期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 9, 页码 2892-2897出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2009.02.025
关键词
Microfluidic device; Microelectrode; Negative dielectrophoresis; Leucocyte; Antibody; Selective capture
类别
资金
- NEDO of Japan
- Ministry of Education, Science, and Culture, Japan [20310070]
- Japan Society
- Grants-in-Aid for Scientific Research [20310070] Funding Source: KAKEN
We describe a microfluidic method to selectively capture a type of leucocytes (neutrophils or eosinophils) from a suspension of mixed leucocytes in a specific region of a microchannel whose upper wall is equipped with an array of microband electrodes. One of the microelectrodes is used for locally electrogenerating hypobromous acid (HBrO) that renders the opposite face of the channel protein-adsorptive. Since the patterned layer of poly(ethylene glycol)dimethacrylate (PEGDM), impervious to HBrO, is previously introduced at the channel bottom, the region of protein adsorption can be precisely defined even in the presence of fluid-flow. After capture antibody was immobilized on the treated region, an AC voltage (1 MHz, 20 V-pp) was applied to the microelectrode array for negative dielectrophoresis (DEP) to quickly concentrate cells from the flowing cell suspension. By immobilizing neutrophil- or eosinophil-specific antibodies onto the created region, we demonstrated that the corresponding leucocyte type can be captured from the concentrated leucocytes. (C) 2009 Elsevier B.V. All rights reserved.
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