期刊
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
卷 78, 期 8, 页码 1371-1375出版社
TAYLOR & FRANCIS LTD
DOI: 10.1080/09168451.2014.921557
关键词
Endocrine disruptors; bisphenol A; estrogenic activity; CDK1/2; p38 MAPK
类别
资金
- Ministry of Food and Drug Safety [12161MFDS741]
Bisphenol A (BPA) is considered to be an endocrine disruptor, but the mechanisms by which it disrupts endocrine functions are poorly understood. Here, we have shown that BPA binds both estrogen receptor (ER)-alpha and ER-beta (ER-beta) using a fluorescence polarization competitive binding assay. In addition, we found that BPA induced cell proliferation by modulating cell cycle-related genes in the MCF-7 human mammary cancer cell line. Moreover, using a BG1 luciferase ER transactivation assay, we found that BPA has estrogenic activity. Modulating the MAPK pathway by using an ERK inhibitor (PD98059) or a JNK inhibitor (SP600125) had no effect on the ability of BPA to induce estrogenic activity. However, the antiestrogen, ICI 182,780, and the p38 inhibitor, PD 169316 successfully blocked BPA-induced estrogenic activity. Our findings suggest that BPA mimics ER-dependent estrogenic activity by targeting proteins that regulate the cell cycle and p38 MAPK.
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