Article
Biophysics
Yonghwan Kim, Dongtak Lee, Youngjun Seo, Hyo Gi Jung, Jae Won Jang, Dongsung Park, Insu Kim, Jaeheung Kim, Gyudo Lee, Kyo Seon Hwang, Seung-Hyun Kim, Sang Won Lee, Jeong Hoon, Dae Sung Yoon
Summary: In this study, an electrochemical biosensor based on cell membrane was developed, inspired by the pathological process of cholera toxin. The biosensor exhibited excellent detection performance, selectivity, and long-term stability. Overall, it has great potential for clinical application and cost-effective detection of cholera toxin in developing countries.
BIOSENSORS & BIOELECTRONICS
(2023)
Article
Nanoscience & Nanotechnology
Emily A. Schafer, Eliana Davis, Zachary Manzer, Susan Daniel, Jonathan Rivnay
Summary: A new class of biosensors utilizes native transmembrane proteins in biomimetic membranes to improve the detection of electrochemical signals. Blending native phospholipids with synthetic block copolymers to create a hybrid lipid bilayer addresses the stability and limited membrane properties of traditional phospholipid bilayers. The inclusion of block copolymers enhances bilayer resilience and offers important benefits for bio-hybrid bioelectronics.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Chemistry, Analytical
Karutha Pandian Divya, Shanmuganathan Keerthana, Chinnuswamy Viswanathan, Nagamony Ponpandian
Summary: This paper reports a biomimetic bilayer lipid membrane supported MXene based biosensor for electrochemical hybridization detection of the most prevalent and potential breast cancer biomarker BRCA1. A 2D MXene nanosheet-anchored gold nanoparticle-decorated biomimetic bilayer lipid membrane (AuNP@BLM) biosensor is used for the attachment of thiolated single-stranded DNA (HS-ssDNA) targeting hybridization detection. The synergistic combination of MXene and AuNP@BLM has proven to efficiently improve the detection signal to several folds.
Article
Cell Biology
Stefanie S. Schmieder, Raju Tatituri, Michael Anderson, Kate Kelly, Wayne I. Lencer
Summary: This study investigates how the acyl chain structures of complex sphingolipids influence their secretion and intracellular distribution, and finds that lysosomal sorting is achieved by excluding the endocytic pathway sorting tubules. Additionally, cholesterol and unsaturation of the acyl chains also impact the sorting of sphingolipids.
Article
Multidisciplinary Sciences
Elena -Sofia Heinl, Sebastian Lorenz, Barbara Schmidt, Nouf Nasser M. Laqtom, Joseph R. Mazzulli, Laetitia Francelle, Timothy W. Yu, Benjamin Greenberg, Stephan Storch, Ines Tegtmeier, Helga Othmen, Katja Maurer, Malin Steinfurth, Ralph Witzgall, Vladimir Milenkovic, Christian H. Wetzel, Markus Reichold
Summary: The study found that CLN7 may be involved in the infection process of the SARS-CoV-2 virus. CLN7 deficiency leads to a significant reduction in viral load, while overexpression of CLN7 increases viral load. This research provides a potential target for drug development against COVID-19 and helps us understand the physiological function of CLN7.
Review
Food Science & Technology
Numa Piot, F. Gisou van der Goot, Oksana A. Sergeeva
Summary: AB toxins are bacterial two-component systems that utilize protein-based mechanisms to deliver toxins, providing insights into the development of bio-based drug delivery systems. With their intrinsic properties and modular characteristics, these toxins have great potential in therapy, allowing for the development of new therapeutic strategies and serving as promising tools in drug and drug delivery system development.
Article
Chemistry, Physical
Jie Lian, Jinyu Zhao, Xiaomin Wang, Qiang Bai
Summary: Rational design of dual-sites catalysts is crucial for promoting the sluggish kinetics of oxygen reduction reactions. This study successfully synthesized a novel dual-sites electrocatalyst with dispersed Co-Nx and Se-C dual sites embedded in ZIF-derived porous carbon frameworks, which showed positive oxygen reduction reaction activity. Moreover, the catalyst displayed high activity and low charge-transfer resistance in fuel cell tests.
Article
Chemistry, Multidisciplinary
Ye Zeng, Mengjie Shen, Ankush Singhal, Geert Jan Agur Sevink, Niek Crone, Aimee L. L. Boyle, Alexander Kros
Summary: An ideal nanomedicine system improves drug therapy by enhancing cellular uptake. However, current nanomedicines mainly enter cells through endosomal/lysosomal pathways, leading to inefficient delivery of therapeutic cargo. To overcome this issue, the authors developed a synthetic lipidated peptide pair, E4/K4, inspired by natural fusion machinery. By optimizing the structure of the peptide, the researchers achieved enhanced membrane fusion and efficient delivery of drugs into cells. This study provides valuable insights for the development of efficient drug delivery systems based on liposome-cell fusion strategies.
Article
Chemistry, Physical
Shuang Yang, Ji-Yu Zhu, Xiao-Nan Chen, Meng-Jie Huang, Sheng-Hao Cai, Ji-Yuan Han, Ji-Sen Li
Summary: In this study, a new catalyst Ni2P-Ru2P/NF was reported, which showed excellent electrocatalytic performance for the oxygen and hydrogen evolution reactions in alkaline media. Additionally, it demonstrated fast catalytic performance for full water splitting with a low cell voltage.
APPLIED CATALYSIS B-ENVIRONMENTAL
(2022)
Article
Engineering, Chemical
Long Tian, Nigel Graham, Ting Liu, Kening Sun, Wenzheng Yu
Summary: This paper describes the development and testing of a dual-site supported graphene oxide (GO) membrane, which introduces biuret and Mg2+ inside the interlayer structure to improve water flux and separation performance. The membrane exhibits a high selectivity towards dye molecules and improved stability compared to other types of cross-linked GO membranes.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Ryugo Tero, Yoshi Hagiwara, Shun Saito
Summary: The gel-phase domains preferentially distributed on graphene oxide (GO) flakes in a binary supported lipid bilayer, while the liquid crystalline phase increased on the bare SiO2 surface. The gel-phase domain was condensed more effectively on GO at the lower cooling rate.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Xiangzhao Ai, Dan Wang, Ilkoo Noh, Yaou Duan, Zhidong Zhou, Nilesh Mukundan, Ronnie H. Fang, Weiwei Gao, Liangfang Zhang
Summary: This study reports a novel detoxification strategy against botulinum toxin (BoNT) using glycan-modified cellular nanosponges. The study demonstrates that higher levels of gangliosides on the cellular nanosponges result in greater binding capacities with BoNT, showing superior efficacy in neutralizing BoNT cytotoxicity in vitro and in a mouse model of BoNT intoxication. This research highlights the potential of cellular nanosponges, especially when modified with glycans, as a promising countermeasure platform against BoNT and related clostridial toxins.
Review
Chemistry, Physical
Hassiba Smida, Christine Thobie-Gautier, Mohammed Boujtita, Estelle Lebegue
Summary: Single liposome electrochemistry is a sensitive and versatile tool for understanding biological processes and mechanisms at the lipid membrane/electrode interface. It offers the opportunity to detect various biotargets with high sensitivity in a short time, but it also has limitations.
CURRENT OPINION IN ELECTROCHEMISTRY
(2022)
Review
Biochemistry & Molecular Biology
Toshiyuki Tosaka, Koki Kamiya
Summary: Membrane proteins are crucial for various cellular functions, and their structural and functional studies are important in fields like biology, medicine, pharmacology, biotechnology, and bioengineering. However, observing their precise reactions and structures is challenging. This paper introduces methods for creating liposomes or lipid vesicles and techniques for reconstituting membrane proteins into artificial membranes. It also discusses different types of artificial membranes used to observe the functions of reconstituted membrane proteins, and the reconstitution and function of multiple membrane proteins using a cell-free synthesis system.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Yuri N. Antonenko, Marta I. Jedrzejczyk, Tatyana I. Rokitskaya, Ljudmila S. Khailova, Elena A. Kotova, Adam Huczynski
Summary: A series of SAL derivatives targeting mitochondria were synthesized, and compound 5 exhibited promising anticancer and antimicrobial activity with high permeability on lipid membranes.
BIOELECTROCHEMISTRY
(2022)
Article
Biophysics
Reza Khodadadi, Manouchehr Eghbal, Hamideh Ofoghi, Alireza Balaei, Ali Tamayol, Karen Abrinia, Amir Sanati-Nezhad, Mohamadmahdi Samandari
Summary: This paper introduces an integrated portable centrifugal microfluidic system that automates cell and fluid manipulation for complete blood counting (CBC) analysis at the point-of-care (POC). The system utilizes a specially designed microfluidic disc for cell separation, solution metering and mixing, and cell counting, and is equipped with a custom script for automated quantification of cells. The proposed method shows a strong correlation with the gold standard hematology analyzer for various blood parameters. The portable system offers simplicity, affordability, and low power consumption, making it a potential solution for improving healthcare delivery in resource-limited settings and remote areas.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Nabil H. Bhuiyan, Joon S. Shim
Summary: Developing herd immunity is crucial for changing the course of the COVID-19 pandemic. An AI-driven point-of-care testing platform has been proposed for analyzing the body's response to SARS-CoV-2, and it has been successfully used for quantifying viral proteins and antibodies in blood samples. A data-receptive web-based dashboard system has also been developed for real-time analysis of herd immunity progress.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Alexiane Godain, Timothy M. Vogel, Pascal Fongarland, Naoufel Haddour
Summary: This study provides comprehensive insights into the intricate relationship between shear stress and electroactive biofilms in microbial fuel cells, highlighting the pivotal role of shear stress in influencing the growth kinetics, electrical performance, and physical structure of the biofilms. The study also emphasizes the complexities of extracellular electron transfer mechanisms and the need for complementary metaproteomic analyses.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Linjie Wang, Yixin Chen, Yang Ji, Shujun Zheng, Fei Wang, Caolong Li
Summary: A paper-based biosensor incorporating surface-enhanced Raman spectroscopy (SERS) and colorimetric detection has been developed for efficient quantification of intracellular glutathione (GSH). The biosensor exhibits excellent selectivity, stability, and precision, with low detection limits in both SERS and colorimetric modes. It has been successfully utilized for intracellular GSH detection and validated against a commercial GSH assay kit.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Quan Zhou, Jing Wang, Zhen Zhang, Alain Wuethrich, Richard J. Lobb, Matt Trau
Summary: This study presents a biosensor for sensitive detection of EMT-associated biomarkers on extracellular vesicles (EVs) surfaces during targeted therapy. Through longitudinal monitoring of patients, the biosensor shows its ability to identify the EMT-like phenotype switching on circulating EVs during the development of resistance.
BIOSENSORS & BIOELECTRONICS
(2024)
Review
Biophysics
Pratika Rai, Sabrina N. Hoba, Celine Buchmann, Robert J. Subirana-Slotos, Christian Kersten, Tanja Schirmeister, Kristina Endres, Bernd Bufe, Alexey Tarasov
Summary: Proteases have been proposed as potential biomarkers for various pathological conditions. The development of protease biosensors offers a more efficient way to investigate dysregulated proteases. This review article presents different optical and electrochemical detection methods for designing biosensors for all major protease families.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Chengxin Liu, Xu Shen, Li Yan, Runlian Qu, Yue Wang, Yaqin He, Zixuan Zhan, Piaopiao Chen, Feng Lin
Summary: In this study, a homogeneous rapid electrochemical aptasensor was developed to quantitatively detect CTCs in lung cancer patients. The aptasensor utilized a DNA nanosphere structure and a complementary aptamer to specifically detect mucin 1 as a marker for CTCs. The assay exhibited high specificity and sensitivity, and the results were consistent with other detection methods.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Danni Xue, Xing Dai, Jialong Zhao, Jiayao Zhang, Huan Liu, Kui Liu, Tao Xu, Chenjie Gu, Xingfei Zhou, Tao Jiang
Summary: In this study, a dual-enhancement SERS substrate based on Ti3C2TX and Ag nanocubes was fabricated for precise quantification of ritonavir and ibrutinib in serum. The formation of numerous electromagnetic hotspots between Ag nanocubes facilitated effective photo-induced charge transfer. The composite substrate showed excellent sensitivity, achieving low detection limits and high recoveries, making it promising for monitoring and identification of clinical blood drug concentration.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Tenghua Zhang, Zihui Xie, Xiaohe Zheng, Yuxin Liang, Yao Lu, Hankang Zhong, Feiyang Qian, Yuqing Zhu, Ruiting Sun, Yan Sheng, Jiaming Hu
Summary: This study reports a technology based on cationic lipid-polymer hybrid nanoparticles for efficient extracellular vesicle (EV) enrichment and in-situ detection of internal microRNAs. The technology demonstrates high EV enrichment efficiency and sensitive internal RNA detection, making it potentially useful for early pancreatic cancer diagnosis.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Wenjie Dai, Gaoxu Chen, Xiaoyan Wang, Shujun Zhen, Chengzhi Huang, Lei Zhan, Yuanfang Li
Summary: In this study, a novel metal organic gel (MOG) with dual electrochemiluminescence (ECL) properties was prepared by simple mixing. The MOG exhibited strong and stable anodic and cathodic ECL signals. By utilizing this MOG, an ECL resonance energy transfer (ECL-RET) biosensor was constructed for ultrasensitive detection of a specific gene. The study developed a straightforward technique for obtaining a single luminescent material with dual signals and expanded the analytical application of MOGs in the realm of ECL.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Lais Canniatti Brazaca, Amanda Hikari Imamura, Rodrigo Vieira Blasques, Jessica Rocha Camargo, Bruno Campos Janegitz, Emanuel Carrilho
Summary: The use of microfluidic paper-based analytical devices (muPADs) for medical diagnosis is a growing trend due to their low cost, easy use, simple manufacturing, and potential for application in low-resource settings. This review focuses on the advances in muPADs for medical diagnostics, discussing their use in detecting various biomarkers in common human biofluids. The challenges of biomarker detection in each sample are examined, along with innovative techniques to overcome these limitations. The commercialization difficulties of muPADs are also considered, along with future trends such as wearable devices and integrated platforms.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Minghong Jian, Xudong Sun, Hua Zhang, Xiaotong Li, Shasha Li, Zhenxin Wang
Summary: Matrix metalloproteinases (MMPs) are attractive biomarkers for cancer diagnosis and treatment, but their low abundance in biological samples, especially in the early stages of tumors, makes it challenging to precisely analyze MMP activities. In this study, a peptide microarray-based metal-enhanced fluorescence assay (PMMEFA) is proposed as a sensitive and specific method to simultaneously detect MMP-1, -2, -3, -7, -9, and -13 activities. The PMMEFA showed excellent sensitivity and was capable of detecting MMP activities in various matrices.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Kyung Ho Kim, Eunsu Ryu, Zinah Hilal Khaleel, Sung Eun Seo, Lina Kim, Yong Ho Kim, Hyun Gyu Park, Oh Seok Kwon
Summary: We have developed a novel strategy for discriminative detection of SARS-CoV-2 variants using the plasmonic photothermal effect of gold nanofilms and digital polymerase chain reaction (dPCR) technology. With this method, we were able to detect the delta and omicron variants with high sensitivity within 25 minutes from COVID-19 patients' clinical samples, making it a rapid and accurate point-of-care testing tool.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Zepeng Kang, Yuanming Wang, Haiyan Song, Xueli Wang, Job Zhang, Zhiguang Zhu
Summary: By designing a wearable and flexible lactic-acid/O2 EBFC with an air-breathing biocathode, the limitations of biocathode are effectively solved. The optimal performance conditions are determined through experiments, and the EBFC is successfully applied to power a low-power device.
BIOSENSORS & BIOELECTRONICS
(2024)
Article
Biophysics
Huayun Chen, Zhiheng You, Yuhui Hong, Xiao Wang, Mingming Zhao, Yushi Luan, Yibin Ying, Yixian Wang
Summary: This study developed a colorimetric sensor array using gas-responsive two-dimensional metal-organic framework (MOF) composites for highly sensitive detection of volatile organic compounds (VOCs). The dye/Zn-2(bim)(4) composites-based sensor arrays showed enhanced sensitivity and anti-interference properties. The findings demonstrate the potential use of dye/Zn-2(bim)(4) sensor arrays for early perception of plant diseases.
BIOSENSORS & BIOELECTRONICS
(2024)