Article
Biochemistry & Molecular Biology
Shuhei Nishizawa, Gaohong Tu, Daisuke Ogata, Kouichiro Miyauchi, Akihiro Ohkubo
Summary: In this study, modified bases that can recognize T-A base pairs were developed by introducing quinoline derivatives into antiparallel TFOs. The ability of these modified bases to form triplexes and discriminate between base pairs was evaluated.
BIOORGANIC & MEDICINAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Dongqi Ni, Kang Huang, Hui Wang, Weixian Zhou, Mengyu Guo, Didar Baimanov, Yueguang Xue, Yandong Chen, Ying Liu
Summary: Antigene technology based on triplex-forming oligonucleotide (TFO) is widely used in gene-specific regulation as a potential gene therapy method. Optimizing fluorine atom (F) substitution of TFO can greatly improve the stability of triplex DNA. By combining nucleotide analogue floxuridine, a two-in-one chemo-TFO is developed, which has both gene regulation and chemotherapeutic functions.
Review
Pharmacology & Pharmacy
Yu Mikame, Asako Yamayoshi
Summary: Recent developments in artificial nucleic acid and drug delivery systems have opened up new avenues for the treatment of therapeutic oligonucleotides, offering conceptual advantages for symbiotic genome-targeting tools and complete cure of diseases. However, the potential off-target, cytotoxic, and/or immunogenic effects of nuclease-based gene-editing tools may hinder their in vivo applications.
Article
Biotechnology & Applied Microbiology
Ling Zhi Wu, Yuan Ye, Zhi Xuan Wang, Die Ma, Li Li, Guo Hao Xi, Bi Qing Bao, Li Xing Weng
Summary: A novel SNP detection assay has been developed for single-nucleotide discrimination using a nanopore sensing platform with DNA probed Au nanoparticles as transport carriers. The study successfully detected the SNP of p53 gene mutation in gastric cancer at femtomolar concentration, offering high sensitivity and spatial resolution for potential applications in early diagnosis and disease prevention.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Chemistry, Multidisciplinary
Cindy Ng, Anirban Samanta, Ole Aalund Mandrup, Emily Tsang, Sarah Youssef, Lasse Hyldgaard Klausen, Mingdong Dong, Minke A. D. Nijenhuis, Kurt V. Gothelf
Summary: Engineered architectural control over double-stranded DNA (dsDNA) is challenging, but the triplex origami methodology provides a solution. Triplex-forming oligonucleotides (TFOs) are used to bind purines in dsDNA and compact it into well-defined objects with various structural features. The methodology allows for modulating the length of integrated and free-standing dsDNA loops with high efficiency and enables unprecedented spatial control over dsDNA templates.
ADVANCED MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Hongyu Wu, Chengwen Zhang, Fulin Zhu, Yu Zhu, Xinhui Lu, Ying Wan, Shao Su, Jie Chao, Lianhui Wang, Dan Zhu
Summary: Here, a poly-adenine (polyA)-mediated programmable FRET-nanoflare is presented for ratiometric intracellular ATP imaging with anti-interference capability. The programmable polyA attachment enhances the signal response for ATP, and the FRET-based nanoflare avoids false-positive signals due to probe degradation in complex environments, showing great potential for clinical diagnosis.
CHEMICAL COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Nikita Brodyagin, Ilze Kumpina, Justin Applegate, Martins Katkevics, Eriks Rozners
Summary: The research reveals that a new type of peptide nucleic acid can form stable triple helix structures with double-stranded RNA, improving the recognition of dsRNA. The study suggests insights for future development of new nucleobases to expand the sequence scope of noncoding dsRNAs that can be targeted by triplex-forming PNAs.
ACS CHEMICAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Brionna McGorman, Nicolo Zuin Fantoni, Sinead O'Carroll, Anna Ziemele, Afaf H. El-Sagheer, Tom Brown, Andrew Kellett
Summary: In this study, a enzymatic protocol was developed for the preparation of chemical nuclease-TFO hybrid constructs, which includes the incorporation of novel artificial metallo-nuclease (AMN)-dNTPs into the TFO strand. The specificity of enzymatically synthesised DPA-TFOs was improved through coordinated designer intercalators, and high-precision DNA cleavage was achieved. This research demonstrates the potential of chemical nuclease-TFOs in overcoming the limitations of non-molecularly targeted metallodrugs and advancing artificial gene-editing technology.
NUCLEIC ACIDS RESEARCH
(2022)
Article
Chemistry, Medicinal
Yu Mikame, Honoka Eshima, Haruki Toyama, Juki Nakao, Misaki Matsuo, Tsuyoshi Yamamoto, Yoshiyuki Hari, Jun A. Komano, Asako Yamayoshi
Summary: In this study, we investigated the photo-crosslinking properties of psoralen-conjugated triplex-forming oligonucleotides (Ps-TFOs) using novel Ps-TFOs containing 2'-O,4'-C-methylene-bridged nucleic acids (Ps-LNA-mixmer). We found that the photo-crosslinking process of Ps-TFOs was not sufficient under cell irradiation conditions and highlighted the need to develop more reactive psoralen derivatives.
Article
Chemistry, Multidisciplinary
Emma E. Coughlin, Jingtian Hu, Andrew Lee, Teri W. Odom
Summary: This paper demonstrates the light-directed functionalization of anisotropic gold nanoparticles with different thiolated-DNA oligomer sequences, enabling the creation of nanoparticle superstructures with precise control over oligo presentation at the single-particle level.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2021)
Article
Chemistry, Multidisciplinary
Joseph Hennessy, Brionna McGorman, Zara Molphy, Nicholas P. Farrell, Daniel Singleton, Tom Brown, Andrew Kellett
Summary: A click chemistry approach was used to generate a library of Pt-II-TFO hybrids, which were able to enhance target binding and discrimination between target and off-target sequences by incorporating modifications of thiazole orange. This method offers a potential solution to the limitations of clinical platinum(II) therapeutics.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2022)
Article
Chemistry, Multidisciplinary
Christopher A. Ryan, Md Motiar Rahman, Vipin Kumar, Eriks Rozners
Summary: RNA folding is influenced by the presence of bulges consisting of unpaired nucleotides, which play a significant role in stabilizing various interactions involving RNA. This study demonstrates that triplex-forming peptide nucleic acids (PNAs) have a high affinity for single-purine-nucleotide bulges in double-helical RNA. The formation of the RNA-PNA triplex alters the equilibrium between looped out and stacked-in conformations, potentially providing a valuable tool for studying RNA structure-function relationships and developing therapeutic strategies targeting disease-related RNAs.
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2023)
Article
Chemistry, Multidisciplinary
Feng Chen, Qiujun Lu, Linna Huang, Biwu Liu, Meiling Liu, Youyu Zhang, Juewen Liu
Summary: The research team designed DNA nanosensors to monitor K+ and pH in lysosomes, and co-imaging revealed that the influx of H+ was accompanied by the efflux of K+, solving a long-standing issue related to lysosomal acidification.
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2021)
Article
Chemistry, Physical
Maria Enea, Miguel Peixoto de Almeida, Andre Dias, Beatriz Ferreira, Carlos Bernardes, Orfeu Flores, Eulalia Pereira, Ricardo Franco
Summary: Gold nanoparticles (AuNPs) have unique plasmonic properties and are widely used in biomedical diagnostics. In this study, the properties of 20 nm AuNPs and 35 nm AuNPs were compared when used in a colorimetric assay for the detection of a single nucleotide polymorphism related to lactose intolerance. The results showed that both sizes of AuNPs had similar detection limits, but the use of 35 nm AuNPs allowed for significant reductions in the amount of gold and oligonucleotide used in the assay.
PARTICLE & PARTICLE SYSTEMS CHARACTERIZATION
(2022)
Article
Chemistry, Multidisciplinary
Yu Zhang, Mengke Su, Xingru Fang, Wenwen Huang, Hao Jiang, Qi Li, Nisar Hussain, Mao Ye, Honglin Liu, Weihong Tan
Summary: In this study, a novel nanometal surface energy transfer (NSET) nanoruler with a single-nucleobase resolution was proposed for in situ identification of aptamer-binding targets on living cell membrane surfaces. The nanoruler consisted of FAM-labeled aptamers and single-sized gold nanoparticle (GNP) antibody conjugates as a donor and an acceptor, respectively. Experimental evidence demonstrated the single nucleobase resolution of the nanoruler by molecular size, orientation, quenching nature, and other dye-GNP pairs. The nanoruler showed high reproducibility and precision for measuring molecule distance on living cell membranes at the nanometer level due to the use of only single-sized antibody-capped GNPs. The proposed strategy represents a solid step towards the wider application of optical-based rulers for observing molecular structural configuration and dynamic transitions on the membrane surface of living cells.
Article
Chemistry, Physical
Xiaoli Zhu, Xiaoxia Mao, Zihan Wang, Chang Feng, Guifang Chen, Genxi Li
Article
Chemistry, Analytical
Xiaonan Wang, Ying Liu, Juan Zhang, Genxi Li
SENSORS AND ACTUATORS B-CHEMICAL
(2017)
Article
Medicine, Research & Experimental
Chang Feng, Bing Bo, Xiaoxia Mao, Hai Shi, Xiaoli Zhu, Genxi Li
Article
Chemistry, Physical
Jin Jia, Weijia Zhou, Zhaoqian Wei, Tanli Xiong, Guixiang Li, Lili Zhao, Xiaofei Zhang, Hong Liu, Jian Zhou, Shaowei Chen
Article
Medicine, Research & Experimental
Xiaoxia Chen, Jing Zhao, Tianshu Chen, Tao Gao, Xiaoli Zhu, Genxi Li
Article
Multidisciplinary Sciences
Chao Li, Liu Shi, Yaqin Tao, Xiaoxia Mao, Yang Xiang, Genxi Li
SCIENTIFIC REPORTS
(2017)
Article
Chemistry, Analytical
Yuxin Lin, Beibei Nie, Xinyu Qu, Minghui Wang, Jie Yang, Genxi Li
Summary: Covalent organic frameworks (COFs) have attracted much attention for their unique physical-chemical characteristics and potential applications in biosensing. In this study, spherical COFs with regular shape and good dispersion were synthesized, and a novel nanoprobe was developed by modifying Histostar on the COFs surface. The nanoprobe was then applied to fabricate an electrochemical biosensor for exosomes detection, achieving a low detection limit and wide linear detection range. This work offers a promising platform for ultrasensitive detection of exosomes.
Article
Chemistry, Analytical
Cao Ya, Kang Ming-Yang, Chen Hong, Tang Ying-Ying, Feng Chang, Zhao Jing, Li Gen-Xi
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Juan Zhang, Xiaonan Wang, Tingjun Chen, Chang Feng, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Chao Li, Yaqin Tao, Yi Yang, Chang Feng, Yang Xiang, Genxi Li
Article
Chemistry, Analytical
Tao Gao, Bei Wang, Liu Shi, Xiaoli Zhu, Yang Xiang, Jun-ichi Anza, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Analytical
Chao Li, Yaqin Tao, Yi Yang, Yang Xiang, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Chemistry, Multidisciplinary
Xiaolu Hu, Chao Li, Chang Feng, Xiaoxia Mao, Yang Xiang, Genxi Li
CHEMICAL COMMUNICATIONS
(2017)
Article
Chemistry, Analytical
Xiaoli Zhu, Yaoyao Chen, Chang Feng, Wei Wang, Bing Bo, Ruixin Ren, Genxi Li
ANALYTICAL CHEMISTRY
(2017)
Article
Biophysics
Jinlong Li, Tao Gao, Shiyu Gu, Jun Zhi, Jie Yang, Genxi Li
BIOSENSORS & BIOELECTRONICS
(2017)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)