期刊
BIOSCIENCE REPORTS
卷 34, 期 -, 页码 257-U243出版社
PORTLAND PRESS LTD
DOI: 10.1042/BSR20140051
关键词
DNA-dependent protein kinase; midbody; mitosis; polo-like protein kinase 1; protein phosphatase 6
资金
- Cancer Research Society [1022480]
- Canadian Institutes of Health Research [MOP13639]
- Public Health Service [AI048758]
- Cancer Research UK
- Engineered Air Chair in Cancer Research
The protein kinase activity of the DNA-PKcs (DNA-dependent protein kinase catalytic subunit) and its autophosphorylation are critical for DBS (DNA double-strand break) repair via NHEJ (non-homologous end-joining). Recent studies have shown that depletion or inactivation of DNA-PKcs kinase activity also results in mitotic defects. DNA-PKcs is auto-phosphorylated on Ser(2056), Thr(2647) and Thr(2609) in mitosis and phosphorylated DNA-PKcs localize to centrosomes, mitotic spindles and the midbody. DNA-PKcs also interacts with PP6 (protein phosphatase 6), and PP6 has been shown to dephosphorylate Aurora A kinase in mitosis. Here we report that DNA-PKcs is phosphorylated on Ser(3205) and Thr(3950) in mitosis. Phosphorylation of Thr(3950) is DNA-PK-dependent, whereas phosphorylation of Ser(3205) requires PLK1 (polo-like kinase 1). Moreover, PLK1 phosphorylates DNA-PKcs on Ser(3205) in vitro and interacts with DNA-PKcs in mitosis. In addition, PP6 dephosphorylates DNA-PKcs at Ser(3205) in mitosis and after IR (ionizing radiation). DNA-PKcs also phosphorylates Chk2 on Thr(68) in mitosis and both phosphorylation of Chk2 and autophosphorylation of DNA-PKcs in mitosis occur in the apparent absence of Ku and DNA damage. Our findings provide mechanistic insight into the roles of DNA-PKcs and PP6 in mitosis and suggest that DNA-PKcs' role in mitosis may be mechanistically distinct from its well-established role in NHEJ.
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