期刊
BIOSENSORS & BIOELECTRONICS
卷 24, 期 4, 页码 825-830出版社
ELSEVIER ADVANCED TECHNOLOGY
DOI: 10.1016/j.bios.2008.07.008
关键词
Gold-nanoparticles; Aptamers; Thrombin; Stripping voltammetry; Electrochemical aptasensor
类别
资金
- Federal Agency of Science and innovations Ministry of Education and Science of Russian Federation [02.512.11.2105]
- Interdepartmental Program Proteomics in Medicine and Biotechnology
A novel electrochemical method for the detection of bioaffinity interactions based on a gold-nanoparticles sensing platform and on the usage of stripping voltammetry technique was developed. The oxidation of gold surface (resulted in gold oxide formation) upon polarization served as a basis for analytical response. As a model, thrombin-thrombin binding aptamer couple was chosen. The aptamer was immobilized on a screen-printed electrode modified with gold-nano particles by avidin-biotin technology. Cathodic peak area was found proportional to thrombin quantity specifically adsorbed onto electrode surface. Sigmoid calibration curve as is typical for immunoassay was obtained, with thrombin detection limit of 10(-9) M. Linear range corresponds from 10(-8) to 10(-5) M thrombin concentration or 2 x 10(-14) to 2 x 10(-11) mol/electrode (R = 0.996). Binding of thrombin to an aptamer has also been detected using the ferricyanide/ferrocyanide redox couple as electrochemical indicator. (C) 2008 Published by Elsevier B.V.
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