSORS AND ACTUATORS B-CHEMICAL
(2023)
Article
Biochemistry & Molecular Biology
Stefan H. Mueller, Lucy J. Fitschen, Afnan Shirbini, Samir M. Hamdan, Lisanne M. Spenkelink, Antoine M. van Oijen
Summary: Traditional enzyme characterization methods only provide population averages, while single-molecule methods can reveal kinetics and population heterogeneity but are often complex and time-consuming. We present a high-throughput single-molecule assay for rapidly characterizing DNA metabolism-related proteins by measuring changes in fluorescence intensity. Our method, combined with automated data analysis, can provide enzymatic activity data for thousands of molecules in less than an hour. We demonstrated three different enzyme activities and discovered a previously unknown activity of the UvrD helicase.
NUCLEIC ACIDS RESEARCH
(2023)
Article
Chemistry, Analytical
Haining Cui, Keqi An, Chengquan Wang, Yangfang Chen, Suli Jia, Jing Qian, Nan Hao, Jie Wei, Kun Wang
Summary: A disposable ratiometric electrochemical aptasensor was constructed for highly sensitive detection of aflatoxin B1. Au nanoflowers on ITO surface were used for DNA anchoring and methylene blue labeling. The detection was based on the peak current intensity ratio (I-MB/I-Fc) as the signal transducer. The aptasensor exhibited a wide detection range and a low detection limit.
SENSORS AND ACTUATORS B-CHEMICAL
(2022)
Article
Chemistry, Analytical
Jimmy Ming-Yuan Huang, Grace Henihan, Daniel Macdonald, Annette Michalowski, Kate Templeton, Alan P. Gibb, Holger Schulze, Till T. Bachmann
ANALYTICAL CHEMISTRY
(2015)
Article
Biophysics
Grace Henihan, Holger Schulze, Damion K. Corrigan, Gerard Giraud, Jonathan G. Terry, Alison Hardie, Colin J. Campbell, Anthony J. Walton, Jason Crain, Ronald Pethig, Kate E. Templeton, Andrew R. Mount, Till T. Bachmann
BIOSENSORS & BIOELECTRONICS
(2016)
Article
Microbiology
Dalal Saleh Al-Rubaye, Grace Henihan, Ameraa K. Abas Al-Abasly, Amie-Louise Seagar, Azhar Ab. F. Al-Attraqchi, Holger Schulze, Dhafer Salman Hashim, Jinan Khalid Kamil, Ian F. Laurenson, Till T. Bachmann
JOURNAL OF MEDICAL MICROBIOLOGY
(2016)
Article
Chemistry, Analytical
D. K. Corrigan, H. Schulze, I. Ciani, G. Henihan, A. R. Mount, T. T. Bachmann
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Damion K. Corrigan, Vincent Vezza, Holger Schulze, Till T. Bachmann, Andrew R. Mount, Anthony J. Walton, Jonathan G. Terry
Article
Chemistry, Multidisciplinary
Alfredo E. Ongaro, Ieva Keraite, Antonio Liga, Gioacchino Conoscenti, Stuart Coles, Holger Schulze, Till T. Bachmann, Khaled Parvez, Cinzia Casiraghi, Nicola Howarth, Vincenzo La Carubba, Maiwenn Kersaudy-Kerhoas
ACS SUSTAINABLE CHEMISTRY & ENGINEERING
(2018)
Article
Chemistry, Analytical
D. K. Corrigan, H. Schulze, G. Henihan, A. Hardie, I. Ciani, G. Giraud, J. G. Terry, A. J. Walton, R. Pethig, P. Ghazal, J. Crain, C. J. Campbell, K. E. Templeton, A. R. Mount, T. T. Bachmann
Article
Chemistry, Analytical
Yoshihiko Nonaka, Wataru Yoshida, Koichi Abe, Stefano Ferri, Holger Schulze, Till T. Bachmann, Kazunori Ikebukuro
ANALYTICAL CHEMISTRY
(2013)
Article
Biophysics
Ilenia Ciani, Holger Schulze, Damion K. Corrigan, Grace Henihan, Gerard Giraud, Jonathan G. Terry, Anthony J. Walton, Ronald Pethig, Peter Ghazal, Jason Crain, Colin J. Campbell, Till T. Bachmann, Andrew R. Mount
BIOSENSORS & BIOELECTRONICS
(2012)
Article
Chemistry, Analytical
Damion K. Corrigan, Holger Schulze, Rachel A. McDermott, Ilka Schmueser, Grace Henihan, John B. Henry, Till T. Bachmann, Andrew R. Mount
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2014)
Article
Chemistry, Multidisciplinary
Shahida N. Syed, Holger Schulze, Daniel Macdonald, Jason Crain, Andrew R. Mount, Till T. Bachmann
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2013)
Review
Chemistry, Analytical
Gerard Cummins, Jessica Kremer, Anne Bernassau, Andrew Brown, Helen L. Bridle, Holger Schulze, Till T. Bachmann, Michael Crichton, Fiona C. Denison, Marc P. Y. Desmulliez
Article
Chemistry, Analytical
Holger Schulze, Harry Wilson, Ines Cara, Steven Carter, Edward N. Dyson, Ravikrishnan Elangovan, Stephen Rimmer, Till T. Bachmann
Summary: The study has developed a new rapid bacterial infection diagnosis method that offers simpler test complexity and higher discrimination compared to existing detection methods.
Article
Chemistry, Analytical
Holger Schulze, Andrew Arnott, Adriana Libori, Eleojo A. Obaje, Till T. Bachmann
Summary: Antibiotic resistance, especially in Gram-negative bacteria, is a major concern. The development of rapid molecular diagnostic tests for detecting colistin resistance is crucial in combating the rise of antimicrobial resistance.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemical Research Methods
Maiwenn Kersaudy-Kerhoas, Antonio Liga, Appan Roychoudhury, Marilena Stamouli, Rhiannon Grant, Damaso Sanchez Carrera, Holger Schulze, Witold Mielczarek, Wilna Oosthuyzen, Juan F. Quintana, Paul Dickinson, Amy H. Buck, Nicholas R. Leslie, Jurgen Haas, Till T. Bachmann, James W. Dear
Summary: Drug-induced liver injury (DILI) is a major cause of hospital attendance and financial loss, and miR-122 has been suggested as a potential marker for DILI. However, the measurement of circulating miRNAs at point of care is time-consuming and expensive. This study presents a novel microfluidic platform for the extraction of miR-122 from blood, which demonstrates comparable or better performance than standard extraction kits. The platform, combined with electrochemical impedance spectroscopy, shows potential for point-of-care detection of miR-122, providing a standardized and low-cost method for miRNA-based assays.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)