Article
Instruments & Instrumentation
Takuma Sakaki, Kaori Masuda, Zixin Zhang, Tatsuhiko Yajima, Hijiri Hasegawa, Yeji Kim, Osamu Niwa
Summary: In this study, we utilized electrochemical surface plasmon resonance (EC-SPR) measurement to quantitatively evaluate the surface of a DNA sensor. By establishing the relationship between the charge induced by electrochemical desorption and the SPR angle shift, we were able to accurately assess the density of DNA probes and evaluate the hybridization efficiency of target DNA.
SENSORS AND MATERIALS
(2022)
Article
Chemistry, Analytical
Suzuyo Inoue, Kenta Fukada, Katsuyoshi Hayashi, Michiko Seyama
Summary: This study developed a novel measuring and data-processing method for electrochemical surface plasmon resonance (EC-SPR), which can be used to detect sensor surfaces that are difficult to measure a specific SPR angle, especially for samples with non-uniform thickness and coloration like polymers. The proposed method combines SPR measurements with an electrochemical reaction to measure small molecules without increasing the number of measurement steps.
Article
Polymer Science
Yi-Jung Lu, Han-Yun Hsieh, Wen-Chang Kuo, Pei-Kuen Wei, Horn-Jiunn Sheen, Hidetoshi Tahara, Te-Wei Chiu, Yu-Jui Fan
Summary: The study proposed a multiplex detection system by integrating LSPR sensing array and microfluidic channels, proving successful in detecting different targets in parallel channels.
Article
Chemistry, Analytical
Maria Lykova, Iuliana Panchenko, Martin Schneider-Ramelow, Tadatomo Suga, Fengwen Mu, Roy Buschbeck
Summary: This research paper presents a method to passivate Cu using a self-assembled monolayer (SAM) to protect against oxidation. The results show that SAM significantly reduces Cu oxidation and improves Cu-Cu bonding technology.
Article
Optics
Jingwen Feng, Jinjuan Gao, Wen Yang, Runcheng Liu, Muhammad Shafi, Zhipeng Zha, Cong Liu, Shicai Xu, Tingyin Ning, Shouzhen Jiang
Summary: This paper presents the fabrication of a D-shaped plastic optical fiber (D-POF) LSPR sensor based on a monolayer graphene/gold nanoparticles (Gr(m)/Au NPs) three-dimensional (3D) hybrid structure. The number of plasmonic structure layers was found to be important for sensor performance, with the optimal electromagnetic field enhancement effect observed in a three-layer plasmonic structure. The sensor exhibited outstanding sensitivity, linearity, and reproducibility, and successfully detected the concentration of glucose, suggesting promising applications in medicine and biotechnology.
Article
Biophysics
Richard B. M. Schasfoort, Jos van Weperen, Margot van Amsterdam, Judicael Parisot, Jan Hendriks, Michelle Koerselman, Marcel Karperien, Anouk Mentink, Martin Bennink, Hans Krabbe, Leon W. M. M. Terstappen, A. H. Leontine Mulder
Summary: The SPRi assay revealed that levels and binding strength of IgG antibodies against the RBD protein increased during the course of the disease in COVID-19 patients, while levels of IgM and IgA generally decreased. Recovered patients exhibited high binding strength of IgG antibodies to the RBD protein.
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Chemistry, Analytical
Domas Balciunas, Deivis Plausinaitis, Vilma Ratautaite, Almira Ramanaviciene, Arunas Ramanavicius
Summary: This research utilized molecular imprinting technology to create a glyphosate-sensitive layer for an electrochemical surface plasmon resonance (ESPR) sensor. The study revealed the restrictions and challenges encountered during the development of glyphosate ESPR sensor using molecularly imprinted polymer. It was found that glyphosate interfered with the deposition process of the polymer, but the problem was partially overcome by using a self-assembled monolayer before deposition. The dissociation constant and free energy of Gibbs values for the glyphosate-MIPpy interaction were calculated.
Article
Chemistry, Analytical
Shu-Cheng Lo, Shao-Sian Li, Wen-Fai Yang, Kuang-Chong Wu, Pei-Kuen Wei, Horn-Jiunn Sheen, Yu-Jui Fan
Summary: This paper presents a new micro/nanostructure co-hot embossing technique for the integration of gold-capped nanostructures as localized surface plasmon resonance (SPR) sensors into a microfluidic channel. The advantage of this technique is that it eliminates the need for alignment between the SPR sensors and the microfluidic channel during bonding. The integrated SPR sensor and microfluidic channel were characterized, and the sensitivity of the SPR sensor to refractive index was determined using different concentrations of glycerol solutions. The SPR sensor was also used for quantifying latent membrane protein (LMP-1) by modifying anti-LMP-1 on its surface, and a calibration curve was created using different concentrations of LMP-1 samples.
Article
Chemistry, Analytical
Chen-Ji Huang, Gudivada Vijaya Narasimha, Yu-Cheng Chen, Jen-Kun Chen, Guo-Chung Dong
Summary: The study focuses on evaluating the biological-physical interaction of micro-plastics with health cells and developing the movement mode and low concentration detection of micro-plastics using SPR technology. The research found that the movement mode of micro-plastics on estrogen receptors exhibited a rolling mode with certain characteristics and binding force.
Review
Chemistry, Analytical
Maria Simone Soares, Miguel Vidal, Nuno F. Santos, Florinda M. Costa, Carlos Marques, Sonia O. Pereira, Catia Leitao
Summary: The evolution of optical fiber technology has revolutionized various fields, especially in the field of biosensing. Different optical fiber configurations and biofunctionalization strategies affect the manufacturing complexity, cost, and performance of optical fiber immunosensors. Current research trends mainly focus on the application of technologies such as EW, SPR, LSPR, as well as the research and optimization of optical fiber configurations.
Review
Chemistry, Inorganic & Nuclear
Abin Philip, A. Ruban Kumar
Summary: The necessity for the development of highly sensitive and selective detection techniques for the investigation of biomolecules is inevitable. Surface plasmon resonance (SPR) based biosensor has evolved as a sustainable and commercially applicable tool for this investigation. Exploring real-time, cost-effective, and time-efficient detection techniques is crucial for improving quality of life.
COORDINATION CHEMISTRY REVIEWS
(2022)
Article
Biochemical Research Methods
Sepideh Fathi, Nazila Jalilzadeh, Mohammad Amini, Dariush Shanebandi, Behzad Baradaran, Fatemeh Oroojalian, Ahad Mokhtarzadeh, Prashant Kesharwani, Amirhossein Sahebkar
Summary: Due to its high mortality rates, typhoid fever continues to be a major health problem worldwide, especially in developing countries. However, the lack of accurate diagnostic tests and the similarity of typhoid symptoms to other diseases pose a challenge in accurately diagnosing typhoid fever. To address this issue, researchers have developed a Surface Plasmon Resonance (SPR) based biosensor that can specifically detect Salmonella typhi through DNA hybridization. The results showed that the biosensor could detect synthetic target sequences at a concentration of 10 nM and PCR products at a concentration of 1 nM. Additionally, the biosensor was able to accurately distinguish between complementary sequences and sequences with one base mismatch. The chip's surface can be regenerated and reused for consecutive diagnoses using NaOH solution. These findings highlight the potential of the designed biosensor for the detection of Salmonella typhi.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Materials Science, Multidisciplinary
Su-Kyoung Lee, Bora Yim, Jinseul Park, Nam-Gun Kim, Byung-Soo Kim, Yongdoo Park, Young Kyung Yoon, Jongseong Kim
Summary: The efficacy of COVID-19 vaccination relies on the levels of neutralizing antibodies in the serum. A bioresponsive nanogel-based surface plasmon resonance (nSPR) platform has been developed for rapid and quantitative detection of SARS-CoV-2 neutralizing antibodies without complicated pretreatment. This assay provides a selective and efficient method for detecting and quantifying neutralizing antibodies in serum samples.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Materials Science, Multidisciplinary
Su-Kyoung Lee, Bora Yim, Jinseul Park, Nam-Gun Kim, Byung-Soo Kim, Yongdoo Park, Young Kyung Yoon, Jongseong Kim
Summary: The efficacy of COVID-19 vaccination relies on the levels of SARS-CoV-2-neutralizing antibodies (NAbs) in the serum. A bioresponsive nanogel-based surface plasmon resonance (nSPR) platform has been developed to detect SARS-CoV-2 NAbs in clinical samples without complicated pretreatment. The nanogel-based NAb test shows excellent selectivity, rapid detection, and quantification of NAbs in each individual, providing a safer and easier NAb detection platform for COVID-19 vaccination strategies.
ACS APPLIED POLYMER MATERIALS
(2023)
Article
Chemistry, Multidisciplinary
Baeck B. Choi, Bethy Kim, Yiqi Chen, Sung Jong Yoo, Younghyun Cho, Peng Jiang
Summary: In this study, the colloidal Langmuir-Blodgett coating process was used to fabricate Au-covered silica sphere monolayers and investigate the impact of silica diameter on surface plasmon resonance sensing sensitivity. The research found that larger silica sphere diameters and dipole mode exhibited higher sensitivity in surface plasmon resonance sensing applications.
JOURNAL OF INDUSTRIAL AND ENGINEERING CHEMISTRY
(2021)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)