Article
Biophysics
Yuxia Fan, Weizhu Chen, Ninglong Zhang, Mingyang Li, Yiwen Zhu, Gaole Chen, Yin Zhang, Yuan Liu
Summary: A self-assembled biosensor based on matured taste receptor cells (TRCs) was developed, which successfully detected L-monosodium glutamate (MSG) and other umami substances, providing insights into taste signal transduction mechanisms and suggesting the potential for biomimetic sensors in intelligent perception applications.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Biophysics
Yuxia Fan, Yulin Huang, Ninglong Zhang, Gaole Chen, Shui Jiang, Yin Zhang, Guangchang Pang, Wenli Wang, Yuan Liu
Summary: This study investigated the distribution and signal coding logic of umami receptors on the tongue using taste bud biosensors. The results showed different response currents of taste bud biosensors to MSG and IMP, with T1R1 receptor being more expressed in the rostral tongue cells with higher sensitivity to MSG. This method has the potential to explain the interaction mechanism of umami substances with their receptors more accurately and develop artificial intelligent taste sensory.
BIOSENSORS & BIOELECTRONICS
(2022)
Article
Pharmacology & Pharmacy
Yanxuan Xiao, Hangfan Zhou, Lu Jiang, Rui Liu, Qianming Chen
Summary: Taste is mediated by specialized neuroepithelial cells called taste receptor cells located at tongue papillae. Ion channels play a crucial role in various cell physiological activities, while epigenetic modifications regulate gene expression without altering DNA sequence.
PHARMACOLOGICAL RESEARCH
(2021)
Review
Ecology
Daniel Berning, Joshua B. Gross
Summary: Animals in subterranean environments evolve regressive and constructive features. While regressive traits like vision and pigmentation are reduced, constructive traits like non-visual sensation are commonly enhanced. The gustatory system, an important constructive sensory feature in cave animals, remains poorly understood. This review highlights the differences in gustation between cave and surface fish, identifies knowledge gaps in constructive taste system evolution, and emphasizes the need for further research on the genetic and developmental mechanisms underlying constructive traits.
FRONTIERS IN ECOLOGY AND EVOLUTION
(2023)
Article
Biophysics
Jiansen Li, Wenli Wang, Jing Liu, He Li, Ninglong Zhang, Fengzhen Yang, Haowei Dong, Xia Sun, Gaole Chen, Yuxia Fan, Yemin Guo, Yuan Liu
Summary: In this study, an electrochemical biosensor based on a double-signal amplification strategy was constructed using single-walled carbon nanotubes (SWCNTs) and Prussian blue (PB), to successfully quantify the umami taste of four representative substances. The biosensor showed high sensitivity and selectivity in detecting umami substances with a normalized signal intensity of over 90%.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Shuge Liu, Ping Zhu, Yulan Tian, Yating Chen, Yage Liu, Miaomiao Wang, Wei Chen, Liping Du, Chunsheng Wu
Summary: A taste bud organoid-based biosensor was developed for the study of taste sensations. Results showed that the biosensor could efficiently monitor the electrophysiological signals from taste bud organoids in response to various taste stimuli.
Article
Biochemistry & Molecular Biology
Yuanyuan Wang, Junjia Luan, Xuhua Tang, Wenhui Zhu, Yongxia Xu, Ying Bu, Jianrong Li, Fangchao Cui, Xuepeng Li
Summary: Umami peptides have attracted attention in the field of food umami science and are considered the key for umami agent development. In this study, virtual screening, molecular docking, and electronic tongue analysis were used to identify umami peptides from Atlantic cod myosin. Twenty-three putative umami peptides were screened and found to bind to the T1R3 cavity, exhibiting hydrogen bonding and electrostatic interactions. Twelve synthetic peptides tested on the electronic tongue showed umami taste and synergistic effects with monosodium glutamate. This study provides a method for rapid screening of umami peptides from marine protein resources and lays the foundation for their application in the food industry.
Article
Medicine, Research & Experimental
Wenwen Ren, Xudong Cha, Rui Xu, Tianyu Wang, Caiquan Liang, Janice Chou, Xiujuan Zhang, Fengzhen Li, Shenglei Wang, Boyu Cai, Peihua Jiang, Hong Wang, Huanhai Liu, Yiqun Yu
Summary: This study investigated the effects of cisplatin on taste cell homeostasis and gustatory function. It found that cisplatin inhibits cell proliferation and promotes apoptosis in taste buds, leading to impaired taste function and receptor cell generation. The study also identified critical genes and biological processes affected by chemotherapy and proposed a potential therapeutic target for taste dysfunction in cancer patients.
Article
Chemistry, Analytical
Jing-Xian Wang, Xue-Jin Yang, Yan-Zhai Wang, Kai Yang, Huayou Chen, Yang-Chun Yong
Summary: A signal amplification system for electrochemical sensing was established using bio-nanohybrid cells based on bacterial self-assembly and biomineralization. This system showed high transmembrane electron transfer efficiency and achieved ultrasensitive detection of riboflavin.
ANALYTICAL CHEMISTRY
(2022)
Article
Immunology
Katharina Radakovics, Claire Battin, Judith Leitner, Sabine Geiselhart, Wolfgang Paster, Johannes Stoeckl, Karin Hoffmann-Sommergruber, Peter Steinberger
Summary: Toll-like receptors (TLRs) are essential for recognizing microbial components and initiating immune responses. Although impurities containing TLR ligands can be problematic, this study developed a highly sensitive platform for detecting TLR ligands. By using specific reporter cell lines, the study demonstrated high specificity and sensitivity in detecting various TLR ligands. This system can be used to characterize different TLR ligands, including complex samples like bacterially expressed proteins or allergen extracts, and can help define the structural requirements for TLR agonists.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Chemistry, Analytical
Kunio Shimada
Summary: In order to develop versatile sensors capable of multiple responses to different sensations, researchers investigated mechanoreceptors fabricated as a single platform with an electric circuit. The proposed hybrid fluid rubber mechanoreceptors mimicking bio-inspired senses proved to be useful for resolving the complicated structure. Through electrochemical impedance spectroscopy, the intrinsic structure and physical mechanisms of firing rates of the mechanoreceptors were elucidated, with findings revealing the adaptions in different sensations.
Article
Chemistry, Multidisciplinary
Jun Young Hwang, Kyung Ho Kim, Sung Eun Seo, Youngju Nam, Sanghee Jwa, Inwoo Yang, Tai Hyun Park, Oh Seok Kwon, Seung Hwan Lee
Summary: A bioelectronic tongue system is developed to characterize the agonism and antagonism of bitter taste receptors through the combination of human bitter taste receptors with nanodiscs and graphene field-effect transistors. The system can sensitively detect bitter agonists and evaluate antagonism-based bitterness masking. This is of great importance in the food and pharmaceutical industries.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Engineering, Electrical & Electronic
Hengyang Wang, Xianghao Zhan, Li Liu, Asif Ullah, Huiyan Li, Han Gao, You Wang, Ruifen Hu, Guang Li
Summary: This study investigates the improvement of generalizability and transferability of taste sensation models developed with sEMG data by innovatively applying two methods: domain regularized component analysis (DRCA) and conformal prediction with shrunken centroids (CPSC). The effectiveness of these methods is explored in an unlabeled data augmentation process on six subjects. The results show that DRCA significantly improves classification accuracy, while CPSC does not guarantee accuracy improvement. The combination of DRCA and CPSC presents a statistically significant improvement in classification accuracy on six subjects.
IEEE TRANSACTIONS ON INSTRUMENTATION AND MEASUREMENT
(2022)
Article
Multidisciplinary Sciences
Rachel H. Parkinson, Sebastien C. Kessler, Jennifer Scott, Alexander Simpson, Jennifer Bu, Mushtaq Al-Esawy, Adam Mahdi, Ashwin Miriyala, Geraldine A. Wright
Summary: Research has found that the temporal firing patterns of bumblebees' gustatory receptor neurons can separate sugars into four distinct groups that correlate with sugar nutritional value and palatability. Additionally, another gustatory receptor neuron responds to high concentrations of fructose, sucrose, and maltose. The study also shows that nonmetabolizable or toxic sugars inhibit the bumblebees' responses to sucrose, indicating the refinement of their sweet taste for precise calculations of floral nectar's nature and nutritional value.
Article
Chemistry, Analytical
Merve Yilmaz, Monireh Bakhshpour, Ilgim Gokturk, Ayse Kevser Piskin, Adil Denizli
Summary: The heterogeneity and metastatic features of cancer cells lead to many casualties worldwide, making the development of a simple and effective diagnostic system to detect molecular markers of cancer crucial. Researchers have developed a specific and simple quartz crystal microbalance system to identify HER2/neu expressing breast cancer cells using a receptor-specific monoclonal antibody.
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)